Transcript: Human NM_001256649.2

Homo sapiens zinc finger protein 43 (ZNF43), transcript variant 2, mRNA.

Source:
NCBI, updated 2019-06-02
Taxon:
Homo sapiens (human)
Gene:
ZNF43 (7594)
Length:
5636
CDS:
564..2975

Additional Resources:

NCBI RefSeq record:
NM_001256649.2
NBCI Gene record:
ZNF43 (7594)

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human NM_001256649.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000218423 ATTAATGCTCGTACCTTATTG pLKO_005 3111 3UTR 100% 13.200 18.480 N ZNF43 n/a
2 TRCN0000234363 CCACATCTAGCTCAACATAAA pLKO_005 1104 CDS 100% 13.200 9.240 N ZNF43 n/a
3 TRCN0000147152 CTGAAGATGTGACAGTGATTT pLKO.1 2920 CDS 100% 13.200 9.240 N ZNF43 n/a
4 TRCN0000234365 CTGAAGATGTGACAGTGATTT pLKO_005 2920 CDS 100% 13.200 9.240 N ZNF43 n/a
5 TRCN0000234364 TGCCCTTCAATCATCACTAAA pLKO_005 1182 CDS 100% 13.200 9.240 N ZNF43 n/a
6 TRCN0000234362 GCCTATGAGGAGACATGAAAT pLKO_005 737 CDS 100% 13.200 7.920 N ZNF43 n/a
7 TRCN0000147126 CCCAGTTATGTGTTCTCATTT pLKO.1 770 CDS 100% 13.200 6.600 Y ZNF43 n/a
8 TRCN0000096537 CTGGAGAGAAACCCTACAAAT pLKO.1 1807 CDS 100% 13.200 6.600 Y Zfp934 n/a
9 TRCN0000235358 CTGGAGAGAAACCCTACAAAT pLKO_005 1807 CDS 100% 13.200 6.600 Y 2810408B13Rik n/a
10 TRCN0000244342 CTGGAGAGAAACCCTACAAAT pLKO_005 1807 CDS 100% 13.200 6.600 Y EG668616 n/a
11 TRCN0000107875 CACTTGATTGTAGGTAAGATA pLKO.1 3038 3UTR 100% 5.625 2.813 Y ZNF254 n/a
12 TRCN0000146802 CCTCAAACCTTACTACACATA pLKO.1 2278 CDS 100% 4.950 2.475 Y ZNF714 n/a
13 TRCN0000016587 CCTGGGTATTGCTGTCTCTAA pLKO.1 671 CDS 100% 4.950 2.475 Y ZNF675 n/a
14 TRCN0000168774 GAGATGGAGTTTCACCATGTT pLKO.1 5285 3UTR 100% 4.950 2.475 Y LOC400464 n/a
15 TRCN0000018502 GATGTTAGAGAACTACAGAAA pLKO.1 641 CDS 100% 4.950 2.475 Y ZNF493 n/a
16 TRCN0000107758 TGTCTCTAAGCCAGACCTGAT pLKO.1 683 CDS 100% 4.050 2.025 Y ZNF273 n/a
17 TRCN0000018036 CCCAGTTATGTGTTCTCATAT pLKO.1 770 CDS 100% 13.200 6.600 Y ZNF257 n/a
18 TRCN0000107291 CCCTTTCTACACATAAGATAA pLKO.1 2620 CDS 100% 13.200 6.600 Y ZNF66 n/a
19 TRCN0000136653 GCCTGACCAACATGGTGAAAT pLKO.1 4205 3UTR 100% 13.200 6.600 Y IQCC n/a
20 TRCN0000179120 CAACATGGTGAAACCCTGTTT pLKO.1 4212 3UTR 100% 4.950 2.475 Y LOC339059 n/a
21 TRCN0000158848 GAGAAACCTTACAAATGTGAT pLKO.1 1476 CDS 100% 4.950 2.475 Y ZNF28 n/a
Download CSV

shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript NM_001256649.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

