Transcript: Mouse NM_178203.2

Mus musculus histone cluster 1, H3b (Hist1h3b), mRNA.

Source:
NCBI, updated 2017-04-15
Taxon:
Mus musculus (mouse)
Gene:
Hist1h3b (319150)
Length:
461
CDS:
1..411

Additional Resources:

NCBI RefSeq record:
NM_178203.2
NBCI Gene record:
Hist1h3b (319150)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Mouse NM_178203.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Mouse Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000238059 CCTACCTTGTGGGTCTGTTTG pLKO_005 296 CDS 100% 10.800 5.400 Y Hist1h3f n/a
2 TRCN0000244235 AGATCGCGCAGGACTTCAAGA pLKO_005 221 CDS 100% 4.950 2.475 Y Hist1h3f n/a
3 TRCN0000093018 CTACCTTGTGGGTCTGTTTGA pLKO.1 297 CDS 100% 4.950 2.475 Y Hist1h3c n/a
4 TRCN0000092788 GCCTACCTTGTGGGTCTGTTT pLKO.1 295 CDS 100% 4.950 2.475 Y Hist1h3a n/a
5 TRCN0000238060 ACCATCATGCCCAAGGACATC pLKO_005 355 CDS 100% 4.050 2.025 Y Hist1h3f n/a
6 TRCN0000092826 CACCATCATGCCCAAGGACAT pLKO.1 354 CDS 100% 4.050 2.025 Y Hist1h3g n/a
7 TRCN0000238061 GGTCTGTTTGAGGACACCAAC pLKO_005 307 CDS 100% 4.050 2.025 Y Hist1h3f n/a
8 TRCN0000093022 CCTTGTGGGTCTGTTTGAGGA pLKO.1 300 CDS 100% 2.640 1.320 Y Hist1h3c n/a
9 TRCN0000429290 GTTTGAGGACACCAACCTGTG pLKO_005 312 CDS 100% 2.250 1.125 Y H3-4 n/a
10 TRCN0000092970 CGAGATCGCGCAGGACTTCAA pLKO.1 219 CDS 100% 1.650 0.825 Y Hist1h3e n/a
11 TRCN0000242124 GAGATCGCGCAGGACTTCAAG pLKO_005 220 CDS 100% 1.650 0.825 Y Hist1h3d n/a
12 TRCN0000092972 GAAGTCGACCGAGCTGCTGAT pLKO.1 168 CDS 100% 1.350 0.675 Y Hist1h3e n/a
13 TRCN0000248207 GATCGCGCAGGACTTCAAGAC pLKO_005 222 CDS 100% 1.350 0.675 Y Hist1h3b n/a
14 TRCN0000242125 AGAAGTCGACCGAGCTGCTGA pLKO_005 167 CDS 100% 0.880 0.440 Y Hist1h3d n/a
15 TRCN0000242127 GTGAAGAAGCCTCACCGCTAC pLKO_005 106 CDS 100% 0.750 0.375 Y Hist1h3d n/a
16 TRCN0000248204 TGAAGAAGCCTCACCGCTACC pLKO_005 107 CDS 100% 0.750 0.375 Y Hist1h3b n/a
17 TRCN0000242126 TTCAAGACCGACCTGCGCTTC pLKO_005 235 CDS 100% 0.750 0.375 Y Hist1h3d n/a
18 TRCN0000092971 CTACCAGAAGTCGACCGAGCT pLKO.1 162 CDS 100% 0.720 0.360 Y Hist1h3e n/a
19 TRCN0000092969 CTTCAAGACCGACCTGCGCTT pLKO.1 234 CDS 100% 0.720 0.360 Y Hist1h3e n/a
20 TRCN0000092808 GCAGGACTTCAAGACCGACCT pLKO.1 228 CDS 100% 0.720 0.360 Y Hist1h3h n/a
21 TRCN0000092789 GCGTGTCACCATCATGCCCAA pLKO.1 348 CDS 100% 0.720 0.360 Y Hist1h3a n/a
22 TRCN0000248205 ACTTCAAGACCGACCTGCGCT pLKO_005 233 CDS 100% 0.220 0.110 Y Hist1h3b n/a
23 TRCN0000262273 AGTCGACCGAGCTGCTGATCC pLKO_005 170 CDS 100% 0.000 0.000 Y Hist2h3b n/a
24 TRCN0000248206 ATCCGCAAGCTGCCGTTCCAG pLKO_005 187 CDS 100% 0.000 0.000 Y Hist1h3b n/a
25 TRCN0000092825 CCACGCCAAGCGTGTCACCAT pLKO.1 339 CDS 100% 0.000 0.000 Y Hist1h3g n/a
