Transcript: Human NM_021269.3

Homo sapiens zinc finger protein 708 (ZNF708), transcript variant 1, mRNA.

Source:
NCBI, updated 2019-09-27
Taxon:
Homo sapiens (human)
Gene:
ZNF708 (7562)
Length:
4004
CDS:
199..1890

Additional Resources:

NCBI RefSeq record:
NM_021269.3
NBCI Gene record:
ZNF708 (7562)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human NM_021269.3, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000164774 CCTGGGTATTGCTGTGTCTAA pLKO.1 324 CDS 100% 4.950 6.930 N ZNF708 n/a
2 TRCN0000239659 AGGAGGTCACAAGGGACTTAA pLKO_005 564 CDS 100% 13.200 9.240 N ZNF708 n/a
3 TRCN0000158564 CCCAAACTAACGGTAGATAAA pLKO.1 3186 3UTR 100% 13.200 9.240 N ZNF708 n/a
4 TRCN0000239658 GACCTTAGGCCAGAGCAATAT pLKO_005 448 CDS 100% 13.200 9.240 N ZNF708 n/a
5 TRCN0000159275 GCTTCAGACATTACACTATAT pLKO.1 3140 3UTR 100% 13.200 9.240 N ZNF708 n/a
6 TRCN0000239657 GGATATCAGAAAGGCTGTAAA pLKO_005 517 CDS 100% 13.200 9.240 N ZNF708 n/a
7 TRCN0000239660 TCTAAGTGGAGATGGTATTTG pLKO_005 3821 3UTR 100% 13.200 9.240 N ZNF708 n/a
8 TRCN0000239656 TATTCTGTCCTCTCATCTTAC pLKO_005 1653 CDS 100% 10.800 7.560 N ZNF708 n/a
9 TRCN0000159621 GCATCAAAGATAGGAGAGTTT pLKO.1 3720 3UTR 100% 4.950 3.465 N ZNF708 n/a
10 TRCN0000012931 CCCAGCTATGTGTTCTCATTT pLKO.1 420 CDS 100% 13.200 6.600 Y ZNF141 n/a
11 TRCN0000160536 CTCAACCCTTATGAAACATAA pLKO.1 1746 CDS 100% 13.200 6.600 Y ZNF708 n/a
12 TRCN0000428574 CTGAAGAGAAACCCTACAAAT pLKO_005 1274 CDS 100% 13.200 6.600 Y ZNF138 n/a
13 TRCN0000243748 CTGGAAAGAAACCCTACAAAT pLKO_005 1442 CDS 100% 13.200 6.600 Y Gm6871 n/a
14 TRCN0000096537 CTGGAGAGAAACCCTACAAAT pLKO.1 854 CDS 100% 13.200 6.600 Y Zfp934 n/a
15 TRCN0000235358 CTGGAGAGAAACCCTACAAAT pLKO_005 854 CDS 100% 13.200 6.600 Y 2810408B13Rik n/a
16 TRCN0000244342 CTGGAGAGAAACCCTACAAAT pLKO_005 854 CDS 100% 13.200 6.600 Y EG668616 n/a
17 TRCN0000107875 CACTTGATTGTAGGTAAGATA pLKO.1 2253 3UTR 100% 5.625 2.813 Y ZNF254 n/a
18 TRCN0000218427 ACTGGAGAGAAACCCTATAAA pLKO_005 1609 CDS 100% 15.000 7.500 Y ZNF443 n/a
19 TRCN0000374174 ACTGGAGAGAAACCCTATAAA pLKO_005 1609 CDS 100% 15.000 7.500 Y Zfp97 n/a
20 TRCN0000017702 CCCTGGAATATGAAGAGACAT pLKO.1 382 CDS 100% 4.950 2.475 Y ZNF430 n/a
21 TRCN0000148469 CTGGGTTCAAGCAATTCTCTT pLKO.1 3373 3UTR 100% 4.950 2.475 Y C16orf89 n/a
22 TRCN0000149073 GCAAAGCCTTTAACCAGTCTT pLKO.1 1811 CDS 100% 4.950 2.475 Y ZNF714 n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript NM_021269.3, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_15278 pDONR223 59.5% 81.9% 71.5% None (many diffs) n/a
2 ccsbBroad304_15278 pLX_304 0% 81.9% 71.5% V5 (many diffs) n/a
3 ccsbBroadEn_09784 pDONR223 100% 77.4% 66% None (many diffs) n/a
4 ccsbBroad304_09784 pLX_304 0% 77.4% 66% V5 (many diffs) n/a
