Construct: ORF TRCN0000480225
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF002810.3_s317c1
- Derived from:
- ccsbBroadEn_14564
- DNA Barcode:
- GACAGTGGGGGTGATCTCGGAGCG
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- CAMK2B (816)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000480225
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 816 | CAMK2B | calcium/calmodulin dependen... | NM_172081.3 | 100% | 100% | |
| 2 | human | 816 | CAMK2B | calcium/calmodulin dependen... | XM_017012664.1 | 99.8% | 99.8% | 943_944insCAG |
| 3 | human | 816 | CAMK2B | calcium/calmodulin dependen... | NM_172079.2 | 97.1% | 97.1% | 1059_1103del |
| 4 | human | 816 | CAMK2B | calcium/calmodulin dependen... | NM_172080.3 | 96.9% | 96.9% | 943_944insCAG;1056_1100del |
| 5 | human | 816 | CAMK2B | calcium/calmodulin dependen... | XM_005249864.3 | 96.7% | 95.8% | (many diffs) |
| 6 | human | 816 | CAMK2B | calcium/calmodulin dependen... | XM_017012665.1 | 96.5% | 95.6% | (many diffs) |
| 7 | human | 816 | CAMK2B | calcium/calmodulin dependen... | XM_005249862.3 | 95.3% | 95.2% | 947_1019delinsC |
| 8 | human | 816 | CAMK2B | calcium/calmodulin dependen... | NM_172083.3 | 95.2% | 95.2% | 986_987ins72 |
| 9 | human | 816 | CAMK2B | calcium/calmodulin dependen... | NM_001293170.2 | 92.7% | 92.6% | 947_1019delinsC;1131_1175del |
| 10 | human | 816 | CAMK2B | calcium/calmodulin dependen... | NM_172078.3 | 92.7% | 92.6% | 947_1019delinsC;1131_1175del |
| 11 | human | 816 | CAMK2B | calcium/calmodulin dependen... | XM_006715784.2 | 92.2% | 91.2% | (many diffs) |
| 12 | human | 816 | CAMK2B | calcium/calmodulin dependen... | NM_172084.3 | 89.2% | 89.2% | 944_945ins162 |
| 13 | human | 816 | CAMK2B | calcium/calmodulin dependen... | NM_172082.3 | 87.3% | 86.3% | (many diffs) |
| 14 | human | 816 | CAMK2B | calcium/calmodulin dependen... | XM_017012662.1 | 83.1% | 83.1% | 943_944insCAG;1056_1100del;1151_1408del |
| 15 | human | 816 | CAMK2B | calcium/calmodulin dependen... | XM_006715781.2 | 80% | 80% | 943_944insCAG;1106_1477del |
| 16 | human | 816 | CAMK2B | calcium/calmodulin dependen... | XM_024446945.1 | 80% | 79.9% | 947_1019delinsC;1131_1175del;1226_1483del |
| 17 | human | 818 | CAMK2G | calcium/calmodulin dependen... | NM_172173.3 | 79.4% | 87.7% | (many diffs) |
| 18 | human | 818 | CAMK2G | calcium/calmodulin dependen... | NM_001367530.1 | 78.7% | 87% | (many diffs) |
| 19 | human | 818 | CAMK2G | calcium/calmodulin dependen... | NM_001222.4 | 78.1% | 87.9% | (many diffs) |
| 20 | human | 818 | CAMK2G | calcium/calmodulin dependen... | XM_017016730.1 | 77.9% | 85.5% | (many diffs) |
| 21 | human | 818 | CAMK2G | calcium/calmodulin dependen... | NM_001367517.1 | 77.8% | 86.1% | (many diffs) |
| 22 | human | 818 | CAMK2G | calcium/calmodulin dependen... | NM_172170.5 | 77.8% | 86.1% | (many diffs) |
| 23 | human | 816 | CAMK2B | calcium/calmodulin dependen... | XM_017012661.1 | 76.6% | 76.5% | 947_1019delinsC;1179_1565del |
