Transcript: Mouse NM_178215.2

Mus musculus histone cluster 2, H3b (Hist2h3b), mRNA.

Source:
NCBI, updated 2017-04-15
Taxon:
Mus musculus (mouse)
Gene:
Hist2h3b (319154)
Length:
502
CDS:
41..451

Additional Resources:

NCBI RefSeq record:
NM_178215.2
NBCI Gene record:
Hist2h3b (319154)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Mouse NM_178215.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Mouse Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000244235 AGATCGCGCAGGACTTCAAGA pLKO_005 261 CDS 100% 4.950 2.475 Y Hist1h3f n/a
2 TRCN0000238060 ACCATCATGCCCAAGGACATC pLKO_005 395 CDS 100% 4.050 2.025 Y Hist1h3f n/a
3 TRCN0000092826 CACCATCATGCCCAAGGACAT pLKO.1 394 CDS 100% 4.050 2.025 Y Hist1h3g n/a
4 TRCN0000092885 CATGCCCAAGGACATCCAGTT pLKO.1 400 CDS 100% 4.050 2.025 Y Hist2h3c2 n/a
5 TRCN0000243830 CATGCCCAAGGACATCCAGTT pLKO_005 400 CDS 100% 4.050 2.025 Y H3C13 n/a
6 TRCN0000092970 CGAGATCGCGCAGGACTTCAA pLKO.1 259 CDS 100% 1.650 0.825 Y Hist1h3e n/a
7 TRCN0000242124 GAGATCGCGCAGGACTTCAAG pLKO_005 260 CDS 100% 1.650 0.825 Y Hist1h3d n/a
8 TRCN0000092972 GAAGTCGACCGAGCTGCTGAT pLKO.1 208 CDS 100% 1.350 0.675 Y Hist1h3e n/a
9 TRCN0000248207 GATCGCGCAGGACTTCAAGAC pLKO_005 262 CDS 100% 1.350 0.675 Y Hist1h3b n/a
10 TRCN0000242125 AGAAGTCGACCGAGCTGCTGA pLKO_005 207 CDS 100% 0.880 0.440 Y Hist1h3d n/a
11 TRCN0000092884 CGTCACCATCATGCCCAAGGA pLKO.1 391 CDS 100% 0.880 0.440 Y Hist2h3c2 n/a
12 TRCN0000262271 CAGGACTTCAAGACGGACCTG pLKO_005 269 CDS 100% 0.720 0.360 Y Hist2h3b n/a
13 TRCN0000092971 CTACCAGAAGTCGACCGAGCT pLKO.1 202 CDS 100% 0.720 0.360 Y Hist1h3e n/a
14 TRCN0000092855 GCAGGACTTCAAGACGGACCT pLKO.1 268 CDS 100% 0.720 0.360 Y Hist2h3c1 n/a
15 TRCN0000262273 AGTCGACCGAGCTGCTGATCC pLKO_005 210 CDS 100% 0.000 0.000 Y Hist2h3b n/a
16 TRCN0000248206 ATCCGCAAGCTGCCGTTCCAG pLKO_005 227 CDS 100% 0.000 0.000 Y Hist1h3b n/a
17 TRCN0000092886 CCACGCCAAACGCGTCACCAT pLKO.1 379 CDS 100% 0.000 0.000 Y Hist2h3c2 n/a
18 TRCN0000351852 CCACGCCAAACGCGTCACCAT pLKO_005 379 CDS 100% 0.000 0.000 Y Hist2h3c2 n/a
19 TRCN0000092968 CCAGAAGTCGACCGAGCTGCT pLKO.1 205 CDS 100% 0.000 0.000 Y Hist1h3e n/a
20 TRCN0000092809 CGAGCTGCTGATCCGCAAGCT pLKO.1 217 CDS 100% 0.000 0.000 Y Hist1h3h n/a
21 TRCN0000092854 CGTGAAGAAGCCGCACCGCTA pLKO.1 145 CDS 100% 0.000 0.000 Y Hist2h3c1 n/a
