Construct: ORF TRCN0000489808

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF020660.1_s317c1
DNA Barcode:
GCGAATCGTGCGTGGGCGGTAGCG
Epitope Tag:
V5 (not translated due to prior stop codon)
Notes:
Early stop codon detected

Originally Annotated References:

Gene:
EPHA6 (285220)

Vector Information:

Vector Backbone:
pLX_317
Pol II Cassette 1:
SV40-PuroR
Pol II Cassette 2:
EF1a-TRCN0000489808
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
n/a

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 285220 EPHA6 EPH receptor A6 NM_173655.4 95.7% 100% 1002_1003ins44
2 human 285220 EPHA6 EPH receptor A6 XM_017006223.1 84.5% 81.4% (many diffs)
3 human 285220 EPHA6 EPH receptor A6 NM_001278300.2 80.9% 83.9% 961_1152del;1194_1195ins44
4 human 285220 EPHA6 EPH receptor A6 XM_017006220.1 65.2% 61.6% (many diffs)
5 human 285220 EPHA6 EPH receptor A6 XM_017006217.1 60.3% 54.6% (many diffs)
6 human 285220 EPHA6 EPH receptor A6 XM_017006218.1 60.3% 54.6% (many diffs)
7 human 285220 EPHA6 EPH receptor A6 XM_017006216.1 57.8% 52.4% (many diffs)
8 human 285220 EPHA6 EPH receptor A6 XM_017006215.1 56.3% 51% (many diffs)
9 human 285220 EPHA6 EPH receptor A6 XM_017006214.1 42.7% 38.6% (many diffs)
10 human 285220 EPHA6 EPH receptor A6 XM_017006213.1 33.8% 30.6% (many diffs)
11 human 285220 EPHA6 EPH receptor A6 XM_017006212.1 33% 29.9% (many diffs)
12 human 285220 EPHA6 EPH receptor A6 XM_017006211.1 32.8% 29.7% (many diffs)
13 human 285220 EPHA6 EPH receptor A6 NM_001080448.3 29.6% 26.8% (many diffs)
14 human 285220 EPHA6 EPH receptor A6 XM_006713592.3 29.2% 26.4% (many diffs)
15 human 285220 EPHA6 EPH receptor A6 XM_017006210.1 25.5% 22.6% (many diffs)
16 human 285220 EPHA6 EPH receptor A6 XR_001740110.1 7.6% (many diffs)
17 mouse 13840 Epha6 Eph receptor A6 XM_006521787.2 30% 30.5% (many diffs)
18 mouse 13840 Epha6 Eph receptor A6 NM_007938.2 25.9% 26.3% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
99
ORF end:
1101
ORF length:
1002
Sequence:
1tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag
61caggctccgc ggccgccccc ttcaccctcg agcaggagat gaaagactct ccatttcaag
121tgacaaaact gtactggctt aatgaaaagt gggactttat tgcctcagct tctgacatgg
181cagcagaaca aggacagatt ctcgtgatag ccaccgccgc tgttggcgga ttcactctcc
241tcgtcatcct cactttattc ttcttgatca ctgggagatg tcagtggtac ataaaagcca
301agatgaagtc agaagagaag agaagaaacc acttacagaa tgggcatttg cgcttcccgg
361gaattaaaac ttacattgat ccagatacat atgaagaccc atccctagca gtccatgaat
421ttgcaaagga gattgatccc tcaagaattc gtattgagag agtcattggg gcaggtgaat
481ttggagaagt ctgtagtggg cgtttgaaga caccagggaa aagagagatc ccagttgcca
541ttaaaacttt gaaaggtggc cacatggatc ggcaaagaag agattttcta agagaagcta
601gtatcatggg ccagtttgac catccaaaca tcattcgcct agaaggggtt gtcaccaaaa
661gatccttccc ggccattggg gtggaggcgt tttgccccag cttcctgagg gcagggtttt
721taaatagcat ccaggccccg catccagtgc cagggggagg atctttgccc cccaggattc
781ctgctggcag aCCAGTAATG ATTGTGGTGG AATATATGGA GAATGGATCC CTAGACTCCT
841TTTTGCGGAA GCATGATGGC CACTTCACAG TCATCCAGTT GGTCGGAATG CTCCGAGGCA
901TTGCATCAGG CATGAAGTAT CTTTCTGATA TGGGTTATGT TCATCGAGAC CTAGCGGCTC
961GGAATATACT GGTCAATAGC AACTTAGTAT GCAAAGTTTC TGATTTTGGT CTCTCCAGAG
1021TGCTGGAAGA TGATCCAGAA GCTGCTTATA CAACAACTGA CCTCTTCCAA ACTCTAACAC
1081TTAACCTCTG CTATTCTGCA TAAATTCTGA GAAAAGCCAA ATTTTCTGTC GGTCTAAGAA
1141GACATAGCCT ACACCGGTAC CAAGGGTGGG CGCGCCGACC CAGCTTTCTT GTACAAAGTG
1201GTTGATATCG GTAAGCCTAT CCCTAACCCT CTCCTCGGTC TCGATTCTAC GTAGTAATGA
1261ACTAGTCCGT AACTTGAAAG TATTTCGATT TCTTGGCTTT ATATATCTTG TGGAAAGGAC
1321GAGCGAATCG TGCGTGGGCG GTAGCGACGC GTTAAGTCga caatcaacct ctggattaca
1381aaatttgtga aagatt

Download FASTA (ORF) (Full)

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