| 24 | human | 818 | CAMK2G | calcium/calmodulin dependen... | NM_001367532.1 | 76.5% | 84.5% | (many diffs) |
| 25 | human | 818 | CAMK2G | calcium/calmodulin dependen... | NM_001367531.1 | 76.4% | 83.9% | (many diffs) |
| 26 | human | 815 | CAMK2A | calcium/calmodulin dependen... | NM_001369025.2 | 76.3% | 81.1% | (many diffs) |
| 27 | human | 815 | CAMK2A | calcium/calmodulin dependen... | NM_001363990.1 | 76.2% | 81.1% | (many diffs) |
| 28 | human | 815 | CAMK2A | calcium/calmodulin dependen... | NM_171825.2 | 76.2% | 81.1% | (many diffs) |
| 29 | human | 818 | CAMK2G | calcium/calmodulin dependen... | NM_001367516.1 | 76% | 81% | (many diffs) |
| 30 | human | 818 | CAMK2G | calcium/calmodulin dependen... | NM_001367545.1 | 75.7% | 81.9% | (many diffs) |
| 31 | human | 818 | CAMK2G | calcium/calmodulin dependen... | NM_172169.3 | 75.6% | 83.5% | (many diffs) |
| 32 | human | 818 | CAMK2G | calcium/calmodulin dependen... | NM_001367536.1 | 75.4% | 82.4% | (many diffs) |
| 33 | human | 816 | CAMK2B | calcium/calmodulin dependen... | NM_001220.5 | 75.4% | 75.3% | 947_1019delinsC;1131_1175del;1226_1597del |
| 34 | human | 816 | CAMK2B | calcium/calmodulin dependen... | XM_011515555.1 | 75.4% | 75.3% | 947_1019delinsC;1131_1175del;1224_1595del |
| 35 | human | 816 | CAMK2B | calcium/calmodulin dependen... | XM_011515556.1 | 75.2% | 71.9% | (many diffs) |
| 36 | human | 816 | CAMK2B | calcium/calmodulin dependen... | XM_017012660.1 | 75% | 75% | 1107_1607del |
| 37 | human | 816 | CAMK2B | calcium/calmodulin dependen... | XM_011515557.1 | 75% | 71.8% | (many diffs) |
| 38 | human | 818 | CAMK2G | calcium/calmodulin dependen... | XM_005270205.1 | 75% | 84.4% | (many diffs) |
| 39 | human | 818 | CAMK2G | calcium/calmodulin dependen... | XM_006717998.1 | 74.9% | 82.2% | (many diffs) |
| 40 | human | 816 | CAMK2B | calcium/calmodulin dependen... | XM_011515554.1 | 74.9% | 74.9% | 943_944insCAG;1104_1604del |
| 41 | human | 816 | CAMK2B | calcium/calmodulin dependen... | XM_011515553.1 | 74.9% | 74.8% | 947_1019delinsC;1131_1175del;1224_1610del |
| 42 | human | 818 | CAMK2G | calcium/calmodulin dependen... | NM_001204492.1 | 74.8% | 82.7% | (many diffs) |
| 43 | human | 818 | CAMK2G | calcium/calmodulin dependen... | XM_024448218.1 | 74.3% | 82% | (many diffs) |
| 44 | human | 816 | CAMK2B | calcium/calmodulin dependen... | XM_017012670.2 | 74.3% | 74.3% | 0_1ins387 |
| 45 | human | 818 | CAMK2G | calcium/calmodulin dependen... | NM_001367518.1 | 74.3% | 81.1% | (many diffs) |
| 46 | human | 818 | CAMK2G | calcium/calmodulin dependen... | NM_001367533.1 | 74% | 81.8% | (many diffs) |
| 47 | human | 818 | CAMK2G | calcium/calmodulin dependen... | NM_001367543.1 | 73.5% | 80.7% | (many diffs) |
| 48 | human | 816 | CAMK2B | calcium/calmodulin dependen... | XM_011515550.1 | 73.4% | 73.4% | 1059_1103del;1152_1652del |