Download CSV

All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_07157 pDONR223 100% 99.2% 99.1% None 0_1insATGGGACCATTAACATTT;670T>C n/a
2 ccsbBroad304_07157 pLX_304 0% 99.2% 99.1% V5 0_1insATGGGACCATTAACATTT;670T>C n/a
3 TRCN0000475452 TAAAACTTCAACTTGGTTTCCTTC pLX_317 10.2% 99.2% 99.1% V5 0_1insATGGGACCATTAACATTT;670T>C n/a
4 ccsbBroadEn_02188 pDONR223 100% 67.4% 57.4% None (many diffs) n/a
5 ccsbBroad304_02188 pLX_304 0% 67.4% 57.4% V5 (many diffs) n/a
6 TRCN0000476436 TCCGTGTTGGTCAAGCGACGCGCC pLX_317 12.5% 67.4% 57.4% V5 (many diffs) n/a
7 ccsbBroadEn_15278 pDONR223 59.5% 62.3% 52.7% None (many diffs) n/a
8 ccsbBroad304_15278 pLX_304 0% 62.3% 52.7% V5 (many diffs) n/a
9 ccsbBroadEn_09655 pDONR223 100% 56.4% 48.6% None (many diffs) n/a
10 ccsbBroad304_09655 pLX_304 0% 56.4% 48.6% V5 (many diffs) n/a
11 TRCN0000468487 GAACACCAGATCGGTGGCCCTCAA pLX_317 20.1% 56.4% 48.6% V5 (many diffs) n/a
12 ccsbBroadEn_11232 pDONR223 100% 55.8% 48% None (many diffs) n/a
13 ccsbBroad304_11232 pLX_304 0% 55.8% 48% V5 (many diffs) n/a
14 TRCN0000476048 TACTATCCCAATAGAAACACCTCC pLX_317 21.8% 55.8% 48% V5 (many diffs) n/a
15 ccsbBroadEn_09774 pDONR223 100% 55.8% 46.8% None (many diffs) n/a
16 ccsbBroad304_09774 pLX_304 0% 55.8% 46.8% V5 (many diffs) n/a
17 TRCN0000472815 CCTGACTCCCTTTAAGTGTTCGTG pLX_317 27.4% 55.8% 46.8% V5 (many diffs) n/a
18 ccsbBroadEn_09784 pDONR223 100% 55.8% 46.9% None (many diffs) n/a
19 ccsbBroad304_09784 pLX_304 0% 55.8% 46.9% V5 (many diffs) n/a
20 TRCN0000478115 ATTTTTATATATACCACTCGGCCC pLX_317 19.7% 55.8% 46.9% V5 (many diffs) n/a
21 ccsbBroadEn_10024 pDONR223 100% 54.9% 45.9% None (many diffs) n/a
22 ccsbBroad304_10024 pLX_304 0% 54.9% 45.9% V5 (many diffs) n/a
23 TRCN0000466950 AAAAATGGGCGCTCTGAGACACAC pLX_317 21.1% 54.9% 45.9% V5 (many diffs) n/a
24 ccsbBroadEn_15167 pDONR223 53.6% 54.2% 20.7% None (many diffs) n/a
25 ccsbBroad304_15167 pLX_304 0% 54.2% 20.7% V5 (not translated due to prior stop codon) (many diffs) n/a
26 ccsbBroadEn_08635 pDONR223 100% 51.4% 43% None (many diffs) n/a
27 ccsbBroad304_08635 pLX_304 0% 51.4% 43% V5 (many diffs) n/a
28 TRCN0000469248 TACCCGCTTGGCTTTAAAAACCAA pLX_317 37.7% 40.7% 33% V5 (many diffs) n/a
29 ccsbBroadEn_15273 pDONR223 50.9% 51.2% 42.4% None (many diffs) n/a
30 ccsbBroad304_15273 pLX_304 0% 51.2% 42.4% V5 (many diffs) n/a
31 TRCN0000472761 GCGGTTCAATGTTGTAGTCTTGTG pLX_317 63.8% 22.4% 18.1% V5 (not translated due to frame shift) (many diffs) n/a
32 ccsbBroadEn_15729 pDONR223 0% 8.4% 7.2% None (many diffs) n/a
33 ccsbBroad304_15729 pLX_304 0% 8.4% 7.2% V5 (many diffs) n/a
34 TRCN0000470492 GCCGACTTGCTCCATGATGCAGCT pLX_317 100% 8.4% 7.2% V5 (many diffs) n/a
35 ccsbBroadEn_11384 pDONR223 100% 8.2% 7.3% None (many diffs) n/a
36 ccsbBroad304_11384 pLX_304 0% 8.2% 7.3% V5 (many diffs) n/a
37 TRCN0000470576 TACATACAGACCTACACGTAGACC pLX_317 100% 8.2% 7.3% V5 (many diffs) n/a
38 ccsbBroadEn_13746 pDONR223 100% 7.8% 7% None (many diffs) n/a
39 ccsbBroad304_13746 pLX_304 0% 7.8% 7% V5 (many diffs) n/a
40 TRCN0000475669 ATTCGCAGCGAGTTTGTCCGCCGC pLX_317 100% 7.8% 7% V5 (many diffs) n/a
Download CSV

