Transcript: Mouse XM_030254516.1

PREDICTED: Mus musculus mitogen-activated protein kinase 10 (Mapk10), transcript variant X1, mRNA.

Source:
NCBI, updated 2019-08-08
Taxon:
Mus musculus (mouse)
Gene:
Mapk10 (26414)
Length:
8133
CDS:
2130..3524

Additional Resources:

NCBI RefSeq record:
XM_030254516.1
NBCI Gene record:
Mapk10 (26414)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Mouse XM_030254516.1, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Mouse Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000360388 GCGGATTCTGAGCACAATAAA pLKO_005 3087 CDS 100% 15.000 21.000 N Mapk10 n/a
2 TRCN0000360389 GTATGTGGTGACGCGATATTA pLKO_005 2795 CDS 100% 15.000 21.000 N Mapk10 n/a
3 TRCN0000194979 CCATTTCATGTGATCTATTAC pLKO.1 4144 3UTR 100% 13.200 18.480 N MAPK10 n/a
4 TRCN0000012633 GCGATAGAACACAGCACACAT pLKO.1 3568 3UTR 100% 4.950 6.930 N Mapk10 n/a
5 TRCN0000201207 GTTGGAACCAAGTCAACGTAT pLKO.1 8070 3UTR 100% 4.950 6.930 N Mapk10 n/a
6 TRCN0000360315 CAATAGAGAGATCCAACATAA pLKO_005 3725 3UTR 100% 13.200 10.560 N Mapk10 n/a
7 TRCN0000417287 ATGAAGTGTGTGAACCATAAA pLKO_005 2472 CDS 100% 13.200 9.240 N MAPK10 n/a
8 TRCN0000191064 CCAAGTCAACGTATTGTAATT pLKO.1 8077 3UTR 100% 13.200 9.240 N Mapk10 n/a
9 TRCN0000012634 CGAAGAATGGAAAGAACTTAT pLKO.1 3290 CDS 100% 13.200 9.240 N Mapk10 n/a
10 TRCN0000216662 CTCAACCTTCACCGTTCTTAA pLKO.1 2294 CDS 100% 13.200 9.240 N Mapk10 n/a
11 TRCN0000196370 GACAGAAATGTGGCCATTAAG pLKO.1 2388 CDS 100% 13.200 9.240 N MAPK10 n/a
12 TRCN0000012636 GTGTCATCTATTGCCAAACAT pLKO.1 2217 CDS 100% 5.625 3.938 N Mapk10 n/a
13 TRCN0000012637 CAAGTCTGATTGCACACTGAA pLKO.1 2720 CDS 100% 4.950 3.465 N Mapk10 n/a
14 TRCN0000012635 CCAAGATGTCTACTTAGTGAT pLKO.1 2546 CDS 100% 4.950 3.465 N Mapk10 n/a
15 TRCN0000432277 GCCTAGTCAGATGGATGTAGA pLKO_005 4024 3UTR 100% 4.950 3.465 N MAPK10 n/a
16 TRCN0000191558 GAATCATTAAAGCTGAAGGAA pLKO.1 8110 3UTR 100% 3.000 2.100 N Mapk10 n/a
17 TRCN0000001020 GCCATTAAGAAGCTCAGCAGA pLKO.1 2400 CDS 100% 2.640 1.848 N MAPK10 n/a
18 TRCN0000001938 GCCATTAAGAAGCTCAGCAGA pLKO.1 2400 CDS 100% 2.640 1.848 N MAPK10 n/a
19 TRCN0000166364 CACACACACACACACACACAA pLKO.1 526 5UTR 100% 4.950 2.475 Y KAAG1 n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript XM_030254516.1, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 TRCN0000487812 TGCAGATTATTTTACTCCTCGACG pLX_317 20.5% 91.4% 89.4% V5 (not translated due to prior stop codon) (many diffs) n/a
2 TRCN0000488648 ACCAGGGTGAGTGAATCTAAGAAG pLX_317 20.3% 91.3% 89.4% V5 (not translated due to prior stop codon) (many diffs) n/a
3 TRCN0000492117 CTAAGCTAACTACGGCGGTACGCC pLX_317 31.1% 82.3% 89.4% V5 (many diffs) n/a
4 TRCN0000491457 CCGTCCACGCTTTTACTCGGCAGT pLX_317 24.4% 82.3% 89.4% V5 (not translated due to prior stop codon) (many diffs) n/a
5 TRCN0000489338 CTCATGACGTTAAATTTCAGTTAA pLX_317 28.4% 63.9% 75.4% V5 (many diffs) n/a
6 TRCN0000489471 GTGAGACGATACCTATTGCCACGT pLX_317 32.6% 63.9% 75.4% V5 (not translated due to prior stop codon) (many diffs) n/a
7 TRCN0000492156 AACAGCCTCTTGTTACGAGGTCTG pLX_317 19.2% 63.9% 75.4% V5 (not translated due to prior stop codon) (many diffs) n/a
8 ccsbBroadEn_11057 pDONR223 100% 62.4% 68.7% None (many diffs) n/a
9 ccsbBroad304_11057 pLX_304 0% 62.4% 68.7% V5 (many diffs) n/a
10 TRCN0000468220 CCCGCTATCGAACCGGGCATATAA pLX_317 45% 62.4% 68.7% V5 (many diffs) n/a
11 ccsbBroadEn_14805 pDONR223 0% 62.4% 68.7% None (many diffs) n/a
12 ccsbBroad304_14805 pLX_304 0% 62.4% 68.7% V5 (many diffs) n/a
13 TRCN0000466133 TCATAAGCAAACAGGTTCCCACAG pLX_317 29.1% 62.4% 68.7% V5 (many diffs) n/a
14 ccsbBroadEn_11058 pDONR223 100% 60.8% 67% None (many diffs) n/a
15 ccsbBroad304_11058 pLX_304 0% 60.8% 67% V5 (many diffs) n/a
16 TRCN0000475774 GTGAGTTGCCAATCATGCAATTTA pLX_317 29% 60.8% 67% V5 (many diffs) n/a
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