Construct: ORF TRCN0000488574
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF020586.1_s317c1
- DNA Barcode:
- GAGAACACTACTATTCATTTGGTT
- Epitope Tag:
- V5 (not translated due to prior stop codon)
- Notes:
- Has stop codon in insert
Originally Annotated References:
- Gene:
- MKNK1 (8569)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000488574
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 8569 | MKNK1 | MAPK interacting serine/thr... | XM_006711000.1 | 100% | 100% | |
| 2 | human | 8569 | MKNK1 | MAPK interacting serine/thr... | XM_006711001.3 | 98.3% | 97.4% | (many diffs) |
| 3 | human | 8569 | MKNK1 | MAPK interacting serine/thr... | XM_006711002.3 | 98.3% | 97.4% | (many diffs) |
| 4 | human | 8569 | MKNK1 | MAPK interacting serine/thr... | XM_006711003.1 | 98.3% | 97.4% | (many diffs) |
| 5 | human | 8569 | MKNK1 | MAPK interacting serine/thr... | NM_001135553.4 | 97.1% | 97.1% | 0_1ins36 |
| 6 | human | 8569 | MKNK1 | MAPK interacting serine/thr... | XM_024450508.1 | 97.1% | 97.1% | 0_1ins36 |
| 7 | human | 8569 | MKNK1 | MAPK interacting serine/thr... | XM_024450509.1 | 97.1% | 97.1% | 0_1ins36 |
| 8 | human | 8569 | MKNK1 | MAPK interacting serine/thr... | XM_024450510.1 | 97.1% | 97.1% | 0_1ins36 |
| 9 | human | 8569 | MKNK1 | MAPK interacting serine/thr... | NM_003684.7 | 88.6% | 88.6% | 0_1ins36;456_578del |
| 10 | human | 8569 | MKNK1 | MAPK interacting serine/thr... | XM_006711007.1 | 87% | 83% | 0_1ins67;69_70ins98 |
| 11 | human | 8569 | MKNK1 | MAPK interacting serine/thr... | XM_024450511.1 | 87% | 83% | 0_1ins67;69_70ins98 |
| 12 | human | 8569 | MKNK1 | MAPK interacting serine/thr... | XM_017002652.2 | 84.6% | 80.8% | (many diffs) |
| 13 | human | 8569 | MKNK1 | MAPK interacting serine/thr... | XM_017002653.1 | 84.6% | 80.8% | (many diffs) |
| 14 | human | 8569 | MKNK1 | MAPK interacting serine/thr... | XM_017002659.1 | 81.8% | 79.7% | 1004_1005ins44;1041_1042ins187 |
| 15 | human | 8569 | MKNK1 | MAPK interacting serine/thr... | XM_017002660.2 | 80.2% | 77.1% | (many diffs) |
| 16 | human | 8569 | MKNK1 | MAPK interacting serine/thr... | XM_017002661.2 | 80.2% | 77.1% | (many diffs) |
| 17 | human | 8569 | MKNK1 | MAPK interacting serine/thr... | XM_017002662.1 | 80.2% | 77.1% | (many diffs) |
| 18 | human | 8569 | MKNK1 | MAPK interacting serine/thr... | NM_198973.5 | 79% | 76.8% | 0_1ins36;968_969ins44;1005_1006ins187 |
| 19 | human | 8569 | MKNK1 | MAPK interacting serine/thr... | XM_024450512.1 | 79% | 76.8% | 0_1ins36;968_969ins44;1005_1006ins187 |
| 20 | human | 8569 | MKNK1 | MAPK interacting serine/thr... | XM_024450513.1 | 79% | 76.8% | 0_1ins36;968_969ins44;1005_1006ins187 |
| 21 | human | 8569 | MKNK1 | MAPK interacting serine/thr... | XM_024450514.1 | 79% | 76.8% | 0_1ins36;968_969ins44;1005_1006ins187 |
| 22 | human | 8569 | MKNK1 | MAPK interacting serine/thr... | XM_006711013.1 | 77.5% | 71.6% | 0_1ins211;103_104ins74 |
| 23 | human | 8569 | MKNK1 | MAPK interacting serine/thr... | XM_006711014.1 | 77.5% | 71.6% | 0_1ins211;103_104ins74 |
