Construct: ORF TRCN0000488574

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF020586.1_s317c1
DNA Barcode:
GAGAACACTACTATTCATTTGGTT
Epitope Tag:
V5 (not translated due to prior stop codon)
Notes:
Has stop codon in insert

Originally Annotated References:

Gene:
MKNK1 (8569)

Vector Information:

Vector Backbone:
pLX_317
Pol II Cassette 1:
SV40-PuroR
Pol II Cassette 2:
EF1a-TRCN0000488574
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
n/a

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 8569 MKNK1 MAPK interacting serine/thr... XM_006711000.1 100% 100%
2 human 8569 MKNK1 MAPK interacting serine/thr... XM_006711001.3 98.3% 97.4% (many diffs)
3 human 8569 MKNK1 MAPK interacting serine/thr... XM_006711002.3 98.3% 97.4% (many diffs)
4 human 8569 MKNK1 MAPK interacting serine/thr... XM_006711003.1 98.3% 97.4% (many diffs)
5 human 8569 MKNK1 MAPK interacting serine/thr... NM_001135553.4 97.1% 97.1% 0_1ins36
6 human 8569 MKNK1 MAPK interacting serine/thr... XM_024450508.1 97.1% 97.1% 0_1ins36
7 human 8569 MKNK1 MAPK interacting serine/thr... XM_024450509.1 97.1% 97.1% 0_1ins36
8 human 8569 MKNK1 MAPK interacting serine/thr... XM_024450510.1 97.1% 97.1% 0_1ins36
9 human 8569 MKNK1 MAPK interacting serine/thr... NM_003684.7 88.6% 88.6% 0_1ins36;456_578del
10 human 8569 MKNK1 MAPK interacting serine/thr... XM_006711007.1 87% 83% 0_1ins67;69_70ins98
11 human 8569 MKNK1 MAPK interacting serine/thr... XM_024450511.1 87% 83% 0_1ins67;69_70ins98
12 human 8569 MKNK1 MAPK interacting serine/thr... XM_017002652.2 84.6% 80.8% (many diffs)
13 human 8569 MKNK1 MAPK interacting serine/thr... XM_017002653.1 84.6% 80.8% (many diffs)
14 human 8569 MKNK1 MAPK interacting serine/thr... XM_017002659.1 81.8% 79.7% 1004_1005ins44;1041_1042ins187
15 human 8569 MKNK1 MAPK interacting serine/thr... XM_017002660.2 80.2% 77.1% (many diffs)
16 human 8569 MKNK1 MAPK interacting serine/thr... XM_017002661.2 80.2% 77.1% (many diffs)
17 human 8569 MKNK1 MAPK interacting serine/thr... XM_017002662.1 80.2% 77.1% (many diffs)
18 human 8569 MKNK1 MAPK interacting serine/thr... NM_198973.5 79% 76.8% 0_1ins36;968_969ins44;1005_1006ins187
19 human 8569 MKNK1 MAPK interacting serine/thr... XM_024450512.1 79% 76.8% 0_1ins36;968_969ins44;1005_1006ins187
20 human 8569 MKNK1 MAPK interacting serine/thr... XM_024450513.1 79% 76.8% 0_1ins36;968_969ins44;1005_1006ins187
21 human 8569 MKNK1 MAPK interacting serine/thr... XM_024450514.1 79% 76.8% 0_1ins36;968_969ins44;1005_1006ins187
22 human 8569 MKNK1 MAPK interacting serine/thr... XM_006711013.1 77.5% 71.6% 0_1ins211;103_104ins74
23 human 8569 MKNK1 MAPK interacting serine/thr... XM_006711014.1 77.5% 71.6% 0_1ins211;103_104ins74
24 human 8569 MKNK1 MAPK interacting serine/thr... XM_006711015.1 77.5% 71.6% 0_1ins211;103_104ins74