| 49 | human | 816 | CAMK2B | calcium/calmodulin dependen... | XM_011515552.1 | 73.2% | 73.2% | 943_944insCAG;1056_1100del;1149_1649del |
| 50 | human | 816 | CAMK2B | calcium/calmodulin dependen... | XM_011515558.2 | 73% | 61.3% | (many diffs) |
| 51 | human | 818 | CAMK2G | calcium/calmodulin dependen... | NM_001367527.1 | 72.8% | 78.9% | (many diffs) |
| 52 | human | 818 | CAMK2G | calcium/calmodulin dependen... | NM_172171.2 | 72.7% | 80.4% | (many diffs) |
| 53 | human | 816 | CAMK2B | calcium/calmodulin dependen... | XM_011515551.1 | 72.5% | 69.3% | (many diffs) |
| 54 | human | 816 | CAMK2B | calcium/calmodulin dependen... | XM_011515549.1 | 72.4% | 72.3% | 947_1019delinsC;1179_1679del |
| 55 | human | 816 | CAMK2B | calcium/calmodulin dependen... | XM_017012668.2 | 72.2% | 72.2% | 0_1ins387;672_716del |
| 56 | human | 816 | CAMK2B | calcium/calmodulin dependen... | XM_024446946.1 | 72% | 72% | 0_1ins387;556_557insCAG;669_713del |
| 57 | human | 818 | CAMK2G | calcium/calmodulin dependen... | NM_001367547.1 | 71.8% | 78.9% | (many diffs) |
| 58 | human | 818 | CAMK2G | calcium/calmodulin dependen... | NM_001367546.1 | 71.5% | 78.1% | (many diffs) |
| 59 | human | 818 | CAMK2G | calcium/calmodulin dependen... | NM_001367525.1 | 71.5% | 78.9% | (many diffs) |
| 60 | human | 818 | CAMK2G | calcium/calmodulin dependen... | XM_006717996.1 | 71.5% | 78.5% | (many diffs) |
| 61 | human | 818 | CAMK2G | calcium/calmodulin dependen... | XM_005270198.1 | 71.3% | 78.8% | (many diffs) |
| 62 | human | 816 | CAMK2B | calcium/calmodulin dependen... | XM_017012667.2 | 70.9% | 70.7% | 0_1ins387;560_632delinsC |
| 63 | human | 816 | CAMK2B | calcium/calmodulin dependen... | XM_011515547.1 | 70.8% | 70.8% | 947_1019delinsC;1131_1175del;1224_1724del |
| 64 | human | 818 | CAMK2G | calcium/calmodulin dependen... | NM_001367548.1 | 70.2% | 76.9% | (many diffs) |
| 65 | human | 818 | CAMK2G | calcium/calmodulin dependen... | NM_001367544.1 | 70.1% | 77.5% | (many diffs) |
| 66 | human | 818 | CAMK2G | calcium/calmodulin dependen... | NM_001367534.1 | 69% | 75.7% | (many diffs) |
| 67 | human | 816 | CAMK2B | calcium/calmodulin dependen... | XM_017012666.2 | 68.9% | 68.8% | 0_1ins387;560_632delinsC;744_788del |
| 68 | human | 816 | CAMK2B | calcium/calmodulin dependen... | XM_017012669.2 | 67.8% | 66.8% | (many diffs) |
| 69 | human | 818 | CAMK2G | calcium/calmodulin dependen... | XM_024448219.1 | 65.4% | 71.2% | (many diffs) |
| 70 | human | 818 | CAMK2G | calcium/calmodulin dependen... | NM_001367539.1 | 61.1% | 66.6% | (many diffs) |
| 71 | human | 818 | CAMK2G | calcium/calmodulin dependen... | NM_001367537.1 | 60.4% | 63% | (many diffs) |
| 72 | human | 816 | CAMK2B | calcium/calmodulin dependen... | XM_017012663.2 | 53.7% | 50.7% | (many diffs) |
| 73 | human | 816 | CAMK2B | calcium/calmodulin dependen... | XM_011515559.2 | 52.7% | 52.6% | (many diffs) |
