Construct: ORF TRCN0000488694
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF022026.1_s317c1
- DNA Barcode:
- TTCTCCCTGACTACGATACCCGTC
- Epitope Tag:
- V5 (not translated due to prior stop codon)
- Notes:
- Early stop codon detected
Originally Annotated References:
- Gene:
- RIC3 (79608)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000488694
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 79608 | RIC3 | RIC3 acetylcholine receptor... | NM_024557.6 | 99.7% | 68.7% | 170C>A;493A>T;763_764insT |
| 2 | human | 79608 | RIC3 | RIC3 acetylcholine receptor... | XM_006718318.4 | 99.7% | 68.7% | 170C>A;493A>T;763_764insT |
| 3 | human | 79608 | RIC3 | RIC3 acetylcholine receptor... | NM_001206671.4 | 99.4% | 68.5% | (many diffs) |
| 4 | human | 79608 | RIC3 | RIC3 acetylcholine receptor... | XM_006718317.4 | 92.4% | 63.7% | (many diffs) |
| 5 | human | 79608 | RIC3 | RIC3 acetylcholine receptor... | XM_017018287.2 | 92.4% | 63.7% | (many diffs) |
| 6 | human | 79608 | RIC3 | RIC3 acetylcholine receptor... | NM_001346692.2 | 86.4% | 55.4% | (many diffs) |
| 7 | human | 79608 | RIC3 | RIC3 acetylcholine receptor... | XM_024448684.1 | 86.4% | 55.4% | (many diffs) |
| 8 | human | 79608 | RIC3 | RIC3 acetylcholine receptor... | NM_001346694.2 | 86.1% | 55.2% | (many diffs) |
| 9 | human | 79608 | RIC3 | RIC3 acetylcholine receptor... | NM_001346693.2 | 80.1% | 51.3% | (many diffs) |
| 10 | human | 79608 | RIC3 | RIC3 acetylcholine receptor... | NM_001206672.4 | 78% | 47% | 170C>A;427_428ins240;523_524insT |
| 11 | human | 79608 | RIC3 | RIC3 acetylcholine receptor... | XR_930900.3 | 63.3% | (many diffs) | |
| 12 | human | 79608 | RIC3 | RIC3 acetylcholine receptor... | NM_001346690.2 | 54.3% | 23% | (many diffs) |
| 13 | human | 79608 | RIC3 | RIC3 acetylcholine receptor... | NM_001135109.4 | 50.7% | 20.1% | 120_121ins543;220_221insT |
| 14 | human | 79608 | RIC3 | RIC3 acetylcholine receptor... | XM_006718321.4 | 50.4% | 64.6% | (many diffs) |
| 15 | human | 79608 | RIC3 | RIC3 acetylcholine receptor... | XM_024448685.1 | 48.3% | 67.7% | (many diffs) |
| 16 | human | 79608 | RIC3 | RIC3 acetylcholine receptor... | XM_024448686.1 | 48.3% | 67.7% | (many diffs) |
| 17 | human | 79608 | RIC3 | RIC3 acetylcholine receptor... | NM_001346691.2 | 47.5% | 66.9% | (many diffs) |
| 18 | human | 79608 | RIC3 | RIC3 acetylcholine receptor... | XR_001747957.2 | 43.5% | (many diffs) | |
| 19 | human | 79608 | RIC3 | RIC3 acetylcholine receptor... | XR_428851.4 | 42.1% | (many diffs) | |
| 20 | human | 79608 | RIC3 | RIC3 acetylcholine receptor... | NR_144485.2 | 41.6% | (many diffs) | |
| 21 | human | 79608 | RIC3 | RIC3 acetylcholine receptor... | XR_930896.3 | 41.1% | (many diffs) | |
| 22 | human | 79608 | RIC3 | RIC3 acetylcholine receptor... | XR_002957193.1 | 36.2% | (many diffs) | |
| 23 | human | 79608 | RIC3 | RIC3 acetylcholine receptor... | XR_930897.3 | 35.2% | (many diffs) | |
| 24 | human | 79608 | RIC3 | RIC3 acetylcholine receptor... | XR_930898.3 | 35% | (many diffs) | |
| 25 | human | 79608 | RIC3 | RIC3 acetylcholine receptor... | XR_002957192.1 | 28.9% | (many diffs) | |
| 26 | human | 79608 | RIC3 | RIC3 acetylcholine receptor... | XR_930901.3 | 28.9% | (many diffs) | |