26 TRCN0000092968 CCAGAAGTCGACCGAGCTGCT pLKO.1 165 CDS 100% 0.000 0.000 Y Hist1h3e n/a
27 TRCN0000092809 CGAGCTGCTGATCCGCAAGCT pLKO.1 177 CDS 100% 0.000 0.000 Y Hist1h3h n/a
28 TRCN0000093021 CGAGGCCTACCTTGTGGGTCT pLKO.1 291 CDS 100% 0.000 0.000 Y Hist1h3c n/a
29 TRCN0000092842 CGCGCAGGACTTCAAGACCGA pLKO.1 225 CDS 100% 0.000 0.000 Y Hist1h3i n/a
30 TRCN0000092810 CTTCCAGAGCTCGGCCGTCAT pLKO.1 252 CDS 100% 0.000 0.000 Y Hist1h3h n/a
31 TRCN0000282160 GCGAGATCGCGCAGGACTTCA pLKO_005 218 CDS 100% 0.000 0.000 Y Hist2h3b n/a
32 TRCN0000092840 GCGCAAGCAGCTGGCCACCAA pLKO.1 51 CDS 100% 0.000 0.000 Y Hist1h3i n/a
33 TRCN0000092792 GCGCGAGATCGCGCAGGACTT pLKO.1 216 CDS 100% 0.000 0.000 Y Hist1h3a n/a
34 TRCN0000092812 GCGCTACCAGAAGTCGACCGA pLKO.1 159 CDS 100% 0.000 0.000 Y Hist1h3h n/a
35 TRCN0000092839 GCGTGAAGAAGCCTCACCGCT pLKO.1 104 CDS 100% 0.000 0.000 Y Hist1h3i n/a
36 TRCN0000092811 GCTGATCCGCAAGCTGCCGTT pLKO.1 183 CDS 100% 0.000 0.000 Y Hist1h3h n/a
37 TRCN0000257687 TACCAGAAGTCGACCGAGCTG pLKO_005 163 CDS 100% 0.000 0.000 Y Hist1h3b n/a
38 TRCN0000262272 TCCGCAAGCTGCCGTTCCAGC pLKO_005 188 CDS 100% 0.000 0.000 Y Hist2h3b n/a
39 TRCN0000106757 GCGAGATCGCGCAGGACTTTA pLKO.1 218 CDS 100% 4.400 2.200 Y H3C14 n/a
40 TRCN0000092885 CATGCCCAAGGACATCCAGTT pLKO.1 360 CDS 100% 4.050 2.025 Y Hist2h3c2 n/a
41 TRCN0000243830 CATGCCCAAGGACATCCAGTT pLKO_005 360 CDS 100% 4.050 2.025 Y H3C13 n/a
42 TRCN0000106788 GCAGGACTTCAAGACCGACTT pLKO.1 228 CDS 100% 4.050 2.025 Y H3C10 n/a
43 TRCN0000115681 CTGTTTGAGGACACCAACCTA pLKO.1 310 CDS 100% 3.000 1.500 Y H3C4 n/a
44 TRCN0000092887 CTGTGCGCCATCCACGCCAAA pLKO.1 328 CDS 100% 0.000 0.000 Y Hist2h3c2 n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript NM_178203.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_04820 pDONR223 100% 91.1% 100% None (many diffs) n/a
2 ccsbBroad304_04820 pLX_304 0% 91.1% 100% V5 (many diffs) n/a
3 TRCN0000470519 GCCGATTGAGCTCTTAAAAGGAAC pLX_317 100% 91.1% 100% V5 (many diffs) n/a
4 ccsbBroadEn_13992 pDONR223 100% 88.9% 7.5% None (many diffs) n/a
5 ccsbBroad304_13992 pLX_304 0% 88.9% 7.5% V5 (not translated due to prior stop codon) (many diffs) n/a
6 TRCN0000474854 TCCGGCCCACTCTACGTTTGCATT pLX_317 100% 88.9% 7.5% V5 (not translated due to prior stop codon) (many diffs) n/a
7 ccsbBroadEn_07225 pDONR223 100% 88.2% 98.5% None (many diffs) n/a
8 ccsbBroad304_07225 pLX_304 0% 88.2% 98.5% V5 (many diffs) n/a
9 ccsbBroadEn_07226 pDONR223 100% 88.2% 99.2% None (many diffs) n/a
10 ccsbBroad304_07226 pLX_304 0% 88.2% 99.2% V5 (many diffs) n/a
11 TRCN0000465999 CATGGCGATGGACTCTTGTAAGTC pLX_317 82.1% 88.2% 99.2% V5 (many diffs) n/a