GPP Web Portal Terms of Service

Effective Date: December 8, 2025
By using this site, you agree to our terms and conditions below.

Overview of Terms

The data made available on this website were generated for research purposes and are not intended for clinical or commercial uses. Commercial use (or other use for profit-making purposes) of the GPP Web Portal and its tools, is not permitted under these terms and may require a separate license agreement from Broad or its contributors. For more information, please contact partnering@broadinstitute.org.

The original data may be subject to rights claimed by third parties, including but not limited to, patent, copyright, other intellectual property rights, biodiversity-related access and benefit-sharing rights. It is the responsibility of users of Broad Institute services to ensure that their use of the data does not infringe any of the rights of such third parties.

Any questions or comments concerning these Terms of Use can be addressed to: legal@broadinstitute.org.

By accessing and viewing this GPP Web Portal, you agree to the following terms and conditions:

Attribution

You agree to acknowledge the Broad Institute (e.g., in publications, services or products) for any of your use of its online services, databases or software in accordance with good scientific practice. You agree to use the acknowledgment wording provided for the relevant tools as indicated on the FAQ for each tool.

Updating the Terms of Use

We reserve the right to update these Terms of Use at any time. When alterations are inevitable, we will attempt to give reasonable notice of any changes by placing a notice on our website, but you may wish to check each time you use the website. The date of the most recent revision will appear on this, the "GPP Web Portal Terms of Use" page. If you do not agree to these changes, please do not continue to use our online services. We will also make available an archived copy of the previous Terms of Use for comparison.

Indemnification and Disclaimer of Warranties

You are using this GPP Web Portal at your own risk, and you hereby agree to hold Broad and its contributors and their trustees, directors, officers, employees, and affiliated investigators harmless for any third party claims which may arise from your use of the GPP Web Portal, the tools available therein, or any portion thereof. Further, you agree to indemnify Broad, its contributors, and its and their trustees, directors, officers, employees, affiliated investigators, students, and affiliates for any loss, costs, claims, damages, or other liabilities arising from any unpermitted commercial or profit-making use you make of the GPP Web Portal. The GPP Web Portal is a research tool and is provided "as is". Broad does not represent that the GPP Web Portal is free of errors or bugs or suitable for any particular tasks.

ANY EXPRESS OR IMPLIED WARRANTIES, INCLUDING, BUT NOT LIMITED TO, THE IMPLIED WARRANTIES OF MERCHANTABILITY, FITNESS FOR A PARTICULAR PURPOSE, NONINFRINGEMENT, OR THE ABSENCE OF LATENT OR OTHER DEFECTS ARE DISCLAIMED. IN NO EVENT SHALL BROAD OR ITS CONTRIBUTORS BE LIABLE FOR ANY DIRECT, INDIRECT, INCIDENTAL, SPECIAL, EXEMPLARY, OR CONSEQUENTIAL DAMAGES HOWEVER CAUSED AND ON ANY THEORY OF LIABILITY, WHETHER IN CONTRACT, STRICT LIABILITY, OR TORT (INCLUDING NEGLIGENCE OR OTHERWISE) ARISING IN ANY WAY OUT OF THE USE OF THE GPP WEB PORTAL, EVEN IF ADVISED OF THE POSSIBILITY OF SUCH DAMAGE.

Governing Law

The terms and conditions herein shall be construed, governed, interpreted, and applied in accordance with the internal laws of the Commonwealth of Massachusetts, U.S.A. Furthermore, by accessing, downloading, or using the Database, You consent to the personal jurisdiction of, and venue in, the state and federal courts within Massachusetts with respect to Your download or use of the Database.