Construct: ORF TRCN0000491572

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF017983.1_s317c1
Derived from:
ccsbBroadEn_12975
DNA Barcode:
TCTTGCAAAAGGGGAGCATACACC
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
INTS4 (92105)

Vector Information:

Vector Backbone:
pLX_317
Pol II Cassette 1:
SV40-PuroR
Pol II Cassette 2:
EF1a-TRCN0000491572
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 92105 INTS4 integrator complex subunit 4 XR_001748041.2 69.2% (many diffs)
2 human 644619 INTS4P2 integrator complex subunit ... NR_027392.2 64.9% (many diffs)
3 human 92105 INTS4 integrator complex subunit 4 XR_002957209.1 59.8% 1_361del;389_495del;1999_2555del
4 human 92105 INTS4 integrator complex subunit 4 XM_011545352.2 59% 56.5% (many diffs)
5 human 92105 INTS4 integrator complex subunit 4 XM_017018560.2 55.3% 53% (many diffs)
6 human 92105 INTS4 integrator complex subunit 4 XM_011545353.3 54.9% 53.3% (many diffs)
7 human 92105 INTS4 integrator complex subunit 4 NM_033547.4 52.3% 50.1% (many diffs)
8 human 92105 INTS4 integrator complex subunit 4 XR_001748037.2 49.4% (many diffs)
9 human 92105 INTS4 integrator complex subunit 4 XR_001748036.2 48.5% (many diffs)
10 human 92105 INTS4 integrator complex subunit 4 XR_001748039.2 46.3% (many diffs)
11 human 92105 INTS4 integrator complex subunit 4 XR_001748040.2 45.1% (many diffs)
12 human 285905 INTS4P1 integrator complex subunit ... NR_146905.1 39.5% (many diffs)
13 human 285905 INTS4P1 integrator complex subunit ... NR_146906.1 35.4% (many diffs)
14 human 92105 INTS4 integrator complex subunit 4 XR_002957208.1 28.7% (many diffs)
15 mouse 101861 Ints4 integrator complex subunit 4 XM_006507179.3 47.9% 48.9% (many diffs)
16 mouse 101861 Ints4 integrator complex subunit 4 NM_027256.2 47.9% 48.8% (many diffs)
17 mouse 101861 Ints4 integrator complex subunit 4 XM_006507180.1 47% 47.9% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
66
ORF end:
1596
ORF length:
1530
Sequence:
1tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag
61ttggcatgcc atcaacatcc tgcagaatga aacatctgac agatacgtct catggtgtaa
121gaaataagtg cctgcagtta cttggcaatc ttggctcttt ggagaaaagt gtcacaaaag
181atgcagaagg cctagctgcc agagatgtcc agaagattat aggggattac ttcagtgacc
241aagacccacg tgtcagaaca gcagctataa aagccatgtt gcagctccat gaaagaggac
301tgaaattaca ccaaacaatt tataatcagg cctgtaaatt actctctgat gactatgaac
361aagtgcgcag tgctgcagtc cagcttatct gggtcgtcag tcagctctat cctgaaagca
421ttgtcccaat tccttcttct aatgaagaaa tacgcttagt tgatgatgcg tttggcaaaa
481tttgtcacat ggtcagtgat ggctcttggg tggttcgtgt tcaggcagca aaactgttgg
541gctctatgga gcaagtcagt tctcatttct tggagcagac ccttgacaag aagctgatgt
601cagatctgag gaggaaacgt actgcacatg agcgtgccaa ggaactttac agttcggggg
661agttttccag tggcagaaag tggggagatg atgctcccaa ggaagaagta gataccgggg
721ctgtgaactt gattgagtca ggagcttgtg gagcttttgt tcatgggttg gaagatgaga
781tgtatgaggt tcgtattgct gctgtggagg ccctctgcat gttggcccag tcttcaccct
841cttttgctga gaagtgcctt gatttcctag ttgacatgtt caacgatgaa attgaggaag
901tacgtctgca gtctatacat accatgagaa aaatctctaa caacatcacc ctccgagaag
961atcagcttga cactgtcctg gctgtgctag aggattcatc cagagatatt cgagaggctc
1021ttcatgaact cttatgctgt actaatgttt caaccaaaga agggattcat cttgcattgg
1081tggagctgct gaaaaattta accaagtacc ctactgatag ggactccata tggaagtgct
1141tgaagtttct gggaagtcgg catccaaccc tggtgcttcc cttggtgcca gagcttctga
1201gcacccaccc attttttGAC ACAGCTGAAC CAGACATGGA TGATCCAGCT TATATTGCAG
1261TTTTGGTACT TATTTTCAAT GCTGCTAAAA CCTGTCCAAC AATGCCAGCA TTGTTCTCAG
1321ATCACACCTT CAGGCACTAT GCCTACCTCC GAGACAGTCT TTCTCATCTT GTTCCTGCCT
1381TGAGGTTACC AGGTAGAAAA CTGGTGTCAT CAGCTGTTTC TCCCAGCATC ATACCTCAAG
1441AGGATCCTTC CCAGCAGTTC CTGCAGCAGA GCCTTGAAAG AGTGTATAGT CTTCAGCACT
1501TGGACCCTCA GGGAGCCCAG GAGCTGCTGG AATTCACCAT CAGAGTCGAG GTTTCACCAT
1561GGTGGCCAGG CTGGTCTCAA ACTCCTGAGC TCAAGTGCCC AACTTTCTTG TACAAAGTGG
1621TTGATATCGG TAAGCCTATC CCTAACCCTC TCCTCGGTCT CGATTCTACG TAGTAATGAA
1681CTAGTCCGTA ACTTGAAAGT ATTTCGATTT CTTGGCTTTA TATATCTTGT GGAAAGGACG
1741ATCTTGCAAA AGGGGAGCAT ACACCACGCG TTAAGTCgac aatcaacctc tggattacaa
1801aatttgtgaa agatt

Download FASTA (ORF) (Full)