Construct: ORF TRCN0000474479

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF018966.3_s317c1
Derived from:
ccsbBroadEn_14905
DNA Barcode:
AATATATGGTTTCTGATGTGGAAT
Epitope Tag:
V5 (not translated due to prior stop codon)
Notes:
Early stop codon detected

Originally Annotated References:

Gene:
MKNK1 (8569)

Vector Information:

Vector Backbone:
pLX_317
Pol II Cassette 1:
SV40-PuroR
Pol II Cassette 2:
EF1a-TRCN0000474479
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 8569 MKNK1 MAPK interacting serine/thr... NM_003684.7 96.5% 41.7% (many diffs)
2 human 8569 MKNK1 MAPK interacting serine/thr... XM_006711000.1 90.3% 40.4% (many diffs)
3 human 8569 MKNK1 MAPK interacting serine/thr... XM_006711001.3 88.8% 37.8% (many diffs)
4 human 8569 MKNK1 MAPK interacting serine/thr... XM_006711002.3 88.8% 37.8% (many diffs)
5 human 8569 MKNK1 MAPK interacting serine/thr... XM_006711003.1 88.8% 37.8% (many diffs)
6 human 8569 MKNK1 MAPK interacting serine/thr... NM_001135553.4 87.8% 37.6% (many diffs)
7 human 8569 MKNK1 MAPK interacting serine/thr... XM_024450508.1 87.8% 37.6% (many diffs)
8 human 8569 MKNK1 MAPK interacting serine/thr... XM_024450509.1 87.8% 37.6% (many diffs)
9 human 8569 MKNK1 MAPK interacting serine/thr... XM_024450510.1 87.8% 37.6% (many diffs)
10 human 8569 MKNK1 MAPK interacting serine/thr... XM_006711007.1 78.6% 23.9% (many diffs)
11 human 8569 MKNK1 MAPK interacting serine/thr... XM_024450511.1 78.6% 23.9% (many diffs)
12 human 8569 MKNK1 MAPK interacting serine/thr... XM_017002652.2 77% 24.8% (many diffs)
13 human 8569 MKNK1 MAPK interacting serine/thr... XM_017002653.1 77% 24.8% (many diffs)
14 human 8569 MKNK1 MAPK interacting serine/thr... XM_017002659.1 73.8% 49% (many diffs)
15 human 8569 MKNK1 MAPK interacting serine/thr... XM_017002660.2 72.4% 45.9% (many diffs)
16 human 8569 MKNK1 MAPK interacting serine/thr... XM_017002661.2 72.4% 45.9% (many diffs)
17 human 8569 MKNK1 MAPK interacting serine/thr... XM_017002662.1 72.4% 45.9% (many diffs)
18 human 8569 MKNK1 MAPK interacting serine/thr... NM_198973.5 71.3% 45.7% (many diffs)
19 human 8569 MKNK1 MAPK interacting serine/thr... XM_024450512.1 71.3% 45.7% (many diffs)
20 human 8569 MKNK1 MAPK interacting serine/thr... XM_024450513.1 71.3% 45.7% (many diffs)
21 human 8569 MKNK1 MAPK interacting serine/thr... XM_024450514.1 71.3% 45.7% (many diffs)
22 human 8569 MKNK1 MAPK interacting serine/thr... XM_006711013.1 70% 13% (many diffs)
23 human 8569 MKNK1 MAPK interacting serine/thr... XM_006711014.1 70% 13% (many diffs)
24 human 8569 MKNK1 MAPK interacting serine/thr... XM_006711015.1 70% 13% (many diffs)
25 human 8569 MKNK1 MAPK interacting serine/thr... NR_146512.2 43.9% (many diffs)
26 human 8569 MKNK1 MAPK interacting serine/thr... NR_024176.3 41.2% (many diffs)