22 TRCN0000092887 CTGTGCGCCATCCACGCCAAA pLKO.1 368 CDS 100% 0.000 0.000 Y Hist2h3c2 n/a
23 TRCN0000092810 CTTCCAGAGCTCGGCCGTCAT pLKO.1 292 CDS 100% 0.000 0.000 Y Hist1h3h n/a
24 TRCN0000282160 GCGAGATCGCGCAGGACTTCA pLKO_005 258 CDS 100% 0.000 0.000 Y Hist2h3b n/a
25 TRCN0000092840 GCGCAAGCAGCTGGCCACCAA pLKO.1 91 CDS 100% 0.000 0.000 Y Hist1h3i n/a
26 TRCN0000092792 GCGCGAGATCGCGCAGGACTT pLKO.1 256 CDS 100% 0.000 0.000 Y Hist1h3a n/a
27 TRCN0000092812 GCGCTACCAGAAGTCGACCGA pLKO.1 199 CDS 100% 0.000 0.000 Y Hist1h3h n/a
28 TRCN0000092811 GCTGATCCGCAAGCTGCCGTT pLKO.1 223 CDS 100% 0.000 0.000 Y Hist1h3h n/a
29 TRCN0000282161 GTGAAGAAGCCGCACCGCTAC pLKO_005 146 CDS 100% 0.000 0.000 Y Hist2h3b n/a
30 TRCN0000257687 TACCAGAAGTCGACCGAGCTG pLKO_005 203 CDS 100% 0.000 0.000 Y Hist1h3b n/a
31 TRCN0000262272 TCCGCAAGCTGCCGTTCCAGC pLKO_005 228 CDS 100% 0.000 0.000 Y Hist2h3b n/a
32 TRCN0000106757 GCGAGATCGCGCAGGACTTTA pLKO.1 258 CDS 100% 4.400 2.200 Y H3C14 n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript NM_178215.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_04820 pDONR223 100% 93.3% 100% None (many diffs) n/a
2 ccsbBroad304_04820 pLX_304 0% 93.3% 100% V5 (many diffs) n/a
3 TRCN0000470519 GCCGATTGAGCTCTTAAAAGGAAC pLX_317 100% 93.3% 100% V5 (many diffs) n/a
4 ccsbBroadEn_07214 pDONR223 100% 88.9% 96.3% None (many diffs) n/a
5 ccsbBroad304_07214 pLX_304 0% 88.9% 96.3% V5 (many diffs) n/a
6 TRCN0000477997 GCACAAGGTCGTGAATTCAGTTAA pLX_317 34.1% 88.9% 96.3% V5 (many diffs) n/a
7 ccsbBroadEn_07225 pDONR223 100% 87.7% 98.5% None (many diffs) n/a
8 ccsbBroad304_07225 pLX_304 0% 87.7% 98.5% V5 (many diffs) n/a
9 ccsbBroadEn_13992 pDONR223 100% 87.5% 7.5% None (many diffs) n/a
10 ccsbBroad304_13992 pLX_304 0% 87.5% 7.5% V5 (not translated due to prior stop codon) (many diffs) n/a
11 TRCN0000474854 TCCGGCCCACTCTACGTTTGCATT pLX_317 100% 87.5% 7.5% V5 (not translated due to prior stop codon) (many diffs) n/a
12 ccsbBroadEn_01906 pDONR223 100% 87.2% 99.2% None (many diffs) n/a
13 ccsbBroad304_01906 pLX_304 0% 87.2% 99.2% V5 (many diffs) n/a
14 TRCN0000469791 ACACCATCTCGTTTATTTCAGCTA pLX_317 77.1% 87.2% 99.2% V5 (many diffs) n/a
15 ccsbBroadEn_01908 pDONR223 100% 87.2% 99.2% None (many diffs) n/a
16 ccsbBroad304_01908 pLX_304 0% 87.2% 99.2% V5 (many diffs) n/a
