Construct: ORF TRCN0000489338

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF019695.1_s317c1
DNA Barcode:
CTCATGACGTTAAATTTCAGTTAA
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
MAPK8 (5599)

Vector Information:

Vector Backbone:
pLX_317
Pol II Cassette 1:
SV40-PuroR
Pol II Cassette 2:
EF1a-TRCN0000489338
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 5599 MAPK8 mitogen-activated protein k... NM_001323302.2 99.9% 99.7% 1152_1153insG
2 human 5599 MAPK8 mitogen-activated protein k... NM_001323324.2 99.9% 99.7% 1152_1153insG
3 human 5599 MAPK8 mitogen-activated protein k... NM_001323326.2 99.9% 99.7% 1152_1153insG
4 human 5599 MAPK8 mitogen-activated protein k... NM_001323327.2 99.9% 99.7% 1152_1153insG
5 human 5599 MAPK8 mitogen-activated protein k... NM_001323321.2 97.3% 97.6% (many diffs)
6 human 5599 MAPK8 mitogen-activated protein k... NM_001323325.2 97.3% 97.6% (many diffs)
7 human 5599 MAPK8 mitogen-activated protein k... NM_139046.4 97.3% 97.6% (many diffs)
8 human 5599 MAPK8 mitogen-activated protein k... XM_024448080.1 97.3% 97.6% (many diffs)
9 human 5599 MAPK8 mitogen-activated protein k... NM_001323328.2 89.1% 88.7% 1136_1137insAGCAC;1148_1281delinsG
10 human 5599 MAPK8 mitogen-activated protein k... NM_001323329.2 89.1% 88.7% 1136_1137insAGCAC;1148_1281delinsG
11 human 5599 MAPK8 mitogen-activated protein k... NM_001323331.2 89.1% 88.7% 1136_1137insAGCAC;1148_1281delinsG
12 human 5599 MAPK8 mitogen-activated protein k... NM_139049.4 89.1% 88.7% 1136_1137insAGCAC;1148_1281delinsG
13 human 5599 MAPK8 mitogen-activated protein k... NM_001278547.1 86.9% 86.8% (many diffs)
14 human 5599 MAPK8 mitogen-activated protein k... NM_001323322.2 86.9% 86.8% (many diffs)
15 human 5599 MAPK8 mitogen-activated protein k... NM_001323323.2 86.9% 86.8% (many diffs)
16 human 5599 MAPK8 mitogen-activated protein k... NM_001323330.2 86.9% 86.8% (many diffs)
17 human 5599 MAPK8 mitogen-activated protein k... XM_024448079.1 86.9% 86.8% (many diffs)
18 human 5599 MAPK8 mitogen-activated protein k... NM_001278548.1 80.1% 80% 615_616ins228;924_925insG
19 human 5602 MAPK10 mitogen-activated protein k... NM_001351625.2 79.7% 92.2% (many diffs)
20 human 5602 MAPK10 mitogen-activated protein k... XM_017008434.2 79.7% 92.2% (many diffs)
21 human 5602 MAPK10 mitogen-activated protein k... XM_017008435.2 79.7% 92.2% (many diffs)
22 human 5602 MAPK10 mitogen-activated protein k... XM_017008436.2 79.7% 92.2% (many diffs)
23 human 5602 MAPK10 mitogen-activated protein k... XM_017008437.2 79.7% 92.2% (many diffs)
24 human 5602 MAPK10 mitogen-activated protein k... NM_001363657.2 77.5% 90.6% (many diffs)
25 human 5602 MAPK10 mitogen-activated protein k... XM_017008441.2 73.7% 86.2% (many diffs)
26 human 5602 MAPK10 mitogen-activated protein k... NM_002753.5 72.5% 83.9% (many diffs)
27 human 5602 MAPK10 mitogen-activated protein k... XM_005263135.4 72.5% 83.9% (many diffs)
28 human 5602 MAPK10 mitogen-activated protein k... XM_017008429.2 72.5% 83.9% (many diffs)
29 human 5602 MAPK10 mitogen-activated protein k... XM_017008430.2 72.5% 83.9% (many diffs)
