Transcript: Mouse XM_030254518.1

PREDICTED: Mus musculus mitogen-activated protein kinase 10 (Mapk10), transcript variant X7, mRNA.

Source:
NCBI, updated 2019-08-08
Taxon:
Mus musculus (mouse)
Gene:
Mapk10 (26414)
Length:
6415
CDS:
634..1806

Additional Resources:

NCBI RefSeq record:
XM_030254518.1
NBCI Gene record:
Mapk10 (26414)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Mouse XM_030254518.1, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Mouse Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000360388 GCGGATTCTGAGCACAATAAA pLKO_005 1369 CDS 100% 15.000 21.000 N Mapk10 n/a
2 TRCN0000360389 GTATGTGGTGACGCGATATTA pLKO_005 1077 CDS 100% 15.000 21.000 N Mapk10 n/a
3 TRCN0000194979 CCATTTCATGTGATCTATTAC pLKO.1 2426 3UTR 100% 13.200 18.480 N MAPK10 n/a
4 TRCN0000012633 GCGATAGAACACAGCACACAT pLKO.1 1850 3UTR 100% 4.950 6.930 N Mapk10 n/a
5 TRCN0000201207 GTTGGAACCAAGTCAACGTAT pLKO.1 6352 3UTR 100% 4.950 6.930 N Mapk10 n/a
6 TRCN0000360315 CAATAGAGAGATCCAACATAA pLKO_005 2007 3UTR 100% 13.200 10.560 N Mapk10 n/a
7 TRCN0000417287 ATGAAGTGTGTGAACCATAAA pLKO_005 754 CDS 100% 13.200 9.240 N MAPK10 n/a
8 TRCN0000191064 CCAAGTCAACGTATTGTAATT pLKO.1 6359 3UTR 100% 13.200 9.240 N Mapk10 n/a
9 TRCN0000012634 CGAAGAATGGAAAGAACTTAT pLKO.1 1572 CDS 100% 13.200 9.240 N Mapk10 n/a
10 TRCN0000196370 GACAGAAATGTGGCCATTAAG pLKO.1 670 CDS 100% 13.200 9.240 N MAPK10 n/a
11 TRCN0000012637 CAAGTCTGATTGCACACTGAA pLKO.1 1002 CDS 100% 4.950 3.465 N Mapk10 n/a
12 TRCN0000012635 CCAAGATGTCTACTTAGTGAT pLKO.1 828 CDS 100% 4.950 3.465 N Mapk10 n/a
13 TRCN0000432277 GCCTAGTCAGATGGATGTAGA pLKO_005 2306 3UTR 100% 4.950 3.465 N MAPK10 n/a
14 TRCN0000191558 GAATCATTAAAGCTGAAGGAA pLKO.1 6392 3UTR 100% 3.000 2.100 N Mapk10 n/a
15 TRCN0000001020 GCCATTAAGAAGCTCAGCAGA pLKO.1 682 CDS 100% 2.640 1.848 N MAPK10 n/a
16 TRCN0000001938 GCCATTAAGAAGCTCAGCAGA pLKO.1 682 CDS 100% 2.640 1.848 N MAPK10 n/a
17 TRCN0000166364 CACACACACACACACACACAA pLKO.1 3841 3UTR 100% 4.950 2.475 Y KAAG1 n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript XM_030254518.1, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 TRCN0000487812 TGCAGATTATTTTACTCCTCGACG pLX_317 20.5% 74.2% 71.3% V5 (not translated due to prior stop codon) (many diffs) n/a
2 TRCN0000488648 ACCAGGGTGAGTGAATCTAAGAAG pLX_317 20.3% 74.1% 71.3% V5 (not translated due to prior stop codon) (many diffs) n/a
3 ccsbBroadEn_11058 pDONR223 100% 74.1% 81% None (many diffs) n/a
4 ccsbBroad304_11058 pLX_304 0% 74.1% 81% V5 (many diffs) n/a
5 TRCN0000475774 GTGAGTTGCCAATCATGCAATTTA pLX_317 29% 74.1% 81% V5 (many diffs) n/a
6 ccsbBroadEn_11057 pDONR223 100% 72% 80% None (many diffs) n/a
7 ccsbBroad304_11057 pLX_304 0% 72% 80% V5 (many diffs) n/a
8 TRCN0000468220 CCCGCTATCGAACCGGGCATATAA pLX_317 45% 72% 80% V5 (many diffs) n/a
9 ccsbBroadEn_14805 pDONR223 0% 72% 80% None (many diffs) n/a
10 ccsbBroad304_14805 pLX_304 0% 72% 80% V5 (many diffs) n/a
11 TRCN0000466133 TCATAAGCAAACAGGTTCCCACAG pLX_317 29.1% 72% 80% V5 (many diffs) n/a
12 TRCN0000492117 CTAAGCTAACTACGGCGGTACGCC pLX_317 31.1% 65.2% 71.3% V5 (many diffs) n/a
13 TRCN0000491457 CCGTCCACGCTTTTACTCGGCAGT pLX_317 24.4% 65.2% 71.3% V5 (not translated due to prior stop codon) (many diffs) n/a
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