27 human 8569 MKNK1 MAPK interacting serine/thr... NR_024174.3 41.1% (many diffs)
28 mouse 17346 Mknk1 MAP kinase-interacting seri... NM_001285487.1 77.4% 34.9% (many diffs)
29 mouse 17346 Mknk1 MAP kinase-interacting seri... XM_006502822.3 77.4% 34.9% (many diffs)
30 mouse 17346 Mknk1 MAP kinase-interacting seri... XM_006502823.3 77.1% 35.1% (many diffs)
31 mouse 17346 Mknk1 MAP kinase-interacting seri... NM_001285488.1 76.4% 34.6% (many diffs)
32 mouse 17346 Mknk1 MAP kinase-interacting seri... NM_021461.5 76.4% 34.6% (many diffs)
33 mouse 17346 Mknk1 MAP kinase-interacting seri... XM_006502825.3 64.6% 42.6% (many diffs)
34 mouse 17346 Mknk1 MAP kinase-interacting seri... XM_017320015.1 63.5% 42.3% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
66
ORF end:
696
ORF length:
630
Sequence:
1tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag
61ttggcatggt atcttctcaa aagttggaaa aacctataga gatgggcagt agcgaacccc
121ttcccatcgc agatggtgac aggaggagga agaagaagcg gaggggccgg gccactgact
181ccttgccagg aaagtttgaa gatatgtaca agctgacctc tgaattgctt ggagagggag
241cctatgccaa agttcaaggt gccgtgagcc tacagaatgg caaagagtat gccgtcaaaa
301tcatcgagaa acaagcaggg cacagtcgga gtagggtgtt tcgagaggtg gagacgctgt
361atcagtgtca gggaaacaag aacattttgg agctgattga gttctttgaa gatgacacaa
421ggttttactt ggtctttgag aaattgcaag gaggttccat cttagcccac atccagaagc
481aaaagcactt caatgagcga gaagccagcc gagtggtgcg ggacgttgct gctgcccttg
541acttcctgca taccaaagac aaagtctctc tctgtcacct aggctggagt gctatggcgc
601catcagggct cactgcagcc ccaacctccc tgggctccag tgatcctccc acctcagcct
661cccaagtagc tgggactaca ngcatttgct catngtgatn tgaaaccaag naaaatatat
721tgtgtgaatc tccaggaaaa ggtgtctcca gtgaaaaatc tgtgactttg acttgggcag
781tgggatgaaa ctgaacaact ccttgtaccc ccataaccac accagagctg accaccccat
841gtggctctgc agaatacatg gcccctgaag gtagtngagg tcttcacgga ccaggccaca
901ttctacgaca agcgctgtga cctgtggagc ctgggcgtgg tcctctacat catgctgagt
961ggctacccac ccttcgtggg tcactgcggg gccgactgtg gctgggaccg gggcgaggtc
1021tgcagggtgt gccagaacaa gctgtttgaa agcatccagg aaggCAAGTA TGAGTTTCCT
1081GACAAGGACT GGGCACACAT CTCCAGTGAA GCCAAAGACC TCATCTCCAA GCTCCTGGTG
1141CGAGATGCAA AGCAGAGACT TAGCGCCGCC CAAGTTCTGC AGCACCCATG GGTGCAGGGG
1201CAAGCTCCAG AAAAGGGACT CCCCACGCCG CAAGTCCTCC AGAGGAACAG CAGCACAATG
1261GACCTGACGC TCTTCGCAGC TGAGGCCATC GCCCTTAACC GCCAGCTATC TCAGCACGAA
1321GAGAACGAAC TAGCAGAGGA GCCAGAGGCA CTAGCTGATG GCCTCTGCTC CATGAAGCTT
1381TCCCCTCCCT GCAAGTCACG CCTGGCCCGG AGACGGGCCC TGGCCCAGGC AGGCCGTGGT
1441GAAGACAGGA GCCCGCCCAC AGCACTCTGC CCAACTTTCT TGTACAAAGT GGTTGATATC
1501GGTAAGCCTA TCCCTAACCC TCTCCTCGGT CTCGATTCTA CGTAGTAATG AACTAGTCCG
1561TAACTTGAAA GTATTTCGAT TTCTTGGCTT TATATATCTT GTGGAAAGGA CGAAATATAT
1621GGTTTCTGAT GTGGAATACG CGTTAAGTCg acaatcaacc tctggattac aaaatttgtg
1681aaagatt

Download FASTA (ORF) (Full)