Construct: ORF TRCN0000476436
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF016274.1_s317c1
- Derived from:
- ccsbBroadEn_02188
- DNA Barcode:
- TCCGTGTTGGTCAAGCGACGCGCC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- ZNF254 (9534)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000476436
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
---|---|---|---|---|---|---|---|---|
1 | human | 9534 | ZNF254 | zinc finger protein 254 | NM_203282.4 | 100% | 100% | |
2 | human | 9534 | ZNF254 | zinc finger protein 254 | NM_001278661.1 | 93.7% | 93.7% | 0_1ins123 |
3 | human | 9534 | ZNF254 | zinc finger protein 254 | NM_001278662.2 | 93.7% | 93.7% | 0_1ins123 |
4 | human | 9534 | ZNF254 | zinc finger protein 254 | NM_001278677.2 | 93.7% | 93.7% | 0_1ins123 |
5 | human | 9534 | ZNF254 | zinc finger protein 254 | XM_011528443.1 | 93.7% | 93.7% | 0_1ins123 |
6 | human | 9534 | ZNF254 | zinc finger protein 254 | XM_011528444.2 | 93.7% | 93.7% | 0_1ins123 |
7 | human | 9534 | ZNF254 | zinc finger protein 254 | XM_017027515.1 | 93.7% | 93.7% | 0_1ins123 |
8 | human | 9534 | ZNF254 | zinc finger protein 254 | XM_017027517.1 | 89.4% | 87.8% | (many diffs) |
9 | human | 9534 | ZNF254 | zinc finger protein 254 | NM_001278664.1 | 88.9% | 88.9% | 0_1ins219 |
10 | human | 9534 | ZNF254 | zinc finger protein 254 | XM_017027518.1 | 88.9% | 88.9% | 0_1ins219 |
11 | human | 9534 | ZNF254 | zinc finger protein 254 | XM_017027519.1 | 88.9% | 88.9% | 0_1ins219 |
12 | human | 9534 | ZNF254 | zinc finger protein 254 | NM_001278663.2 | 87.1% | 87.1% | 0_1ins255 |
13 | human | 9534 | ZNF254 | zinc finger protein 254 | NM_001278678.1 | 87.1% | 87.1% | 0_1ins255 |
14 | human | 9534 | ZNF254 | zinc finger protein 254 | XM_011528448.3 | 87.1% | 87.1% | 0_1ins255 |
15 | human | 730087 | ZNF726 | zinc finger protein 726 | NM_001244038.2 | 82% | 75.7% | (many diffs) |
16 | human | 7639 | ZNF85 | zinc finger protein 85 | NM_003429.5 | 76.5% | 67.2% | (many diffs) |
17 | human | 7639 | ZNF85 | zinc finger protein 85 | NM_001256171.1 | 73.2% | 64.4% | (many diffs) |
18 | human | 148198 | ZNF98 | zinc finger protein 98 | NM_001098626.2 | 73% | 65.5% | (many diffs) |
19 | human | 7562 | ZNF708 | zinc finger protein 708 | NM_021269.3 | 72.9% | 64.3% | (many diffs) |
20 | human | 113835 | ZNF257 | zinc finger protein 257 | NM_033468.4 | 72.1% | 62.8% | (many diffs) |
21 | human | 7562 | ZNF708 | zinc finger protein 708 | XM_017027201.1 | 72.1% | 63.5% | (many diffs) |
22 | human | 7639 | ZNF85 | zinc finger protein 85 | XM_011528263.2 | 71.6% | 62.6% | (many diffs) |
23 | human | 170959 | ZNF431 | zinc finger protein 431 | NM_133473.4 | 70.1% | 59.8% | (many diffs) |
24 | human | 170959 | ZNF431 | zinc finger protein 431 | NM_001319124.2 | 70% | 59.7% | (many diffs) |
25 | human | 80264 | ZNF430 | zinc finger protein 430 | NM_001172671.2 | 69.3% | 60.2% | (many diffs) |
26 | human | 80264 | ZNF430 | zinc finger protein 430 | NM_025189.4 | 69.2% | 60.1% | (many diffs) |
27 | human | 7594 | ZNF43 | zinc finger protein 43 | NM_001256653.1 | 68.9% | 58.7% | (many diffs) |
28 | human | 7594 | ZNF43 | zinc finger protein 43 | NM_003423.4 | 68.1% | 58% | (many diffs) |
29 | human | 170959 | ZNF431 | zinc finger protein 431 | XM_011527750.2 | 67.9% | 58.4% | (many diffs) |
30 | human | 10793 | ZNF273 | zinc finger protein 273 | NM_021148.2 | 67.8% | 59% | (many diffs) |
31 | human | 7594 | ZNF43 | zinc finger protein 43 | NM_001256648.2 | 67.4% | 57.4% | (many diffs) |
