Construct: ORF TRCN0000489471

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF020134.1_s317c1
DNA Barcode:
GTGAGACGATACCTATTGCCACGT
Epitope Tag:
V5 (not translated due to prior stop codon)
Notes:
Has stop codon in insert

Originally Annotated References:

Gene:
MAPK8 (5599)

Vector Information:

Vector Backbone:
pLX_317
Pol II Cassette 1:
SV40-PuroR
Pol II Cassette 2:
EF1a-TRCN0000489471
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 5599 MAPK8 mitogen-activated protein k... NM_001323302.2 100% 100%
2 human 5599 MAPK8 mitogen-activated protein k... NM_001323324.2 100% 100%
3 human 5599 MAPK8 mitogen-activated protein k... NM_001323326.2 100% 100%
4 human 5599 MAPK8 mitogen-activated protein k... NM_001323327.2 100% 100%
5 human 5599 MAPK8 mitogen-activated protein k... NM_001323321.2 97.4% 97.9% (many diffs)
6 human 5599 MAPK8 mitogen-activated protein k... NM_001323325.2 97.4% 97.9% (many diffs)
7 human 5599 MAPK8 mitogen-activated protein k... NM_139046.4 97.4% 97.9% (many diffs)
8 human 5599 MAPK8 mitogen-activated protein k... XM_024448080.1 97.4% 97.9% (many diffs)
9 human 5599 MAPK8 mitogen-activated protein k... NM_001323328.2 89.1% 88.7% 1136_1137insAGCAC;1148_1281del
10 human 5599 MAPK8 mitogen-activated protein k... NM_001323329.2 89.1% 88.7% 1136_1137insAGCAC;1148_1281del
11 human 5599 MAPK8 mitogen-activated protein k... NM_001323331.2 89.1% 88.7% 1136_1137insAGCAC;1148_1281del
12 human 5599 MAPK8 mitogen-activated protein k... NM_139049.4 89.1% 88.7% 1136_1137insAGCAC;1148_1281del
13 human 5599 MAPK8 mitogen-activated protein k... NM_001278547.1 86.9% 86.8% (many diffs)
14 human 5599 MAPK8 mitogen-activated protein k... NM_001323322.2 86.9% 86.8% (many diffs)
15 human 5599 MAPK8 mitogen-activated protein k... NM_001323323.2 86.9% 86.8% (many diffs)
16 human 5599 MAPK8 mitogen-activated protein k... NM_001323330.2 86.9% 86.8% (many diffs)
17 human 5599 MAPK8 mitogen-activated protein k... XM_024448079.1 86.9% 86.8% (many diffs)
18 human 5599 MAPK8 mitogen-activated protein k... NM_001278548.1 80.2% 80.2% 615_616ins228
19 human 5602 MAPK10 mitogen-activated protein k... NM_001351625.2 79.7% 92.4% (many diffs)
20 human 5602 MAPK10 mitogen-activated protein k... XM_017008434.2 79.7% 92.4% (many diffs)
21 human 5602 MAPK10 mitogen-activated protein k... XM_017008435.2 79.7% 92.4% (many diffs)
22 human 5602 MAPK10 mitogen-activated protein k... XM_017008436.2 79.7% 92.4% (many diffs)
23 human 5602 MAPK10 mitogen-activated protein k... XM_017008437.2 79.7% 92.4% (many diffs)
24 human 5602 MAPK10 mitogen-activated protein k... NM_001363657.2 77.5% 90.8% (many diffs)
25 human 5602 MAPK10 mitogen-activated protein k... XM_017008441.2 73.8% 86.4% (many diffs)
26 human 5602 MAPK10 mitogen-activated protein k... NM_002753.5 72.6% 84.1% (many diffs)
27 human 5602 MAPK10 mitogen-activated protein k... XM_005263135.4 72.6% 84.1% (many diffs)
28 human 5602 MAPK10 mitogen-activated protein k... XM_017008429.2 72.6% 84.1% (many diffs)
29 human 5602 MAPK10 mitogen-activated protein k... XM_017008430.2 72.6% 84.1% (many diffs)