12 ccsbBroadEn_01908 pDONR223 100% 88.2% 99.2% None (many diffs) n/a
13 ccsbBroad304_01908 pLX_304 0% 88.2% 99.2% V5 (many diffs) n/a
14 TRCN0000467485 CTCAATTAACCTTCGGAGAAGTTC pLX_317 89.4% 88.2% 99.2% V5 (many diffs) n/a
15 ccsbBroadEn_07214 pDONR223 100% 87.7% 96.3% None (many diffs) n/a
16 ccsbBroad304_07214 pLX_304 0% 87.7% 96.3% V5 (many diffs) n/a
17 TRCN0000477997 GCACAAGGTCGTGAATTCAGTTAA pLX_317 34.1% 87.7% 96.3% V5 (many diffs) n/a
18 ccsbBroadEn_01906 pDONR223 100% 87.5% 99.2% None (many diffs) n/a
19 ccsbBroad304_01906 pLX_304 0% 87.5% 99.2% V5 (many diffs) n/a
20 TRCN0000469791 ACACCATCTCGTTTATTTCAGCTA pLX_317 77.1% 87.5% 99.2% V5 (many diffs) n/a
21 ccsbBroadEn_01907 pDONR223 100% 87.2% 99.2% None (many diffs) n/a
22 ccsbBroad304_01907 pLX_304 0% 87.2% 99.2% V5 (many diffs) n/a
23 TRCN0000469614 CTGCCGATCGGACTGCTGGCTACG pLX_317 100% 87.2% 99.2% V5 (many diffs) n/a
24 ccsbBroadEn_07332 pDONR223 100% 87.2% 99.2% None (many diffs) n/a
25 ccsbBroad304_07332 pLX_304 0% 87.2% 99.2% V5 (many diffs) n/a
26 TRCN0000475169 CTGATGTGCTCCCTCCTTGCCCTC pLX_317 57.4% 87.2% 99.2% V5 (many diffs) n/a
27 ccsbBroadEn_01904 pDONR223 100% 87% 99.2% None (many diffs) n/a
28 ccsbBroad304_01904 pLX_304 97.8% 87% 99.2% V5 (many diffs) n/a
29 TRCN0000465966 TGCACCAAATCGTATAGACTTAAT pLX_317 74.7% 87% 99.2% V5 (many diffs) n/a
30 ccsbBroadEn_07333 pDONR223 100% 87% 99.2% None (many diffs) n/a
31 ccsbBroad304_07333 pLX_304 0% 87% 99.2% V5 (many diffs) n/a
32 TRCN0000468120 GCAGCACCCCACCATAATCTTTGA pLX_317 96% 87% 99.2% V5 (many diffs) n/a
33 ccsbBroadEn_02054 pDONR223 100% 86.5% 99.2% None (many diffs) n/a
34 ccsbBroad304_02054 pLX_304 0% 86.5% 99.2% V5 (many diffs) n/a
35 TRCN0000474049 ACGAGTAGAGTAGCATGTAAGCCT pLX_317 92% 86.5% 99.2% V5 (many diffs) n/a
36 ccsbBroadEn_01905 pDONR223 100% 86.5% 99.2% None (many diffs) n/a
37 ccsbBroad304_01905 pLX_304 0% 86.5% 99.2% V5 (many diffs) n/a
38 TRCN0000469866 TCGCGAGAACCCACGTCATTTGGC pLX_317 98.6% 86.5% 99.2% V5 (many diffs) n/a
39 ccsbBroadEn_01909 pDONR223 100% 86% 99.2% None (many diffs) n/a
40 ccsbBroad304_01909 pLX_304 97.2% 86% 99.2% V5 (many diffs) n/a
41 TRCN0000479054 GCGCGGCCAACGTGTTATAATTCG pLX_317 93.7% 86% 99.2% V5 (many diffs) n/a
42 ccsbBroadEn_00719 pDONR223 100% 82.8% 97% None (many diffs) n/a
43 ccsbBroad304_00719 pLX_304 0% 82.8% 97% V5 (many diffs) n/a
44 TRCN0000473475 ACAACCTAGAGAAGGGCCGCGTGA pLX_317 92% 82.8% 97% V5 (many diffs) n/a
45 ccsbBroadEn_10159 pDONR223 100% 80.6% 93.3% None (many diffs) n/a
46 ccsbBroad304_10159 pLX_304 0% 80.6% 93.3% V5 (many diffs) n/a
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