Transcript: Human NM_021148.2

Homo sapiens zinc finger protein 273 (ZNF273), transcript variant 1, mRNA.

Source:
NCBI, updated 2019-05-04
Taxon:
Homo sapiens (human)
Gene:
ZNF273 (10793)
Length:
4325
CDS:
77..1786

Additional Resources:

NCBI RefSeq record:
NM_021148.2
NBCI Gene record:
ZNF273 (10793)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human NM_021148.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000108014 TGCTACTAGAGTGAATTTCTA pLKO.1 757 CDS 100% 5.625 3.938 N ZNF273 n/a
2 TRCN0000108011 TCCTGAAGTGAATCCCTACAA pLKO.1 844 CDS 100% 4.950 3.465 N ZNF273 n/a
3 TRCN0000108013 CCAGTCCTTAACTCTTACTAA pLKO.1 892 CDS 100% 5.625 3.375 N ZNF273 n/a
4 TRCN0000107756 CCCTGCAATATGAAGAGACAT pLKO.1 359 CDS 100% 4.950 2.970 N ZNF273 n/a
5 TRCN0000108010 GCTGCATCAAAGATATGAGAT pLKO.1 2966 3UTR 100% 4.950 2.970 N ZNF273 n/a
6 TRCN0000107759 AGACATGCGATGGTAGCCAAA pLKO.1 374 CDS 100% 4.050 2.430 N ZNF273 n/a
7 TRCN0000108012 GAGAGAAACCATACAAACCTA pLKO.1 1689 CDS 100% 3.000 1.800 N ZNF273 n/a
8 TRCN0000107757 CAATATGAAGAGACATGCGAT pLKO.1 364 CDS 100% 2.640 1.584 N ZNF273 n/a
9 TRCN0000096537 CTGGAGAGAAACCCTACAAAT pLKO.1 1098 CDS 100% 13.200 6.600 Y Zfp934 n/a
10 TRCN0000235358 CTGGAGAGAAACCCTACAAAT pLKO_005 1098 CDS 100% 13.200 6.600 Y 2810408B13Rik n/a
11 TRCN0000244342 CTGGAGAGAAACCCTACAAAT pLKO_005 1098 CDS 100% 13.200 6.600 Y EG668616 n/a
12 TRCN0000016587 CCTGGGTATTGCTGTCTCTAA pLKO.1 301 CDS 100% 4.950 2.475 Y ZNF675 n/a
13 TRCN0000107758 TGTCTCTAAGCCAGACCTGAT pLKO.1 313 CDS 100% 4.050 2.025 Y ZNF273 n/a
14 TRCN0000344452 CCCTTACTAGACATAAGATAA pLKO_005 1239 CDS 100% 13.200 6.600 Y ZNF737 n/a
15 TRCN0000158848 GAGAAACCTTACAAATGTGAT pLKO.1 935 CDS 100% 4.950 2.475 Y ZNF28 n/a
16 TRCN0000149073 GCAAAGCCTTTAACCAGTCTT pLKO.1 879 CDS 100% 4.950 2.475 Y ZNF714 n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript NM_021148.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_11550 pDONR223 100% 88.5% 88.5% None 1_195del n/a
2 ccsbBroad304_11550 pLX_304 0% 88.5% 88.5% V5 1_195del n/a
3 ccsbBroadEn_10024 pDONR223 100% 80.3% 70.6% None (many diffs) n/a
4 ccsbBroad304_10024 pLX_304 0% 80.3% 70.6% V5 (many diffs) n/a
5 TRCN0000466950 AAAAATGGGCGCTCTGAGACACAC pLX_317 21.1% 80.3% 70.6% V5 (many diffs) n/a
6 ccsbBroadEn_15167 pDONR223 53.6% 79.2% 37.3% None (many diffs) n/a
7 ccsbBroad304_15167 pLX_304 0% 79.2% 37.3% V5 (not translated due to prior stop codon) (many diffs) n/a
8 ccsbBroadEn_15278 pDONR223 59.5% 74.5% 64.7% None (many diffs) n/a
9 ccsbBroad304_15278 pLX_304 0% 74.5% 64.7% V5 (many diffs) n/a
10 ccsbBroadEn_08635 pDONR223 100% 72.6% 59.7% None (many diffs) n/a
11 ccsbBroad304_08635 pLX_304 0% 72.6% 59.7% V5 (many diffs) n/a
12 TRCN0000469248 TACCCGCTTGGCTTTAAAAACCAA pLX_317 37.7% 58.4% 48.5% V5 (many diffs) n/a
13 ccsbBroadEn_09302 pDONR223 100% 68.9% 57.6% None (many diffs) n/a
14 ccsbBroad304_09302 pLX_304 0% 68.9% 57.6% V5 (many diffs) n/a
15 TRCN0000478136 TCTGGATTCCTTTAAAAGGATTTC pLX_317 23% 68.9% 57.6% V5 (many diffs) n/a
16 ccsbBroadEn_02188 pDONR223 100% 67.8% 59% None (many diffs) n/a
17 ccsbBroad304_02188 pLX_304 0% 67.8% 59% V5 (many diffs) n/a
18 TRCN0000476436 TCCGTGTTGGTCAAGCGACGCGCC pLX_317 12.5% 67.8% 59% V5 (many diffs) n/a
19 ccsbBroadEn_09774 pDONR223 100% 64.5% 55.4% None (many diffs) n/a
20 ccsbBroad304_09774 pLX_304 0% 64.5% 55.4% V5 (many diffs) n/a
21 TRCN0000472815 CCTGACTCCCTTTAAGTGTTCGTG pLX_317 27.4% 64.5% 55.4% V5 (many diffs) n/a
22 ccsbBroadEn_07157 pDONR223 100% 53.7% 46.3% None (many diffs) n/a
23 ccsbBroad304_07157 pLX_304 0% 53.7% 46.3% V5 (many diffs) n/a
24 TRCN0000475452 TAAAACTTCAACTTGGTTTCCTTC pLX_317 10.2% 53.7% 46.3% V5 (many diffs) n/a
25 ccsbBroadEn_11384 pDONR223 100% 14.1% 13% None (many diffs) n/a
26 ccsbBroad304_11384 pLX_304 0% 14.1% 13% V5 (many diffs) n/a
27 TRCN0000470576 TACATACAGACCTACACGTAGACC pLX_317 100% 14.1% 13% V5 (many diffs) n/a
28 ccsbBroadEn_15729 pDONR223 0% 13.9% 13.1% None (many diffs) n/a
29 ccsbBroad304_15729 pLX_304 0% 13.9% 13.1% V5 (many diffs) n/a
30 TRCN0000470492 GCCGACTTGCTCCATGATGCAGCT pLX_317 100% 13.9% 13.1% V5 (many diffs) n/a
31 ccsbBroadEn_13746 pDONR223 100% 12% 10.8% None (many diffs) n/a
32 ccsbBroad304_13746 pLX_304 0% 12% 10.8% V5 (many diffs) n/a
33 TRCN0000475669 ATTCGCAGCGAGTTTGTCCGCCGC pLX_317 100% 12% 10.8% V5 (many diffs) n/a
34 ccsbBroadEn_11549 pDONR223 100% 8.3% 7.6% None (many diffs) n/a
35 ccsbBroad304_11549 pLX_304 94.6% 8.3% 7.6% V5 (many diffs) n/a
36 TRCN0000468281 AACATTAGGAAAGAACCCCCACCC pLX_317 100% 8.3% 7.6% V5 (many diffs) n/a
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