30 human 5602 MAPK10 mitogen-activated protein k... XM_017008432.2 72.6% 84.1% (many diffs)
31 human 5602 MAPK10 mitogen-activated protein k... XM_024454144.1 72.6% 84.1% (many diffs)
32 human 5602 MAPK10 mitogen-activated protein k... XM_024454145.1 72.6% 84.1% (many diffs)
33 human 5602 MAPK10 mitogen-activated protein k... XM_024454146.1 72.6% 84.1% (many diffs)
34 human 5602 MAPK10 mitogen-activated protein k... XM_024454147.1 72.6% 84.1% (many diffs)
35 human 5599 MAPK8 mitogen-activated protein k... NM_001323320.2 71.4% 70.9% 615_616ins228;908_909insAGCAC;920_1053del
36 human 5602 MAPK10 mitogen-activated protein k... NM_001351624.2 71.3% 82.1% (many diffs)
37 human 5602 MAPK10 mitogen-activated protein k... NM_138980.4 71.3% 82.1% (many diffs)
38 human 5602 MAPK10 mitogen-activated protein k... XM_006714268.3 71.3% 82.1% (many diffs)
39 human 5602 MAPK10 mitogen-activated protein k... XM_011532120.3 71.3% 82.1% (many diffs)
40 human 5602 MAPK10 mitogen-activated protein k... XM_011532121.3 71.3% 82.1% (many diffs)
41 human 5602 MAPK10 mitogen-activated protein k... XM_017008423.2 71.3% 82.1% (many diffs)
42 human 5602 MAPK10 mitogen-activated protein k... XM_006714269.3 70.6% 82.7% (many diffs)
43 human 5602 MAPK10 mitogen-activated protein k... XM_017008433.2 65.9% 76.7% (many diffs)
44 human 5602 MAPK10 mitogen-activated protein k... NM_138982.4 65.5% 75.4% (many diffs)
45 human 5602 MAPK10 mitogen-activated protein k... XM_005263129.3 65.5% 75.4% (many diffs)
46 human 5602 MAPK10 mitogen-activated protein k... XM_005263130.3 65.5% 75.4% (many diffs)
47 human 5602 MAPK10 mitogen-activated protein k... XM_011532117.3 65.5% 75.4% (many diffs)
48 human 5602 MAPK10 mitogen-activated protein k... XM_011532118.3 65.5% 75.4% (many diffs)
49 human 5602 MAPK10 mitogen-activated protein k... XM_017008420.2 65.5% 75.4% (many diffs)
50 human 5602 MAPK10 mitogen-activated protein k... XM_024454139.1 65.5% 75.4% (many diffs)
51 human 5602 MAPK10 mitogen-activated protein k... XM_024454140.1 65.5% 75.4% (many diffs)
52 human 5602 MAPK10 mitogen-activated protein k... XM_024454141.1 65.5% 75.4% (many diffs)
53 human 5602 MAPK10 mitogen-activated protein k... XM_024454142.1 65.5% 75.4% (many diffs)
54 human 5602 MAPK10 mitogen-activated protein k... XM_024454143.1 65.5% 75.4% (many diffs)
55 human 5602 MAPK10 mitogen-activated protein k... XM_017008451.2 64.5% 74.2% (many diffs)
56 human 5602 MAPK10 mitogen-activated protein k... NM_001318069.2 63.6% 75.4% (many diffs)
57 human 5602 MAPK10 mitogen-activated protein k... XM_017008422.2 60.5% 70.4% (many diffs)
58 human 5602 MAPK10 mitogen-activated protein k... XM_017008452.2 58.2% 66.9% (many diffs)
59 human 5602 MAPK10 mitogen-activated protein k... XM_017008445.2 57.6% 65.7% (many diffs)
60 human 5602 MAPK10 mitogen-activated protein k... XM_017008447.2 52% 59.1% (many diffs)
61 human 5602 MAPK10 mitogen-activated protein k... XM_017008448.2 52% 59.1% (many diffs)
62 human 5602 MAPK10 mitogen-activated protein k... XM_017008454.2 39% 46.4% (many diffs)
63 human 5602 MAPK10 mitogen-activated protein k... XM_024454149.1 39% 46.4% (many diffs)