5 TRCN0000478115 ATTTTTATATATACCACTCGGCCC pLX_317 19.7% 77.4% 66% V5 (many diffs) n/a
6 ccsbBroadEn_13028 pDONR223 100% 77.2% 67.4% None (many diffs) n/a
7 ccsbBroad304_13028 pLX_304 0% 77.2% 67.4% V5 (many diffs) n/a
8 TRCN0000468257 GTACACCAGACCACTACATGCGAC pLX_317 25.6% 77.2% 67.4% V5 (many diffs) n/a
9 ccsbBroadEn_15167 pDONR223 53.6% 76.1% 31.7% None (many diffs) n/a
10 ccsbBroad304_15167 pLX_304 0% 76.1% 31.7% V5 (not translated due to prior stop codon) (many diffs) n/a
11 ccsbBroadEn_10024 pDONR223 100% 75.1% 63.4% None (many diffs) n/a
12 ccsbBroad304_10024 pLX_304 0% 75.1% 63.4% V5 (many diffs) n/a
13 TRCN0000466950 AAAAATGGGCGCTCTGAGACACAC pLX_317 21.1% 75.1% 63.4% V5 (many diffs) n/a
14 ccsbBroadEn_11550 pDONR223 100% 74.4% 63.7% None (many diffs) n/a
15 ccsbBroad304_11550 pLX_304 0% 74.4% 63.7% V5 (many diffs) n/a
16 ccsbBroadEn_08635 pDONR223 100% 73.5% 62.8% None (many diffs) n/a
17 ccsbBroad304_08635 pLX_304 0% 73.5% 62.8% V5 (many diffs) n/a
18 TRCN0000469248 TACCCGCTTGGCTTTAAAAACCAA pLX_317 37.7% 57.1% 47.7% V5 (many diffs) n/a
19 ccsbBroadEn_02188 pDONR223 100% 72.9% 64.3% None (many diffs) n/a
20 ccsbBroad304_02188 pLX_304 0% 72.9% 64.3% V5 (many diffs) n/a
21 TRCN0000476436 TCCGTGTTGGTCAAGCGACGCGCC pLX_317 12.5% 72.9% 64.3% V5 (many diffs) n/a
22 ccsbBroadEn_15273 pDONR223 50.9% 72.2% 61.8% None (many diffs) n/a
23 ccsbBroad304_15273 pLX_304 0% 72.2% 61.8% V5 (many diffs) n/a
24 TRCN0000472761 GCGGTTCAATGTTGTAGTCTTGTG pLX_317 63.8% 31.5% 26.5% V5 (not translated due to frame shift) (many diffs) n/a
25 ccsbBroadEn_09774 pDONR223 100% 71.8% 61.7% None (many diffs) n/a
26 ccsbBroad304_09774 pLX_304 0% 71.8% 61.7% V5 (many diffs) n/a
27 TRCN0000472815 CCTGACTCCCTTTAAGTGTTCGTG pLX_317 27.4% 71.8% 61.7% V5 (many diffs) n/a
28 ccsbBroadEn_07157 pDONR223 100% 60.6% 53.8% None (many diffs) n/a
29 ccsbBroad304_07157 pLX_304 0% 60.6% 53.8% V5 (many diffs) n/a
30 TRCN0000475452 TAAAACTTCAACTTGGTTTCCTTC pLX_317 10.2% 60.6% 53.8% V5 (many diffs) n/a
31 ccsbBroadEn_15729 pDONR223 0% 12.7% 11.8% None (many diffs) n/a
32 ccsbBroad304_15729 pLX_304 0% 12.7% 11.8% V5 (many diffs) n/a
33 TRCN0000470492 GCCGACTTGCTCCATGATGCAGCT pLX_317 100% 12.7% 11.8% V5 (many diffs) n/a
34 ccsbBroadEn_11384 pDONR223 100% 12.3% 11.3% None (many diffs) n/a
35 ccsbBroad304_11384 pLX_304 0% 12.3% 11.3% V5 (many diffs) n/a
36 TRCN0000470576 TACATACAGACCTACACGTAGACC pLX_317 100% 12.3% 11.3% V5 (many diffs) n/a
37 ccsbBroadEn_13746 pDONR223 100% 11.9% 11% None (many diffs) n/a
38 ccsbBroad304_13746 pLX_304 0% 11.9% 11% V5 (many diffs) n/a
39 TRCN0000475669 ATTCGCAGCGAGTTTGTCCGCCGC pLX_317 100% 11.9% 11% V5 (many diffs) n/a
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