17 TRCN0000467485 CTCAATTAACCTTCGGAGAAGTTC pLX_317 89.4% 87.2% 99.2% V5 (many diffs) n/a
18 ccsbBroadEn_07226 pDONR223 100% 87% 99.2% None (many diffs) n/a
19 ccsbBroad304_07226 pLX_304 0% 87% 99.2% V5 (many diffs) n/a
20 TRCN0000465999 CATGGCGATGGACTCTTGTAAGTC pLX_317 82.1% 87% 99.2% V5 (many diffs) n/a
21 ccsbBroadEn_01905 pDONR223 100% 86.2% 99.2% None (many diffs) n/a
22 ccsbBroad304_01905 pLX_304 0% 86.2% 99.2% V5 (many diffs) n/a
23 TRCN0000469866 TCGCGAGAACCCACGTCATTTGGC pLX_317 98.6% 86.2% 99.2% V5 (many diffs) n/a
24 ccsbBroadEn_01907 pDONR223 100% 85.5% 99.2% None (many diffs) n/a
25 ccsbBroad304_01907 pLX_304 0% 85.5% 99.2% V5 (many diffs) n/a
26 TRCN0000469614 CTGCCGATCGGACTGCTGGCTACG pLX_317 100% 85.5% 99.2% V5 (many diffs) n/a
27 ccsbBroadEn_07333 pDONR223 100% 85.5% 99.2% None (many diffs) n/a
28 ccsbBroad304_07333 pLX_304 0% 85.5% 99.2% V5 (many diffs) n/a
29 TRCN0000468120 GCAGCACCCCACCATAATCTTTGA pLX_317 96% 85.5% 99.2% V5 (many diffs) n/a
30 ccsbBroadEn_02054 pDONR223 100% 85.2% 99.2% None (many diffs) n/a
31 ccsbBroad304_02054 pLX_304 0% 85.2% 99.2% V5 (many diffs) n/a
32 TRCN0000474049 ACGAGTAGAGTAGCATGTAAGCCT pLX_317 92% 85.2% 99.2% V5 (many diffs) n/a
33 ccsbBroadEn_01904 pDONR223 100% 85.2% 99.2% None (many diffs) n/a
34 ccsbBroad304_01904 pLX_304 97.8% 85.2% 99.2% V5 (many diffs) n/a
35 TRCN0000465966 TGCACCAAATCGTATAGACTTAAT pLX_317 74.7% 85.2% 99.2% V5 (many diffs) n/a
36 ccsbBroadEn_07332 pDONR223 100% 85.2% 99.2% None (many diffs) n/a
37 ccsbBroad304_07332 pLX_304 0% 85.2% 99.2% V5 (many diffs) n/a
38 TRCN0000475169 CTGATGTGCTCCCTCCTTGCCCTC pLX_317 57.4% 85.2% 99.2% V5 (many diffs) n/a
39 ccsbBroadEn_01909 pDONR223 100% 83.4% 99.2% None (many diffs) n/a
40 ccsbBroad304_01909 pLX_304 97.2% 83.4% 99.2% V5 (many diffs) n/a
41 TRCN0000479054 GCGCGGCCAACGTGTTATAATTCG pLX_317 93.7% 83.4% 99.2% V5 (many diffs) n/a
42 ccsbBroadEn_00719 pDONR223 100% 82.8% 97% None (many diffs) n/a
43 ccsbBroad304_00719 pLX_304 0% 82.8% 97% V5 (many diffs) n/a
44 TRCN0000473475 ACAACCTAGAGAAGGGCCGCGTGA pLX_317 92% 82.8% 97% V5 (many diffs) n/a
45 ccsbBroadEn_10159 pDONR223 100% 80.6% 93.3% None (many diffs) n/a
46 ccsbBroad304_10159 pLX_304 0% 80.6% 93.3% V5 (many diffs) n/a
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