64 human 5602 MAPK10 mitogen-activated protein k... XM_017008453.2 38.9% 46.6% (many diffs)
65 human 5599 MAPK8 mitogen-activated protein k... NR_136583.2 19.7% 1_194del;1063_1064insAGCC;1343_5810del
66 human 5599 MAPK8 mitogen-activated protein k... NR_136584.2 19.6% (many diffs)
67 human 5599 MAPK8 mitogen-activated protein k... NR_136585.2 19.3% (many diffs)
68 mouse 26419 Mapk8 mitogen-activated protein k... NM_016700.4 94% 100% (many diffs)
69 mouse 26419 Mapk8 mitogen-activated protein k... NM_001310452.1 91.6% 97.9% (many diffs)
70 mouse 26419 Mapk8 mitogen-activated protein k... XM_006519032.3 91.6% 97.9% (many diffs)
71 mouse 26419 Mapk8 mitogen-activated protein k... NM_001310454.1 83.9% 88.7% (many diffs)
72 mouse 26419 Mapk8 mitogen-activated protein k... NM_001310453.1 81.7% 86.8% (many diffs)
73 mouse 26414 Mapk10 mitogen-activated protein k... XM_017320890.2 77.8% 92.4% (many diffs)
74 mouse 26414 Mapk10 mitogen-activated protein k... XM_017320891.2 77.8% 92.4% (many diffs)
75 mouse 26414 Mapk10 mitogen-activated protein k... XM_030254519.1 75.9% 91.4% (many diffs)
76 mouse 26414 Mapk10 mitogen-activated protein k... XM_017320888.2 69.6% 82.1% (many diffs)
77 mouse 26414 Mapk10 mitogen-activated protein k... XM_006534950.4 69.1% 83.1% (many diffs)
78 mouse 26414 Mapk10 mitogen-activated protein k... XM_030254516.1 63.9% 75.4% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
72
ORF end:
1224
ORF length:
1152
Sequence:
1tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag
61caggcttcac catgagcaga agcaagcgtg acaacaattt ttatagtgta gagattggag
121attctacatt cacagtcctg aaacgatatc agaatttaaa acctataggc tcaggagctc
181aaggaatagt atgcgcagct tatgatgcca ttcttgaaag aaatgttgca atcaagaagc
241taagccgacc atttcagaat cagactcatg ccaagcgggc ctacagagag ctagttctta
301tgaaatgtgt taatcacaaa aatataattg gccttttgaa tgttttcaca ccacagaaat
361ccctagaaga atttcaagat gtttacatag tcatggagct catggatgca aatctttgcc
421aagtgattca gatggagcta gatcatgaaa gaatgtccta ccttctctat cagatgctgt
481gtggaatcaa gcaccttcat tctgctggaa ttattcatcg ggacttaaag cccagtaata
541tagtagtaaa atctgattgc actttgaaga ttcttgactt cggtctggcc aggactgcag
601gaacgagttt tatgatgacg ccttatgtag tgactcgcta ctacagagca cccgaggtca
661tccttggcat gggctacaag gaaaacgtgg atttatggtc tgtggggtgc attatgggag
721aaatggtttg ccacaaaatc ctctttccag gaagggacta tattgatcag tggaataaag
781ttattgaaca gcttggaaca ccatgtcctg aattcatgaa gaaactgcaa ccaacagtaa
841ggacttacgt tGAAAACAGA CCTAAATATG CTGGATATAG CTTTGAGAAA CTCTTCCCTG
901ATGTCCTTTT CCCAGCTGAC TCAGAACACA ACAAACTTAA AGCCAGTCAG GCAAGGGATT
961TGTTATCCAA AATGCTGGTA ATAGATGCAT CTAAAAGGAT CTCTGTAGAT GAAGCTCTCC
1021AACACCCGTA CATCAATGTC TGGTATGATC CTTCTGAAGC AGAAGCTCCA CCACCAAAGA
1081TCCCTGACAA GCAGTTAGAT GAAAGGGAAC ACACAATAGA AGAGTGGAAA GAATTGATAT
1141ATAAGGAAGT TATGGACTTG GAGGAGAGAA CCAAGAATGG AGTTATACGG GGGCAGCCCT
1201CTCCTTTAGC ACAGGTGCAG CAGTGAGACC CAGCTTTCTT GTACAAAGTG GTTGATATCG
1261GTAAGCCTAT CCCTAACCCT CTCCTCGGTC TCGATTCTAC GTAGTAATGA ACTAGTCCGT
1321AACTTGAAAG TATTTCGATT TCTTGGCTTT ATATATCTTG TGGAAAGGAC GAGTGAGACG
1381ATACCTATTG CCACGTACGC GTTAAGTCga caatcaacct ctggattaca aaatttgtga
1441aagatt

Download FASTA (ORF) (Full)