| 74 | mouse | 12323 | Camk2b | calcium/calmodulin-dependen... | XM_006514479.2 | 92.1% | 99.2% | (many diffs) |
| 75 | mouse | 12323 | Camk2b | calcium/calmodulin-dependen... | XM_006514476.1 | 89.5% | 96.3% | (many diffs) |
| 76 | mouse | 12323 | Camk2b | calcium/calmodulin-dependen... | XM_006514477.1 | 89.3% | 96.1% | (many diffs) |
| 77 | mouse | 12323 | Camk2b | calcium/calmodulin-dependen... | XM_006514475.1 | 87.8% | 94.4% | (many diffs) |
| 78 | mouse | 12323 | Camk2b | calcium/calmodulin-dependen... | XM_017314240.1 | 87.7% | 94.5% | (many diffs) |
| 79 | mouse | 12323 | Camk2b | calcium/calmodulin-dependen... | NM_001174054.1 | 87.6% | 94.3% | (many diffs) |
| 80 | mouse | 12323 | Camk2b | calcium/calmodulin-dependen... | NM_007595.5 | 85.4% | 91.8% | (many diffs) |
| 81 | mouse | 12323 | Camk2b | calcium/calmodulin-dependen... | XM_017314239.1 | 85.3% | 91.8% | (many diffs) |
| 82 | mouse | 12323 | Camk2b | calcium/calmodulin-dependen... | NM_001174053.1 | 84.6% | 91.2% | (many diffs) |
| 83 | mouse | 12325 | Camk2g | calcium/calmodulin-dependen... | NM_001039139.2 | 76.8% | 87.9% | (many diffs) |
| 84 | mouse | 12325 | Camk2g | calcium/calmodulin-dependen... | NM_001039138.2 | 76.7% | 86.1% | (many diffs) |
| 85 | mouse | 12325 | Camk2g | calcium/calmodulin-dependen... | XM_017315827.1 | 76.1% | 87% | (many diffs) |
| 86 | mouse | 12322 | Camk2a | calcium/calmodulin-dependen... | XM_017317805.2 | 75.8% | 81.1% | (many diffs) |
| 87 | mouse | 12322 | Camk2a | calcium/calmodulin-dependen... | NM_177407.4 | 75.6% | 81.1% | (many diffs) |
| 88 | mouse | 12325 | Camk2g | calcium/calmodulin-dependen... | NM_178597.5 | 75.3% | 83.9% | (many diffs) |
| 89 | mouse | 12325 | Camk2g | calcium/calmodulin-dependen... | XM_006518497.2 | 74.5% | 81.9% | (many diffs) |
| 90 | mouse | 12323 | Camk2b | calcium/calmodulin-dependen... | XM_006514469.2 | 73.8% | 79.5% | (many diffs) |
| 91 | mouse | 12325 | Camk2g | calcium/calmodulin-dependen... | XM_017315826.1 | 73.8% | 84.4% | (many diffs) |
| 92 | mouse | 12325 | Camk2g | calcium/calmodulin-dependen... | XM_006518496.3 | 73.7% | 82.7% | (many diffs) |
| 93 | mouse | 12323 | Camk2b | calcium/calmodulin-dependen... | XM_006514470.3 | 73.7% | 79.4% | (many diffs) |
| 94 | mouse | 12325 | Camk2g | calcium/calmodulin-dependen... | XM_006518498.2 | 73.2% | 83.5% | (many diffs) |
| 95 | mouse | 12325 | Camk2g | calcium/calmodulin-dependen... | XM_006518494.2 | 72.5% | 80.7% | (many diffs) |
| 96 | mouse | 12323 | Camk2b | calcium/calmodulin-dependen... | XM_006514467.2 | 72.2% | 77.7% | (many diffs) |
| 97 | mouse | 12323 | Camk2b | calcium/calmodulin-dependen... | XM_017314238.1 | 72% | 77.5% | (many diffs) |
| 98 | mouse | 12325 | Camk2g | calcium/calmodulin-dependen... | XM_006518492.2 | 71.9% | 80.4% | (many diffs) |
| 99 | mouse | 12323 | Camk2b | calcium/calmodulin-dependen... | XM_017314237.1 | 71% | 76.5% | (many diffs) |