| 24 | human | 8569 | MKNK1 | MAPK interacting serine/thr... | XM_006711015.1 | 77.5% | 71.6% | 0_1ins211;103_104ins74 |
| 25 | human | 8569 | MKNK1 | MAPK interacting serine/thr... | NR_146512.2 | 46.3% | (many diffs) | |
| 26 | human | 8569 | MKNK1 | MAPK interacting serine/thr... | NR_024176.3 | 43.3% | (many diffs) | |
| 27 | human | 8569 | MKNK1 | MAPK interacting serine/thr... | NR_024174.3 | 43.3% | (many diffs) | |
| 28 | mouse | 17346 | Mknk1 | MAP kinase-interacting seri... | NM_001285487.1 | 85.7% | 90.3% | (many diffs) |
| 29 | mouse | 17346 | Mknk1 | MAP kinase-interacting seri... | XM_006502822.3 | 85.7% | 90.3% | (many diffs) |
| 30 | mouse | 17346 | Mknk1 | MAP kinase-interacting seri... | XM_006502823.3 | 85.3% | 90.6% | (many diffs) |
| 31 | mouse | 17346 | Mknk1 | MAP kinase-interacting seri... | NM_001285488.1 | 84.5% | 90.1% | (many diffs) |
| 32 | mouse | 17346 | Mknk1 | MAP kinase-interacting seri... | NM_021461.5 | 84.5% | 90.1% | (many diffs) |
| 33 | mouse | 17346 | Mknk1 | MAP kinase-interacting seri... | XM_006502825.3 | 71.6% | 72.8% | (many diffs) |
| 34 | mouse | 17346 | Mknk1 | MAP kinase-interacting seri... | XM_017320015.1 | 70.4% | 72.6% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 72
- ORF end:
- 1344
- ORF length:
- 1272
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 caggcttcac catggtatct tctcaaaagt tggaaaaacc tatagagatg ggcagtagcg 121 aaccccttcc catcgcagat ggtgacagga ggaggaagaa gaagcggagg ggccgggcca 181 ctgactcctt gccaggaaag tttgaagata tgtacaagct gacctctgaa ttgcttggag 241 agggagccta tgccaaagtt caaggtgccg tgagcctaca gaatggcaaa gagtatgccg 301 tcaaaatcat cgagaaacaa gcagggcaca gtcggagtag ggtgtttcga gaggtggaga 361 cgctgtatca gtgtcaggga aacaagaaca ttttggagct gattgagttc tttgaagatg 421 acacaaggtt ttacttggtc tttgagaaat tgcaaggagg ttccatctta gcccacatcc 481 agaagcaaaa gcacttcaat gagcgagaag ccagccgagt ggtgcgggac gttgctgctg 541 cccttgactt cctgcatacc aaaggcattg ctcatcgtga tctgaaacca gaaaatatat 601 tgtgtgaatc tccagaaaag gtgtctccag tgaaaatctg tgactttgac ttgggcagtg 661 ggatgaaact gaacaactcc tgtaccccca taaccacacc agagctgacc accccatgtg 721 gctctgcaga atacatggcc cctgaggtag tggaggtctt cacggaccag gccacattct 781 acgacaagcg ctgtgacctg tggagcctgg gcgtggtcct ctacatcatg ctgagtggct 841 acccaccctt cgtgggtcac tgcggggccg actgtggctg ggaccggggc gaggtctgca 901 gggtgtgcca gaacaagctg tttgaaagca tccaggaagg caagtatgag tttcctgaca 961 aggactgggc acacatctcc agtgaagcca aagacctcat ctccaagctc ctggtgcgag 1021 atgcaaagca gagacttagc gccgcccaag ttctgcagca cccatgGGTG CAGGGGCAAG 1081 CTCCAGAAAA GGGACTCCCC ACGCCGCAAG TCCTCCAGAG GAACAGCAGC ACAATGGACC 1141 TGACGCTCTT CGCAGCTGAG GCCATCGCCC TTAACCGCCA GCTATCTCAG CACGAAGAGA 1201 ACGAACTAGC AGAGGAGCCA GAGGCACTAG CTGATGGCCT CTGCTCCATG AAGCTTTCCC 1261 CTCCCTGCAA GTCACGCCTG GCCCGGAGAC GGGCCCTGGC CCAGGCAGGC CGTGGTGAAG 1321 ACAGGAGCCC GCCCACAGCA CTCTAGGACC CAGCTTTCTT GTACAAAGTG GTTGATATCG 1381 GTAAGCCTAT CCCTAACCCT CTCCTCGGTC TCGATTCTAC GTAGTAATGA ACTAGTCCGT 1441 AACTTGAAAG TATTTCGATT TCTTGGCTTT ATATATCTTG TGGAAAGGAC GAGAGAACAC 1501 TACTATTCAT TTGGTTACGC GTTAAGTCga caatcaacct ctggattaca aaatttgtga 1561 aagatt