Construct: ORF TRCN0000474479
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF018966.3_s317c1
- Derived from:
- ccsbBroadEn_14905
- DNA Barcode:
- AATATATGGTTTCTGATGTGGAAT
- Epitope Tag:
- V5 (not translated due to prior stop codon)
- Notes:
- Early stop codon detected
Originally Annotated References:
- Gene:
- MKNK1 (8569)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000474479
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 8569 | MKNK1 | MAPK interacting serine/thr... | NM_003684.7 | 96.5% | 41.7% | (many diffs) |
| 2 | human | 8569 | MKNK1 | MAPK interacting serine/thr... | XM_006711000.1 | 90.3% | 40.4% | (many diffs) |
| 3 | human | 8569 | MKNK1 | MAPK interacting serine/thr... | XM_006711001.3 | 88.8% | 37.8% | (many diffs) |
| 4 | human | 8569 | MKNK1 | MAPK interacting serine/thr... | XM_006711002.3 | 88.8% | 37.8% | (many diffs) |
| 5 | human | 8569 | MKNK1 | MAPK interacting serine/thr... | XM_006711003.1 | 88.8% | 37.8% | (many diffs) |
| 6 | human | 8569 | MKNK1 | MAPK interacting serine/thr... | NM_001135553.4 | 87.8% | 37.6% | (many diffs) |
| 7 | human | 8569 | MKNK1 | MAPK interacting serine/thr... | XM_024450508.1 | 87.8% | 37.6% | (many diffs) |
| 8 | human | 8569 | MKNK1 | MAPK interacting serine/thr... | XM_024450509.1 | 87.8% | 37.6% | (many diffs) |
| 9 | human | 8569 | MKNK1 | MAPK interacting serine/thr... | XM_024450510.1 | 87.8% | 37.6% | (many diffs) |
| 10 | human | 8569 | MKNK1 | MAPK interacting serine/thr... | XM_006711007.1 | 78.6% | 23.9% | (many diffs) |
| 11 | human | 8569 | MKNK1 | MAPK interacting serine/thr... | XM_024450511.1 | 78.6% | 23.9% | (many diffs) |
| 12 | human | 8569 | MKNK1 | MAPK interacting serine/thr... | XM_017002652.2 | 77% | 24.8% | (many diffs) |
| 13 | human | 8569 | MKNK1 | MAPK interacting serine/thr... | XM_017002653.1 | 77% | 24.8% | (many diffs) |
| 14 | human | 8569 | MKNK1 | MAPK interacting serine/thr... | XM_017002659.1 | 73.8% | 49% | (many diffs) |
| 15 | human | 8569 | MKNK1 | MAPK interacting serine/thr... | XM_017002660.2 | 72.4% | 45.9% | (many diffs) |
| 16 | human | 8569 | MKNK1 | MAPK interacting serine/thr... | XM_017002661.2 | 72.4% | 45.9% | (many diffs) |
| 17 | human | 8569 | MKNK1 | MAPK interacting serine/thr... | XM_017002662.1 | 72.4% | 45.9% | (many diffs) |
| 18 | human | 8569 | MKNK1 | MAPK interacting serine/thr... | NM_198973.5 | 71.3% | 45.7% | (many diffs) |
| 19 | human | 8569 | MKNK1 | MAPK interacting serine/thr... | XM_024450512.1 | 71.3% | 45.7% | (many diffs) |
| 20 | human | 8569 | MKNK1 | MAPK interacting serine/thr... | XM_024450513.1 | 71.3% | 45.7% | (many diffs) |
| 21 | human | 8569 | MKNK1 | MAPK interacting serine/thr... | XM_024450514.1 | 71.3% | 45.7% | (many diffs) |
| 22 | human | 8569 | MKNK1 | MAPK interacting serine/thr... | XM_006711013.1 | 70% | 13% | (many diffs) |
| 23 | human | 8569 | MKNK1 | MAPK interacting serine/thr... | XM_006711014.1 | 70% | 13% | (many diffs) |
| 24 | human | 8569 | MKNK1 | MAPK interacting serine/thr... | XM_006711015.1 | 70% | 13% | (many diffs) |