| 100 | mouse | 12325 | Camk2g | calcium/calmodulin-dependen... | XM_006518490.3 | 70.7% | 78.5% | (many diffs) |
| 101 | mouse | 12323 | Camk2b | calcium/calmodulin-dependen... | XM_006514465.2 | 69.5% | 74.7% | (many diffs) |
| 102 | mouse | 12323 | Camk2b | calcium/calmodulin-dependen... | XM_006514464.2 | 69.5% | 74.8% | (many diffs) |
| 103 | mouse | 12325 | Camk2g | calcium/calmodulin-dependen... | XM_006518489.2 | 69.3% | 77.5% | (many diffs) |
| 104 | mouse | 12325 | Camk2g | calcium/calmodulin-dependen... | XM_006518488.2 | 68.2% | 75.7% | (many diffs) |
| 105 | mouse | 12325 | Camk2g | calcium/calmodulin-dependen... | XM_006518500.3 | 57.5% | 62.4% | (many diffs) |
| 106 | mouse | 12323 | Camk2b | calcium/calmodulin-dependen... | XM_017314241.1 | 46.1% | 45.4% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1575
- ORF length:
- 1509
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggc caccacggtg acctgcaccc gcttcaccga cgagtaccag ctctacgagg 121 atattggcaa gggggctttc tctgtggtcc gacgctgtgt caagctctgc accggccatg 181 agtatgcagc caagatcatc aacaccaaga agctgtcagc cagagatcac cagaagctgg 241 agagagaggc tcggatctgc cgccttctga agcattccaa catcgtgcgt ctccacgaca 301 gcatctccga ggagggcttc cactacctgg tcttcgatct ggtcactggt ggggagctct 361 ttgaagacat tgtggcgaga gagtactaca gcgaggctga tgccagtcac tgtatccagc 421 agatcctgga ggccgttctc cattgtcacc aaatgggggt cgtccacaga gacctcaagc 481 cggagaacct gcttctggcc agcaagtgca aaggggctgc agtgaagctg gcagacttcg 541 gcctagctat cgaggtgcag ggggaccagc aggcatggtt tggtttcgct ggcacaccag 601 gctacctgtc ccctgaggtc cttcgcaaag aggcgtatgg caagcctgtg gacatctggg 661 catgtggggt gatcctgtac atcctgctcg tgggctaccc acccttctgg gacgaggacc 721 agcacaagct gtaccagcag atcaaggctg gtgcctatga cttcccgtcc cctgagtggg 781 acaccgtcac tcctgaagcc aaaaacctca tcaaccagat gctgaccatc aaccctgcca 841 agcgcatcac agcccatgag gccctgaagc acccgtgggt ctgccaacgc tccacggtag 901 catccatgat gcacagacag gagactgtgg agtgtctgaa aaagttcaat gccaggagaa 961 agctcaaggg agccatcctc accaccatgc tggccacacg gaatttctca gcagccaaga 1021 gtttactcaa caagaaagca gatggagtca agccccagac gaatagcacc aaaaacagtg 1081 cagccgccac cagccccaaa gggacgcttc ctcctgccgc cctggagtct tctgacagtg 1141 ccaataccac catagaggat gaagacgcta aagcccggaa gcaggagatc attaagacca 1201 cggagcagct catcgaggcc gtcaacaacg gtgactttga ggccTACGCG AAAATCTGTG 1261 ACCCAGGGCT GACCTCGTTT GAGCCTGAAG CACTGGGCAA CCTGGTTGAA GGGATGGACT 1321 TCCACAGATT CTACTTCGAG AACCTGCTGG CCAAGAACAG CAAGCCGATC CACACGACCA 1381 TCCTGAACCC ACACGTGCAC GTCATTGGAG AGGATGCCGC CTGCATCGCT TACATCCGGC 1441 TCACGCAGTA CATTGACGGG CAGGGCCGGC CCCGCACCAG CCAGTCTGAG GAGACCCGCG 1501 TGTGGCACCG CCGCGACGGC AAGTGGCAGA ACGTGCACTT CCACTGCTCG GGCGCGCCTG 1561 TGGCCCCGCT GCAGTGCCCA ACTTTCTTGT ACAAAGTGGT TGATATCGGT AAGCCTATCC 1621 CTAACCCTCT CCTCGGTCTC GATTCTACGT AGTAATGAAC TAGTCCGTAA CTTGAAAGTA 1681 TTTCGATTTC TTGGCTTTAT ATATCTTGTG GAAAGGACGA GACAGTGGGG GTGATCTCGG 1741 AGCGACGCGT TAAGTCgaca atcaacctct ggattacaaa atttgtgaaa gatt