Construct: ORF TRCN0000470042
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF005912.1_s317c1
- Derived from:
- ccsbBroadEn_00472
- DNA Barcode:
- CCGTCATAAGGGACACTGTAAAAG
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- EBF1 (1879)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000470042
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 1879 | EBF1 | EBF transcription factor 1 | NM_024007.5 | 100% | 100% | |
| 2 | human | 1879 | EBF1 | EBF transcription factor 1 | NM_001290360.3 | 99.8% | 99.8% | 778_780delGCA |
| 3 | human | 1879 | EBF1 | EBF transcription factor 1 | NM_001324107.2 | 98.8% | 98.6% | 754_755insGTACGCCCTCTTATCTGGAA;756_757insT |
| 4 | human | 1879 | EBF1 | EBF transcription factor 1 | NM_001324108.2 | 98.6% | 98.6% | 753_754ins24 |
| 5 | human | 1879 | EBF1 | EBF transcription factor 1 | NM_001324103.2 | 97% | 96.1% | (many diffs) |
| 6 | human | 1879 | EBF1 | EBF transcription factor 1 | XM_017009193.1 | 97% | 96.1% | (many diffs) |
| 7 | human | 1879 | EBF1 | EBF transcription factor 1 | NM_001324101.2 | 96.8% | 96% | (many diffs) |
| 8 | human | 1879 | EBF1 | EBF transcription factor 1 | XM_017009192.1 | 96.8% | 96% | (many diffs) |
| 9 | human | 1879 | EBF1 | EBF transcription factor 1 | XM_017009194.1 | 95.8% | 94.8% | (many diffs) |
| 10 | human | 1879 | EBF1 | EBF transcription factor 1 | NM_001324106.2 | 95.7% | 94.8% | (many diffs) |
| 11 | human | 1879 | EBF1 | EBF transcription factor 1 | XM_017009195.1 | 95.7% | 94.8% | (many diffs) |
| 12 | human | 1879 | EBF1 | EBF transcription factor 1 | NM_182708.3 | 94.7% | 94.7% | 482_483ins69;684_685ins24 |
| 13 | human | 1879 | EBF1 | EBF transcription factor 1 | NM_001364156.1 | 93.4% | 92.5% | 4delT;10_11ins38;17_18ins77 |
| 14 | human | 1879 | EBF1 | EBF transcription factor 1 | XM_017009197.1 | 90.6% | 88.9% | (many diffs) |
| 15 | human | 1879 | EBF1 | EBF transcription factor 1 | NM_001364157.1 | 90.4% | 88.7% | (many diffs) |
| 16 | human | 1879 | EBF1 | EBF transcription factor 1 | XM_017009196.1 | 90.4% | 88.7% | (many diffs) |
| 17 | human | 1879 | EBF1 | EBF transcription factor 1 | NM_001364155.1 | 89.8% | 89.6% | 1369_1370ins180 |
| 18 | human | 1879 | EBF1 | EBF transcription factor 1 | NM_001324109.2 | 86.9% | 85.9% | (many diffs) |
| 19 | human | 1879 | EBF1 | EBF transcription factor 1 | NM_001364159.1 | 77.4% | 77.4% | 0_1ins399 |
| 20 | human | 1879 | EBF1 | EBF transcription factor 1 | NM_001324111.2 | 77.3% | 77.3% | 0_1ins399;379_381delGCA |
| 21 | human | 1879 | EBF1 | EBF transcription factor 1 | XM_024454393.1 | 77.3% | 77.3% | 0_1ins399;379_381delGCA |
| 22 | human | 1879 | EBF1 | EBF transcription factor 1 | NM_001364158.1 | 76.1% | 76.1% | 0_1ins399;354_355ins24 |
| 23 | human | 1879 | EBF1 | EBF transcription factor 1 | XM_024454390.1 | 74.9% | 74% | (many diffs) |
| 24 | human | 1879 | EBF1 | EBF transcription factor 1 | XM_024454391.1 | 74.9% | 74% | (many diffs) |
| 25 | human | 1879 | EBF1 | EBF transcription factor 1 | XM_024454392.1 | 73.7% | 72.8% | (many diffs) |
| 26 | human | 1879 | EBF1 | EBF transcription factor 1 | XM_017009201.2 | 64.9% | 64.9% | 0_1ins621 |
| 27 | human | 1879 | EBF1 | EBF transcription factor 1 | XM_017009199.1 | 62.7% | 61.8% | (many diffs) |
| 28 | human | 1879 | EBF1 | EBF transcription factor 1 | XM_017009200.2 | 62.7% | 61.8% | (many diffs) |
| 29 | human | 1879 | EBF1 | EBF transcription factor 1 | XM_017009203.1 | 60.1% | 59.2% | (many diffs) |
| 30 | human | 1879 | EBF1 | EBF transcription factor 1 | XM_017009202.1 | 60% | 59.1% | (many diffs) |
| 31 | human | 1879 | EBF1 | EBF transcription factor 1 | XM_017009204.1 | 52.3% | 47.1% | (many diffs) |
| 32 | mouse | 13591 | Ebf1 | early B cell factor 1 | NM_007897.3 | 94% | 100% | (many diffs) |