25 human 8569 MKNK1 MAPK interacting serine/thr... NR_146512.2 46.3% (many diffs)
26 human 8569 MKNK1 MAPK interacting serine/thr... NR_024176.3 43.3% (many diffs)
27 human 8569 MKNK1 MAPK interacting serine/thr... NR_024174.3 43.3% (many diffs)
28 mouse 17346 Mknk1 MAP kinase-interacting seri... NM_001285487.1 85.7% 90.3% (many diffs)
29 mouse 17346 Mknk1 MAP kinase-interacting seri... XM_006502822.3 85.7% 90.3% (many diffs)
30 mouse 17346 Mknk1 MAP kinase-interacting seri... XM_006502823.3 85.3% 90.6% (many diffs)
31 mouse 17346 Mknk1 MAP kinase-interacting seri... NM_001285488.1 84.5% 90.1% (many diffs)
32 mouse 17346 Mknk1 MAP kinase-interacting seri... NM_021461.5 84.5% 90.1% (many diffs)
33 mouse 17346 Mknk1 MAP kinase-interacting seri... XM_006502825.3 71.6% 72.8% (many diffs)
34 mouse 17346 Mknk1 MAP kinase-interacting seri... XM_017320015.1 70.4% 72.6% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
72
ORF end:
1344
ORF length:
1272
Sequence:
1tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag
61caggcttcac catggtatct tctcaaaagt tggaaaaacc tatagagatg ggcagtagcg
121aaccccttcc catcgcagat ggtgacagga ggaggaagaa gaagcggagg ggccgggcca
181ctgactcctt gccaggaaag tttgaagata tgtacaagct gacctctgaa ttgcttggag
241agggagccta tgccaaagtt caaggtgccg tgagcctaca gaatggcaaa gagtatgccg
301tcaaaatcat cgagaaacaa gcagggcaca gtcggagtag ggtgtttcga gaggtggaga
361cgctgtatca gtgtcaggga aacaagaaca ttttggagct gattgagttc tttgaagatg
421acacaaggtt ttacttggtc tttgagaaat tgcaaggagg ttccatctta gcccacatcc
481agaagcaaaa gcacttcaat gagcgagaag ccagccgagt ggtgcgggac gttgctgctg
541cccttgactt cctgcatacc aaaggcattg ctcatcgtga tctgaaacca gaaaatatat
601tgtgtgaatc tccagaaaag gtgtctccag tgaaaatctg tgactttgac ttgggcagtg
661ggatgaaact gaacaactcc tgtaccccca taaccacacc agagctgacc accccatgtg
721gctctgcaga atacatggcc cctgaggtag tggaggtctt cacggaccag gccacattct
781acgacaagcg ctgtgacctg tggagcctgg gcgtggtcct ctacatcatg ctgagtggct
841acccaccctt cgtgggtcac tgcggggccg actgtggctg ggaccggggc gaggtctgca
901gggtgtgcca gaacaagctg tttgaaagca tccaggaagg caagtatgag tttcctgaca
961aggactgggc acacatctcc agtgaagcca aagacctcat ctccaagctc ctggtgcgag
1021atgcaaagca gagacttagc gccgcccaag ttctgcagca cccatgGGTG CAGGGGCAAG
1081CTCCAGAAAA GGGACTCCCC ACGCCGCAAG TCCTCCAGAG GAACAGCAGC ACAATGGACC
1141TGACGCTCTT CGCAGCTGAG GCCATCGCCC TTAACCGCCA GCTATCTCAG CACGAAGAGA
1201ACGAACTAGC AGAGGAGCCA GAGGCACTAG CTGATGGCCT CTGCTCCATG AAGCTTTCCC
1261CTCCCTGCAA GTCACGCCTG GCCCGGAGAC GGGCCCTGGC CCAGGCAGGC CGTGGTGAAG
1321ACAGGAGCCC GCCCACAGCA CTCTAGGACC CAGCTTTCTT GTACAAAGTG GTTGATATCG
1381GTAAGCCTAT CCCTAACCCT CTCCTCGGTC TCGATTCTAC GTAGTAATGA ACTAGTCCGT
1441AACTTGAAAG TATTTCGATT TCTTGGCTTT ATATATCTTG TGGAAAGGAC GAGAGAACAC
1501TACTATTCAT TTGGTTACGC GTTAAGTCga caatcaacct ctggattaca aaatttgtga
1561aagatt

Download FASTA (ORF) (Full)

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