| 27 | human | 79608 | RIC3 | RIC3 acetylcholine receptor... | XR_930903.3 | 28.4% | (many diffs) | |
| 28 | human | 79608 | RIC3 | RIC3 acetylcholine receptor... | XR_428848.4 | 28.3% | (many diffs) | |
| 29 | human | 79608 | RIC3 | RIC3 acetylcholine receptor... | XR_002957194.1 | 27.8% | (many diffs) | |
| 30 | human | 79608 | RIC3 | RIC3 acetylcholine receptor... | XR_002957195.1 | 25.1% | (many diffs) | |
| 31 | human | 79608 | RIC3 | RIC3 acetylcholine receptor... | XR_930904.3 | 19.8% | (many diffs) | |
| 32 | human | 79608 | RIC3 | RIC3 acetylcholine receptor... | XR_001747959.2 | 19.8% | (many diffs) | |
| 33 | human | 79608 | RIC3 | RIC3 acetylcholine receptor... | XR_930905.3 | 19.6% | (many diffs) | |
| 34 | human | 79608 | RIC3 | RIC3 acetylcholine receptor... | NR_144484.2 | 17.4% | (many diffs) | |
| 35 | human | 79608 | RIC3 | RIC3 acetylcholine receptor... | NR_144497.2 | 12.2% | (many diffs) | |
| 36 | mouse | 320360 | Ric3 | RIC3 acetylcholine receptor... | NM_001311161.1 | 87% | 63% | (many diffs) |
| 37 | mouse | 320360 | Ric3 | RIC3 acetylcholine receptor... | NM_001038624.1 | 86.7% | 62.8% | (many diffs) |
| 38 | mouse | 320360 | Ric3 | RIC3 acetylcholine receptor... | XM_017322302.1 | 54.8% | 79.3% | (many diffs) |
| 39 | mouse | 320360 | Ric3 | RIC3 acetylcholine receptor... | NM_178780.3 | 54.7% | 79% | (many diffs) |
| 40 | mouse | 320360 | Ric3 | RIC3 acetylcholine receptor... | XM_006507942.2 | 48.9% | 23.8% | (many diffs) |
| 41 | mouse | 320360 | Ric3 | RIC3 acetylcholine receptor... | XM_006507943.3 | 48.9% | 23.8% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 840
- ORF length:
- 771
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 caggcaccat ggcgtactcc acagtgcaga gagtcgctct ggcttctggg cttgtcctgg 121 ctctgtcgct gctgctgccc aaggccttcc tgtcccgcgg gaagcggcag gagccgccgc 181 cgacacctga aggaaaattg ggccgatttc cacctatgat gcatcatcac caggcacact 241 cagatggcca gactcctggg gctcgtttcc agaggtctca ccttgccgag gcatttgcaa 301 aggccaaagg atcaggtgga ggtgctggag gaggaggtag tggaagaggt ctgatggggc 361 agattattcc aatctacggt tttgggattt ttttatatat actgtacatt ctatttaagc 421 tctcaaaggg gaaaacaact gcagaggatg ggaaatgcta tactgccatg cctggaaaca 481 cccacaggaa aattaccagt tttgagcttg ctcaactgca agaaaaactg aaggagacag 541 aagcagccat ggaaaaatta ttcaacagag tgggacctaa tggtgagaga gcacagactg 601 tgacttctga ccaagagaaa cggttgctac atcagctccg agaaatcacc agggtcatga 661 aagaaggaaa attcattgac agattttctc cagagaaaga agctgaggag gccccttaca 721 tggaggactg ggaaggttac cctgaagaga cttacccaat ttatgacctt tcagactgta 781 tcaagcgtag gcaagaaaca atcttggtgg attacccTGA CCCAAAAGAA CTTTTCTGCT 841 GAAGAAATAG CTGAAAGAAT GGGAATGATA GAAGAGGAAG AATCAGATCA TTTGGGTTGG 901 GAAAGTCTGC CCACTGACCC CAGAGCCCAG GAAGATAATT CTGTTACCTC GTGTGATCCA 961 AAGCCAGAAA CATGTTCCTG CTGTTTTCAT GAAGACGAGG ATCCTGCTGT CTTGGCAGAG 1021 AATGCTGGAT TCAGTGCAGA TAGCTACCCT GAGCAAGAGG AAACCACCAA AGAAGAGTGG 1081 TCCCAAGACT TTAAAGATGA AGGGTTGGGC ATCAGCACCG ATAAAGCATA TACAGGCAGC 1141 ATGCTGAGGA AGCGTAACCC CCAGGGTTTA GAGTGAGACC CAGCTTTCTT GTACAAAGTG 1201 GTTGATATCG GTAAGCCTAT CCCTAACCCT CTCCTCGGTC TCGATTCTAC GTAGTAATGA 1261 ACTAGTCCGT AACTTGAAAG TATTTCGATT TCTTGGCTTT ATATATCTTG TGGAAAGGAC 1321 GATTCTCCCT GACTACGATA CCCGTCACGC GTTAAGTCga caatcaacct ctggattaca 1381 aaatttgtga aagatt