| 33 | mouse | 13591 | Ebf1 | early B cell factor 1 | NM_001290709.1 | 93.9% | 99.8% | (many diffs) |
| 34 | mouse | 13591 | Ebf1 | early B cell factor 1 | XM_006532155.3 | 92.8% | 98.6% | (many diffs) |
| 35 | mouse | 13591 | Ebf1 | early B cell factor 1 | XM_006532156.3 | 92.7% | 98.6% | (many diffs) |
| 36 | mouse | 13591 | Ebf1 | early B cell factor 1 | XM_006532157.3 | 87.4% | 92.9% | (many diffs) |
| 37 | mouse | 13591 | Ebf1 | early B cell factor 1 | NM_001290710.1 | 83.4% | 88.3% | (many diffs) |
| 38 | mouse | 13591 | Ebf1 | early B cell factor 1 | NM_001290711.1 | 72.5% | 77.4% | (many diffs) |
| 39 | mouse | 13591 | Ebf1 | early B cell factor 1 | XM_006532158.3 | 72.4% | 77.3% | (many diffs) |
| 40 | mouse | 13591 | Ebf1 | early B cell factor 1 | XM_017314250.1 | 72.4% | 77.3% | (many diffs) |
| 41 | mouse | 13591 | Ebf1 | early B cell factor 1 | XM_017314251.1 | 71.2% | 76.1% | (many diffs) |
| 42 | mouse | 13591 | Ebf1 | early B cell factor 1 | XM_017314252.1 | 71.2% | 76.1% | (many diffs) |
| 43 | mouse | 13593 | Ebf3 | early B cell factor 3 | XM_011241672.1 | 70.9% | 80.9% | (many diffs) |
| 44 | mouse | 13593 | Ebf3 | early B cell factor 3 | XM_006507334.1 | 68.2% | 76.3% | (many diffs) |
| 45 | mouse | 13593 | Ebf3 | early B cell factor 3 | XM_006507337.3 | 63.7% | 72.3% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1839
- ORF length:
- 1773
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgtt tgggattcag gaaagcatcc aacggagtgg aagcagcatg aaggaagagc 121 cgctgggcag cggcatgaac gcggtgcgga cgtggatgca gggcgccggg gtgctggacg 181 ccaacacggc ggcgcagagc ggggtgggtc tggcccgggc tcactttgag aagcagccgc 241 cttccaatct gcggaaatcc aacttcttcc acttcgtcct ggccctctac gacagacagg 301 gccagcccgt ggagatcgag aggacagcgt ttgtggggtt cgtggagaag gaaaaagaag 361 ccaacagcga aaagaccaat aacggaattc actaccggct tcagcttctc tacagcaatg 421 ggataaggac ggagcaggat ttctacgtgc gcctcattga ctccatgaca aaacaagcca 481 tagtgtatga aggccaagac aagaacccag aaatgtgccg agtcttgctc acacatgaga 541 tcatgtgcag ccgctgttgt gacaagaaaa gctgtggcaa ccgaaatgag actccctcag 601 atccagtgat aattgacagg ttcttcttga aatttttcct caaatgtaac caaaattgcc 661 taaagaatgc gggaaaccca cgtgacatgc ggagattcca ggtcgtggtg tctacgacag 721 tcaatgtgga tggccatgtc ctggcagtct ctgataacat gtttgtccat aataattcca 781 agcatgggcg gagggctcgg aggcttgacc cctcggaagg tacgccctct tatctggaac 841 atgctactcc ctgtatcaaa gccatcagcc cgagtgaagg atggacgacg ggaggtgcga 901 ctgtgatcat cataggggac aatttctttg atgggttaca ggtcatattc ggtaccatgc 961 tggtctggag tgagttgatc actcctcatg ccatccgtgt gcagacccct cctcggcaca 1021 tccctggtgt tgtggaagtc acactgtcct acaaatctaa gcagttctgc aaaggaacac 1081 caggcagatt catttataca gcgctcaacg aacccaccat cgattatggt ttccagaggt 1141 tacagaaggt cattcctcgg caccctggtg accctgagcg tttgccaaag gaagtaatac 1201 tcaaaagggc tgcggatctg gtagaagcac tgtatgggat gccacacaac aaccaggaaa 1261 tcattctgaa gagagcggcc gacattgccg aggccctgta cagtgttccc cgcaaccaca 1321 accaactccc ggcccttgct aacacctcgg tccacgcagg gatgatgggc gtgaattcgt 1381 TCAGTGGACA ACTGGCCGTG AATGTCTCCG AGGCATCACA AGCCACCAAT CAGGGTTTCA 1441 CCCGCAACTC AAGCAGCGTA TCACCACACG GGTACGTGCC GAGCACCACT CCCCAGCAGA 1501 CCAACTATAA CTCCGTCACC ACGAGCATGA ACGGATACGG CTCTGCCGCA ATGTCCAATT 1561 TGGGCGGCTC CCCCACCTTC CTCAACGGCT CAGCTGCCAA CTCCCCCTAT GCCATAGTGC 1621 CATCCAGCCC CACCATGGCC TCCTCCACAA GCCTCCCCTC CAACTGCAGC AGCTCCTCGG 1681 GCATCTTCTC CTTCTCACCA GCCAACATGG TCTCAGCCGT GAAACAGAAG AGTGCTTTCG 1741 CACCAGTCGT CAGACCCCAG ACCTCCCCAC CTCCCACCTG CACCAGCACC AACGGGAACA 1801 GCCTGCAAGC GATATCTGGC ATGATTGTTC CTCCTATGTG CCCAACTTTC TTGTACAAAG 1861 TGGTTGATAT CGGTAAGCCT ATCCCTAACC CTCTCCTCGG TCTCGATTCT ACGTAGTAAT 1921 GAACTAGTCC GTAACTTGAA AGTATTTCGA TTTCTTGGCT TTATATATCT TGTGGAAAGG 1981 ACGACCGTCA TAAGGGACAC TGTAAAAGAC GCGTTAAGTC gacaatcaac ctctggatta 2041 caaaatttgt gaaagatt