30 human 5602 MAPK10 mitogen-activated protein k... XM_017008432.2 72.5% 83.9% (many diffs)
31 human 5602 MAPK10 mitogen-activated protein k... XM_024454144.1 72.5% 83.9% (many diffs)
32 human 5602 MAPK10 mitogen-activated protein k... XM_024454145.1 72.5% 83.9% (many diffs)
33 human 5602 MAPK10 mitogen-activated protein k... XM_024454146.1 72.5% 83.9% (many diffs)
34 human 5602 MAPK10 mitogen-activated protein k... XM_024454147.1 72.5% 83.9% (many diffs)
35 human 5599 MAPK8 mitogen-activated protein k... NM_001323320.2 71.4% 70.9% 615_616ins228;908_909insAGCAC;920_1053delinsG
36 human 5602 MAPK10 mitogen-activated protein k... NM_001351624.2 71.3% 82.1% (many diffs)
37 human 5602 MAPK10 mitogen-activated protein k... NM_138980.4 71.3% 82.1% (many diffs)
38 human 5602 MAPK10 mitogen-activated protein k... XM_006714268.3 71.3% 82.1% (many diffs)
39 human 5602 MAPK10 mitogen-activated protein k... XM_011532120.3 71.3% 82.1% (many diffs)
40 human 5602 MAPK10 mitogen-activated protein k... XM_011532121.3 71.3% 82.1% (many diffs)
41 human 5602 MAPK10 mitogen-activated protein k... XM_017008423.2 71.3% 82.1% (many diffs)
42 human 5602 MAPK10 mitogen-activated protein k... XM_006714269.3 70.5% 82.5% (many diffs)
43 human 5602 MAPK10 mitogen-activated protein k... XM_017008433.2 65.9% 76.7% (many diffs)
44 human 5602 MAPK10 mitogen-activated protein k... NM_138982.4 65.5% 75.4% (many diffs)
45 human 5602 MAPK10 mitogen-activated protein k... XM_005263129.3 65.5% 75.4% (many diffs)
46 human 5602 MAPK10 mitogen-activated protein k... XM_005263130.3 65.5% 75.4% (many diffs)
47 human 5602 MAPK10 mitogen-activated protein k... XM_011532117.3 65.5% 75.4% (many diffs)
48 human 5602 MAPK10 mitogen-activated protein k... XM_011532118.3 65.5% 75.4% (many diffs)
49 human 5602 MAPK10 mitogen-activated protein k... XM_017008420.2 65.5% 75.4% (many diffs)
50 human 5602 MAPK10 mitogen-activated protein k... XM_024454139.1 65.5% 75.4% (many diffs)
51 human 5602 MAPK10 mitogen-activated protein k... XM_024454140.1 65.5% 75.4% (many diffs)
52 human 5602 MAPK10 mitogen-activated protein k... XM_024454141.1 65.5% 75.4% (many diffs)
53 human 5602 MAPK10 mitogen-activated protein k... XM_024454142.1 65.5% 75.4% (many diffs)
54 human 5602 MAPK10 mitogen-activated protein k... XM_024454143.1 65.5% 75.4% (many diffs)
55 human 5602 MAPK10 mitogen-activated protein k... XM_017008451.2 64.4% 74% (many diffs)
56 human 5602 MAPK10 mitogen-activated protein k... NM_001318069.2 63.6% 75.4% (many diffs)
57 human 5602 MAPK10 mitogen-activated protein k... XM_017008452.2 58.2% 66.7% (many diffs)
58 human 5602 MAPK10 mitogen-activated protein k... XM_017008445.2 57.6% 65.7% (many diffs)
59 human 5602 MAPK10 mitogen-activated protein k... XM_017008447.2 52% 59.1% (many diffs)
60 human 5602 MAPK10 mitogen-activated protein k... XM_017008448.2 52% 59.1% (many diffs)
61 human 5602 MAPK10 mitogen-activated protein k... XM_017008454.2 39% 46.3% (many diffs)
62 human 5602 MAPK10 mitogen-activated protein k... XM_024454149.1 39% 46.3% (many diffs)
63 human 5602 MAPK10 mitogen-activated protein k... XM_017008453.2 38.9% 46.5% (many diffs)