32 | human | 7594 | ZNF43 | zinc finger protein 43 | NM_001256649.2 | 67.4% | 57.4% | (many diffs) |
33 | human | 7594 | ZNF43 | zinc finger protein 43 | NM_001256650.2 | 67.4% | 57.4% | (many diffs) |
34 | human | 7594 | ZNF43 | zinc finger protein 43 | XM_011528257.3 | 67.4% | 57.4% | (many diffs) |
35 | human | 7594 | ZNF43 | zinc finger protein 43 | XM_011528259.3 | 67.4% | 57.4% | (many diffs) |
36 | human | 7594 | ZNF43 | zinc finger protein 43 | XM_017027207.2 | 67.4% | 57.4% | (many diffs) |
37 | human | 7594 | ZNF43 | zinc finger protein 43 | XM_017027208.2 | 67.4% | 57.4% | (many diffs) |
38 | human | 7594 | ZNF43 | zinc finger protein 43 | XM_017027209.2 | 67.4% | 57.4% | (many diffs) |
39 | human | 7594 | ZNF43 | zinc finger protein 43 | XM_017027210.2 | 67.4% | 57.4% | (many diffs) |
40 | human | 7594 | ZNF43 | zinc finger protein 43 | XM_017027211.2 | 67.4% | 57.4% | (many diffs) |
41 | human | 7594 | ZNF43 | zinc finger protein 43 | XM_017027212.2 | 67.4% | 57.4% | (many diffs) |
42 | human | 7594 | ZNF43 | zinc finger protein 43 | XM_017027213.2 | 67.4% | 57.4% | (many diffs) |
43 | human | 7594 | ZNF43 | zinc finger protein 43 | XM_017027214.2 | 67.4% | 57.4% | (many diffs) |
44 | human | 7594 | ZNF43 | zinc finger protein 43 | XM_017027215.2 | 67.4% | 57.4% | (many diffs) |
45 | human | 7594 | ZNF43 | zinc finger protein 43 | XM_017027216.2 | 67.4% | 57.4% | (many diffs) |
46 | human | 57615 | ZNF492 | zinc finger protein 492 | NM_020855.3 | 67.4% | 60.2% | (many diffs) |
47 | human | 163227 | ZNF100 | zinc finger protein 100 | NM_001351669.2 | 65.2% | 57.2% | (many diffs) |
48 | human | 163227 | ZNF100 | zinc finger protein 100 | NM_173531.4 | 65.1% | 57.1% | (many diffs) |
49 | human | 170959 | ZNF431 | zinc finger protein 431 | NM_001319126.2 | 61.9% | 52.4% | (many diffs) |
50 | human | 170959 | ZNF431 | zinc finger protein 431 | NM_001319127.2 | 61.2% | 52.1% | (many diffs) |
51 | human | 440515 | ZNF506 | zinc finger protein 506 | NM_001145404.2 | 53.5% | 43.3% | (many diffs) |
52 | human | 7644 | ZNF91 | zinc finger protein 91 | NM_003430.4 | 49% | 43.6% | (many diffs) |
53 | human | 7644 | ZNF91 | zinc finger protein 91 | NM_001300951.2 | 46.5% | 41.3% | (many diffs) |
54 | human | 7644 | ZNF91 | zinc finger protein 91 | XM_011528265.3 | 45.8% | 40.6% | (many diffs) |
55 | human | 7644 | ZNF91 | zinc finger protein 91 | XM_017027235.1 | 45.8% | 40.6% | (many diffs) |
56 | human | 7644 | ZNF91 | zinc finger protein 91 | XM_017027236.1 | 43.3% | 38.1% | (many diffs) |
57 | human | 388524 | RPSAP58 | ribosomal protein SA pseudo... | NR_149350.2 | 42.6% | (many diffs) | |
58 | human | 163227 | ZNF100 | zinc finger protein 100 | NM_001351672.2 | 42.5% | 35.9% | (many diffs) |
59 | human | 388524 | RPSAP58 | ribosomal protein SA pseudo... | NR_149351.2 | 41.3% | (many diffs) | |
60 | human | 7644 | ZNF91 | zinc finger protein 91 | XM_006722880.4 | 41.3% | 36.4% | (many diffs) |
61 | human | 7644 | ZNF91 | zinc finger protein 91 | XM_011528266.2 | 41.3% | 36.4% | (many diffs) |
62 | human | 7644 | ZNF91 | zinc finger protein 91 | XR_001753756.2 | 34.8% | (many diffs) | |
63 | human | 7644 | ZNF91 | zinc finger protein 91 | XR_001753755.2 | 25.6% | (many diffs) | |
64 | human | 7644 | ZNF91 | zinc finger protein 91 | XR_001753754.2 | 19.8% | (many diffs) | |
65 | human | 7644 | ZNF91 | zinc finger protein 91 | XM_024451693.1 | 15.5% | 12% | (many diffs) |
66 | human | 7644 | ZNF91 | zinc finger protein 91 | XR_002958354.1 | 14.5% | (many diffs) | |