| 25 | human | 8569 | MKNK1 | MAPK interacting serine/thr... | NR_146512.2 | 43.9% | (many diffs) | |
| 26 | human | 8569 | MKNK1 | MAPK interacting serine/thr... | NR_024176.3 | 41.2% | (many diffs) | |
| 27 | human | 8569 | MKNK1 | MAPK interacting serine/thr... | NR_024174.3 | 41.1% | (many diffs) | |
| 28 | mouse | 17346 | Mknk1 | MAP kinase-interacting seri... | NM_001285487.1 | 77.4% | 34.9% | (many diffs) |
| 29 | mouse | 17346 | Mknk1 | MAP kinase-interacting seri... | XM_006502822.3 | 77.4% | 34.9% | (many diffs) |
| 30 | mouse | 17346 | Mknk1 | MAP kinase-interacting seri... | XM_006502823.3 | 77.1% | 35.1% | (many diffs) |
| 31 | mouse | 17346 | Mknk1 | MAP kinase-interacting seri... | NM_001285488.1 | 76.4% | 34.6% | (many diffs) |
| 32 | mouse | 17346 | Mknk1 | MAP kinase-interacting seri... | NM_021461.5 | 76.4% | 34.6% | (many diffs) |
| 33 | mouse | 17346 | Mknk1 | MAP kinase-interacting seri... | XM_006502825.3 | 64.6% | 42.6% | (many diffs) |
| 34 | mouse | 17346 | Mknk1 | MAP kinase-interacting seri... | XM_017320015.1 | 63.5% | 42.3% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 696
- ORF length:
- 630
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggt atcttctcaa aagttggaaa aacctataga gatgggcagt agcgaacccc 121 ttcccatcgc agatggtgac aggaggagga agaagaagcg gaggggccgg gccactgact 181 ccttgccagg aaagtttgaa gatatgtaca agctgacctc tgaattgctt ggagagggag 241 cctatgccaa agttcaaggt gccgtgagcc tacagaatgg caaagagtat gccgtcaaaa 301 tcatcgagaa acaagcaggg cacagtcgga gtagggtgtt tcgagaggtg gagacgctgt 361 atcagtgtca gggaaacaag aacattttgg agctgattga gttctttgaa gatgacacaa 421 ggttttactt ggtctttgag aaattgcaag gaggttccat cttagcccac atccagaagc 481 aaaagcactt caatgagcga gaagccagcc gagtggtgcg ggacgttgct gctgcccttg 541 acttcctgca taccaaagac aaagtctctc tctgtcacct aggctggagt gctatggcgc 601 catcagggct cactgcagcc ccaacctccc tgggctccag tgatcctccc acctcagcct 661 cccaagtagc tgggactaca ngcatttgct catngtgatn tgaaaccaag naaaatatat 721 tgtgtgaatc tccaggaaaa ggtgtctcca gtgaaaaatc tgtgactttg acttgggcag 781 tgggatgaaa ctgaacaact ccttgtaccc ccataaccac accagagctg accaccccat 841 gtggctctgc agaatacatg gcccctgaag gtagtngagg tcttcacgga ccaggccaca 901 ttctacgaca agcgctgtga cctgtggagc ctgggcgtgg tcctctacat catgctgagt 961 ggctacccac ccttcgtggg tcactgcggg gccgactgtg gctgggaccg gggcgaggtc 1021 tgcagggtgt gccagaacaa gctgtttgaa agcatccagg aaggCAAGTA TGAGTTTCCT 1081 GACAAGGACT GGGCACACAT CTCCAGTGAA GCCAAAGACC TCATCTCCAA GCTCCTGGTG 1141 CGAGATGCAA AGCAGAGACT TAGCGCCGCC CAAGTTCTGC AGCACCCATG GGTGCAGGGG 1201 CAAGCTCCAG AAAAGGGACT CCCCACGCCG CAAGTCCTCC AGAGGAACAG CAGCACAATG 1261 GACCTGACGC TCTTCGCAGC TGAGGCCATC GCCCTTAACC GCCAGCTATC TCAGCACGAA 1321 GAGAACGAAC TAGCAGAGGA GCCAGAGGCA CTAGCTGATG GCCTCTGCTC CATGAAGCTT 1381 TCCCCTCCCT GCAAGTCACG CCTGGCCCGG AGACGGGCCC TGGCCCAGGC AGGCCGTGGT 1441 GAAGACAGGA GCCCGCCCAC AGCACTCTGC CCAACTTTCT TGTACAAAGT GGTTGATATC 1501 GGTAAGCCTA TCCCTAACCC TCTCCTCGGT CTCGATTCTA CGTAGTAATG AACTAGTCCG 1561 TAACTTGAAA GTATTTCGAT TTCTTGGCTT TATATATCTT GTGGAAAGGA CGAAATATAT 1621 GGTTTCTGAT GTGGAATACG CGTTAAGTCg acaatcaacc tctggattac aaaatttgtg 1681 aaagatt