64 human 5599 MAPK8 mitogen-activated protein k... NR_136583.2 19.7% 1_194del;1063_1064insAGCC;1343_5810delinsG
65 human 5599 MAPK8 mitogen-activated protein k... NR_136584.2 19.6% (many diffs)
66 human 5599 MAPK8 mitogen-activated protein k... NR_136585.2 19.3% (many diffs)
67 mouse 26419 Mapk8 mitogen-activated protein k... NM_016700.4 94% 99.7% (many diffs)
68 mouse 26419 Mapk8 mitogen-activated protein k... NM_001310452.1 91.5% 97.6% (many diffs)
69 mouse 26419 Mapk8 mitogen-activated protein k... XM_006519032.3 91.5% 97.6% (many diffs)
70 mouse 26419 Mapk8 mitogen-activated protein k... NM_001310454.1 84% 88.7% (many diffs)
71 mouse 26419 Mapk8 mitogen-activated protein k... NM_001310453.1 81.8% 86.8% (many diffs)
72 mouse 26414 Mapk10 mitogen-activated protein k... XM_017320890.2 77.7% 92.2% (many diffs)
73 mouse 26414 Mapk10 mitogen-activated protein k... XM_017320891.2 77.7% 92.2% (many diffs)
74 mouse 26414 Mapk10 mitogen-activated protein k... XM_030254519.1 75.8% 91.1% (many diffs)
75 mouse 26414 Mapk10 mitogen-activated protein k... XM_017320888.2 69.6% 82.1% (many diffs)
76 mouse 26414 Mapk10 mitogen-activated protein k... XM_006534950.4 69% 82.9% (many diffs)
77 mouse 26414 Mapk10 mitogen-activated protein k... XM_030254516.1 63.9% 75.4% (many diffs)
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Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
75
ORF end:
1230
ORF length:
1155
Sequence:
1tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag
61caggcttcgc caccatgagc agaagcaagc gtgacaacaa tttttatagt gtagagattg
121gagattctac attcacagtc ctgaaacgat atcagaattt aaaacctata ggctcaggag
181ctcaaggaat agtatgcgca gcttatgatg ccattcttga aagaaatgtt gcaatcaaga
241agctaagccg accatttcag aatcagactc atgccaagcg ggcctacaga gagctagttc
301ttatgaaatg tgttaatcac aaaaatataa ttggcctttt gaatgttttc acaccacaga
361aatccctaga agaatttcaa gatgtttaca tagtcatgga gctcatggat gcaaatcttt
421gccaagtgat tcagatggag ctagatcatg aaagaatgtc ctaccttctc tatcagatgc
481tgtgtggaat caagcacctt cattctgctg gaattattca tcgggactta aagcccagta
541atatagtagt aaaatctgat tgcactttga agattcttga cttcggtctg gccaggactg
601caggaacgag ttttatgatg acgccttatg tagtgactcg ctactacaga gcacccgagg
661tcatccttgg catgggctac aaggaaaacg tggatttatg gtctgtgggg tgcattatgg
721gagaaatggt ttgccacaaa atcctctttc caggaaggga ctatattgat cagtggaata
781aagttattga acagcttgga acaccatgtc ctgaattcat gaagaaactg caaccaacag
841taaggactta cgttgaaaac agacctaaat atgctggata tagctttgag aaactcttcc
901cTGATGTCCT TTTCCCAGCT GACTCAGAAC ACAACAAACT TAAAGCCAGT CAGGCAAGGG
961ATTTGTTATC CAAAATGCTG GTAATAGATG CATCTAAAAG GATCTCTGTA GATGAAGCTC
1021TCCAACACCC GTACATCAAT GTCTGGTATG ATCCTTCTGA AGCAGAAGCT CCACCACCAA
1081AGATCCCTGA CAAGCAGTTA GATGAAAGGG AACACACAAT AGAAGAGTGG AAAGAATTGA
1141TATATAAGGA AGTTATGGAC TTGGAGGAGA GAACCAAGAA TGGAGTTATA CGGGGGCAGC
1201CCTCTCCTTT AGCACAGGTG CAGCAGGACC CAGCTTTCTT GTACAAAGTG GTTGATATCG
1261GTAAGCCTAT CCCTAACCCT CTCCTCGGTC TCGATTCTAC GTAGTAATGA ACTAGTCCGT
1321AACTTGAAAG TATTTCGATT TCTTGGCTTT ATATATCTTG TGGAAAGGAC GACTCATGAC
1381GTTAAATTTC AGTTAAACGC GTTAAGTCga caatcaacct ctggattaca aaatttgtga
1441aagatt

Download FASTA (ORF) (Full)