Construct: ORF ccsbBroad304_10876

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF011473.1_s304c1, BRDN0000403392
Derived from:
ccsbBroadEn_10876
DNA Barcode:
None
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
HLA-C (3107)

Vector Information:

Vector Backbone:
pLX_304
Pol II Cassette 1:
PGK-BlastR
Pol II Cassette 2:
CMV-ccsbBroad304_10876
Selection Marker:
BlastR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 3107 HLA-C major histocompatibility co... NM_001243042.1 96.6% 93.1% (many diffs)
2 human 3107 HLA-C major histocompatibility co... NM_002117.6 96.6% 93.1% (many diffs)
3 human 3106 HLA-B major histocompatibility co... NM_005514.8 92.4% 88.2% (many diffs)
4 human 3105 HLA-A major histocompatibility co... NM_002116.8 89.1% 84.1% (many diffs)
5 human 3105 HLA-A major histocompatibility co... NM_001242758.1 88.6% 82.5% (many diffs)
6 human 3133 HLA-E major histocompatibility co... NM_005516.6 84.9% 75.6% (many diffs)
7 human 3133 HLA-E major histocompatibility co... XM_017010809.2 83.7% 72.4% (many diffs)
8 human 3135 HLA-G major histocompatibility co... NM_002127.5 82.5% 76.7% (many diffs)
9 human 3135 HLA-G major histocompatibility co... XM_024446420.1 82.5% 76.7% (many diffs)
10 human 3134 HLA-F major histocompatibility co... NM_018950.2 81.6% 75.6% (many diffs)
11 human 3135 HLA-G major histocompatibility co... NM_001363567.1 81.4% 75.7% (many diffs)
12 human 3134 HLA-F major histocompatibility co... XM_011514564.1 80.5% 74.5% (many diffs)
13 human 3134 HLA-F major histocompatibility co... XM_017010813.1 78.2% 70.8% (many diffs)
14 human 3133 HLA-E major histocompatibility co... XM_017010807.1 75.9% 62.4% (many diffs)
15 human 3133 HLA-E major histocompatibility co... XM_017010808.1 75.9% 62.4% (many diffs)
16 human 3134 HLA-F major histocompatibility co... XM_017010812.1 72.4% 65.4% (many diffs)
17 human 3134 HLA-F major histocompatibility co... XM_017010811.1 71.5% 62.3% (many diffs)
18 human 3134 HLA-F major histocompatibility co... XM_017010810.1 70% 63.2% (many diffs)
19 human 3134 HLA-F major histocompatibility co... NM_001098479.2 68.9% 62.2% (many diffs)
20 human 3135 HLA-G major histocompatibility co... XM_017010817.1 59.9% 55.4% (many diffs)
21 human 3135 HLA-G major histocompatibility co... XM_017010818.1 59.9% 55.4% (many diffs)
22 human 3134 HLA-F major histocompatibility co... NM_001098478.2 59.3% 53.2% (many diffs)
23 human 3136 HLA-H major histocompatibility co... NR_001434.4 57.7% (many diffs)
24 human 3134 HLA-F major histocompatibility co... XM_017010815.1 54.3% 50.5% (many diffs)
25 human 3134 HLA-F major histocompatibility co... XM_017010814.1 46.5% 41.7% (many diffs)
26 human 3134 HLA-F major histocompatibility co... XR_001743373.1 29% (many diffs)
27 human 3134 HLA-F major histocompatibility co... XR_001743374.1 27.9% (many diffs)
28 human 3134 HLA-F major histocompatibility co... XR_001743376.1 27.8% (many diffs)
29 human 3139 HLA-L major histocompatibility co... NR_027822.1 16.1% (many diffs)
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Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
66
ORF end:
1164
ORF length:
1098
Sequence:
1ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag
61ttggcatgcg ggtcatggcg ccccgaaccc tcatcctgct gctctcggga gccctggccc
121tgaccgagac ctgggcctgc tcccactcca tgaggtattt ctacaccgcc gtgtcccggc
181ccggccgcgg agagccccgc ttcatcgcag tgggctacgt ggacgacacg cagttcgtgc
241ggttcgacag cgacgccgcg agtccaagag gggagccgcg ggcgccgtgg gtggagcagg
301aggggccgga gtattgggac cgggagacac agaagtacaa gcgccaggca cagactgacc
361gagtgagcct gcggaacctg cgcggctact acaaccagag cgaggccggg tctcacaccc
421tccagtggat gtatggctgc gacctggggc ccgacgggcg cctcctccgc gggtatgacc
481agtccgccta cgacggcaag gattacatcg ccctgaacga ggacctgcgc tcctggaccg
541ccgcggacac ggcggctcag atcacccagc gcaagtggga ggcggcccgt gcggcggagc
601agcagagagc ctacctggag ggcacgtgcg tggagtggct ccgcagatac ctggagaacg
661ggaaggagac gctgcagcgc gcggaacacc caaagacaca cgtgacccac catctcgtct
721ctgaccatga ggccaccctg aggtgctggg ccctgggctt ctaccctgcg gagatcacac
781tgacctggca gcgggatggc gaggaccaaa ctcaggacac cgagcttgtg gagaccaggc
841cagcaggaga tggaaccttc cagaagtggg cagctgtggt ggtgccttct ggagaagagc
901agagatacac gtgccatgtg cagcacgagg ggctgccgga gcccctcacc ctgagatggg
961agccatcttc ccagcccacc atccccatcg tgggcatcgt tgctggcctg gctgtcctgg
1021ctgtcctagc tgtcctagga gctgtggtgg ctgttgttat gtgtaggagg aagagctcag
1081gtggaaaagg agggagctgc tctcaggctg cgtccagcaa cagtgcccag ggctctgatg
1141agtctctcat cgcttgtaaa gcctgcccaa ctttcttgta caaagtggtt ggtaagccta
1201tccctaaccc tctcctcggt ctcgattcta cgtagtaatg agctagcgct aaccggtggc
1261gcgttaagtc gacaatcaac ctctggatta

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