67 | human | 730087 | ZNF726 | zinc finger protein 726 | NM_001348687.2 | 14.2% | 10.9% | (many diffs) |
68 | human | 9534 | ZNF254 | zinc finger protein 254 | NM_001278665.2 | 13.5% | 12.8% | (many diffs) |
69 | human | 730087 | ZNF726 | zinc finger protein 726 | NM_001348688.2 | 12.2% | 10.5% | (many diffs) |
70 | human | 7639 | ZNF85 | zinc finger protein 85 | NM_001256172.2 | 11.5% | 9.7% | (many diffs) |
71 | human | 730087 | ZNF726 | zinc finger protein 726 | NM_001348689.2 | 10.9% | 10.4% | (many diffs) |
72 | human | 440519 | ZNF724 | zinc finger protein 724 | XM_024451521.1 | 10.7% | 10.1% | (many diffs) |
73 | human | 51427 | ZNF107 | zinc finger protein 107 | XM_024446791.1 | 10% | 9.4% | (many diffs) |
74 | human | 7697 | ZNF138 | zinc finger protein 138 | NM_001271637.2 | 8.3% | 5.8% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 2046
- ORF length:
- 1977
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat gccaggaccc cctagaagcc tagaaatggg actgttgaca tttagggatg 121 tggccataga attctctctg gaggagtggc aacacctgga cattgcacag cagaatttat 181 atagaaatgt gatgttagag aactacagaa acctggcctt cctgggtatt gctgtctcta 241 agccagacct gatcacctgt ctggaacaag ggaaagagcc ctggaatatg aagcgacatg 301 agatggtgga tgaaccccca ggtatgtgtc ctcattttgc tcaagacctt tggccagagc 361 agggcatgga agattctttt caaaaagcaa tactgagaag atatggaaaa tatggacatg 421 agaatttaca gttaagaaaa ggctgtaaaa gtgtggatga gtataaggtg aacaaagaag 481 gttataatgg acttaaccag tgtttcacaa ctgcccagag caaagtattt caatgtgata 541 aatatttgaa agtcttctat aaatttttaa attcaaacag acctaagata agacatactg 601 aaaagaaatc tttcaaatgt aaaaaacgtg tcaaattatt ttgcatgctt tcacataaaa 661 cccaacacaa aagcatttat catagagaga agtcctacaa atgtaaagaa tgtggaaaaa 721 cctttaattg gtcctcaacc cttactaatc ataggaaaat ttatactgaa gagaaacctt 781 acaaatgtga agaatataac aaatctccta agcaactctc aacccttact acacatgaaa 841 taattcatgc tggagagaaa ctctacaaat gtgaagaatg tggtgaagct tttaatcgat 901 cctcaaatct tactacacat aagataattc atactggaga gaaaccttac aagtgtgaag 961 aatgtggcaa agcatttatc tggtcctcaa cccttactga gcataagaaa attcatacta 1021 gaaagaaacc ctacaagtgt gaagaatgtg gcaaagcatt tatatggtcc tcaacactaa 1081 ctagacataa gaggatgcac actggagaga aaccctacaa atgtgaagaa tgtggcaaag 1141 cttttagcca gtcctcaacc cttactacac ataagataat tcatactgga gagaaacgct 1201 acaaatgctt agaatgtggc aaagctttta agcaactctc aactcttact acacataaaa 1261 taattcatgt tggagagaaa ctctacaaat gtgaagaatg cggcaaaggt tttaatcgat 1321 cttcaaatct tactacacat aagataattc atactggaga gaaaccttac aagtgtgaag 1381 aatgtggcaa agcatttatc tggtcctcaa cccttactaa acataagaga attcatacta 1441 gagagaaacc ctacaaatgt gaagaatgtg gcaaggcatt tatatggtcc tcaaccctaa 1501 ctagacataa gaggatgcac actggagaga aaccctacaa atgtgaagaa tgtggcaaat 1561 cttttagcca atcctcaacc cttactacac ataagataat tcatactgga gagaaaccct 1621 acaaatgtga agaatgtggc aaagccttta actggtcctc aactcttact aaacataaga 1681 taattcatac tgaagagaaa ccctacaaat gtgaaaaatg tggcaaagcc tttaagcagt 1741 cttcaatcct tactaaccat aagagaattc atactggaga gaaaccctat aaatgtgaag 1801 aatgtgGCAA ATCTTTTAAC CGGTCTTCAA CTTTTACTAA ACATAAGGTA ATTCATACTG 1861 GAGTAAAACC CTACAAATGT GAAGAATGTG GCAAAGCATT TTTCTGGTCC TCAACCCTAA 1921 CTAAACATAA GAGAATTCAT ACTGGAGAGC AACCCTACAA ATGGGAAAAA TTTGGCAAAG 1981 CCTTTAATCG GTCCTCGCAC CTCACCACAG ATAAGATAAC TCATTGGAGA GAAATCTTAC 2041 AAGTATTGCC AACTTTCTTG TACAAAGTGG TTGATATCGG TAAGCCTATC CCTAACCCTC 2101 TCCTCGGTCT CGATTCTACG TAGTAATGAA CTAGTCCGTA ACTTGAAAGT ATTTCGATTT 2161 CTTGGCTTTA TATATCTTGT GGAAAGGACG ATCCGTGTTG GTCAAGCGAC GCGCCACGCG 2221 TTAAGTCgac aatcaacctc tggattacaa aatttgtgaa agatt