Construct: ORF ccsbBroad304_00472

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF005912.1_s304c1, BRDN0000407719
Derived from:
ccsbBroadEn_00472
DNA Barcode:
None
Epitope Tag:
V5 (not translated due to prior stop codon)
Notes:
Early stop codon detected

Originally Annotated References:

Gene:
EBF1 (1879)

Vector Information:

Vector Backbone:
pLX_304
Pol II Cassette 1:
PGK-BlastR
Pol II Cassette 2:
CMV-ccsbBroad304_00472
Selection Marker:
BlastR
Visible Reporter:
n/a
Epitope Tag:
n/a

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 1879 EBF1 EBF transcription factor 1 NM_024007.5 99.9% 62.9% 1116delT
2 human 1879 EBF1 EBF transcription factor 1 NM_001290360.3 99.7% 62.8% 778_780delGCA;1119delT
3 human 1879 EBF1 EBF transcription factor 1 NM_001324107.2 98.7% 61.5% 754_755insGTACGCCCTCTTATCTGGAA;756_757insT;1095delT
4 human 1879 EBF1 EBF transcription factor 1 NM_001324108.2 98.5% 61.5% 753_754ins24;1092delT
5 human 1879 EBF1 EBF transcription factor 1 NM_001324103.2 96.9% 61.6% (many diffs)
6 human 1879 EBF1 EBF transcription factor 1 XM_017009193.1 96.9% 61.6% (many diffs)
7 human 1879 EBF1 EBF transcription factor 1 NM_001324101.2 96.8% 61.5% (many diffs)
8 human 1879 EBF1 EBF transcription factor 1 XM_017009192.1 96.8% 61.5% (many diffs)
9 human 1879 EBF1 EBF transcription factor 1 XM_017009194.1 95.8% 60.3% (many diffs)
10 human 1879 EBF1 EBF transcription factor 1 NM_001324106.2 95.6% 60.3% (many diffs)
11 human 1879 EBF1 EBF transcription factor 1 XM_017009195.1 95.6% 60.3% (many diffs)
12 human 1879 EBF1 EBF transcription factor 1 NM_182708.3 94.6% 57.6% 482_483ins69;684_685ins24;1023delT
13 human 1879 EBF1 EBF transcription factor 1 NM_001364156.1 93.4% 55.4% (many diffs)
14 human 1879 EBF1 EBF transcription factor 1 XM_017009197.1 90.5% 54.3% (many diffs)
15 human 1879 EBF1 EBF transcription factor 1 NM_001364157.1 90.4% 54.3% (many diffs)
16 human 1879 EBF1 EBF transcription factor 1 XM_017009196.1 90.4% 54.3% (many diffs)
17 human 1879 EBF1 EBF transcription factor 1 NM_001364155.1 89.7% 70% 1116delT;1369_1370ins180
18 human 1879 EBF1 EBF transcription factor 1 NM_001324109.2 86.9% 68.3% (many diffs)
19 human 1879 EBF1 EBF transcription factor 1 NM_001364159.1 77.4% 40.4% 0_1ins399;717delT
20 human 1879 EBF1 EBF transcription factor 1 NM_001324111.2 77.3% 40.3% 0_1ins399;379_381delGCA;720delT
21 human 1879 EBF1 EBF transcription factor 1 XM_024454393.1 77.3% 40.3% 0_1ins399;379_381delGCA;720delT
22 human 1879 EBF1 EBF transcription factor 1 NM_001364158.1 76% 39% 0_1ins399;354_355ins24;693delT
23 human 1879 EBF1 EBF transcription factor 1 XM_024454390.1 74.8% 39.5% (many diffs)
24 human 1879 EBF1 EBF transcription factor 1 XM_024454391.1 74.8% 39.5% (many diffs)
25 human 1879 EBF1 EBF transcription factor 1 XM_024454392.1 73.6% 38.3% (many diffs)
26 human 253738 EBF3 EBF transcription factor 3 NM_001005463.3 72.7% 61.7% (many diffs)
27 human 1879 EBF1 EBF transcription factor 1 XM_017009201.2 64.9% 27.9% 0_1ins621;495delT
28 human 1879 EBF1 EBF transcription factor 1 XM_017009199.1 62.6% 27.3% (many diffs)
29 human 1879 EBF1 EBF transcription factor 1 XM_017009200.2 62.6% 27.3% (many diffs)
30 human 1879 EBF1 EBF transcription factor 1 XM_017009203.1 60.2% 92.8% (many diffs)
31 human 1879 EBF1 EBF transcription factor 1 XM_017009202.1 60.1% 92.6% (many diffs)
32 human 1879 EBF1 EBF transcription factor 1 XM_017009204.1 52.2% 12.6% (many diffs)
33 mouse 13591 Ebf1 early B cell factor 1 NM_007897.3 94% 62.9% (many diffs)
34 mouse 13591 Ebf1 early B cell factor 1 NM_001290709.1 93.9% 62.8% (many diffs)
35 mouse 13591 Ebf1 early B cell factor 1 XM_006532155.3 92.8% 61.5% (many diffs)
36 mouse 13591 Ebf1 early B cell factor 1 XM_006532156.3 92.7% 61.5% (many diffs)
37 mouse 13591 Ebf1 early B cell factor 1 XM_006532157.3 87.4% 55.9% (many diffs)
38 mouse 13591 Ebf1 early B cell factor 1 NM_001290710.1 83.4% 68.5% (many diffs)
39 mouse 13591 Ebf1 early B cell factor 1 NM_001290711.1 72.5% 40.4% (many diffs)
40 mouse 13591 Ebf1 early B cell factor 1 XM_006532158.3 72.4% 40.3% (many diffs)
41 mouse 13591 Ebf1 early B cell factor 1 XM_017314250.1 72.4% 40.3% (many diffs)
42 mouse 13591 Ebf1 early B cell factor 1 XM_017314251.1 71.2% 39% (many diffs)
43 mouse 13591 Ebf1 early B cell factor 1 XM_017314252.1 71.2% 39% (many diffs)
44 mouse 13593 Ebf3 early B cell factor 3 XM_011241672.1 71% 64.8% (many diffs)
45 mouse 13593 Ebf3 early B cell factor 3 XM_006507334.1 68.2% 62.1% (many diffs)
46 mouse 13593 Ebf3 early B cell factor 3 XM_006507337.3 63.8% 73.6% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
66
ORF end:
1194
ORF length:
1128
Sequence:
1ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag
61ttggcatgtt tgggattcag gaaagcatcc aacggagtgg aagcagcatg aaggaagagc
121cgctgggcag cggcatgaac gcggtgcgga cgtggatgca gggcgccggg gtgctggacg
181ccaacacggc ggcgcagagc ggggtgggtc tggcccgggc tcactttgag aagcagccgc
241cttccaatct gcggaaatcc aacttcttcc acttcgtcct ggccctctac gacagacagg
301gccagcccgt ggagatcgag aggacagcgt ttgtggggtt cgtggagaag gaaaaagaag
361ccaacagcga aaagaccaat aacggaattc actaccggct tcagcttctc tacagcaatg
421ggataaggac ggagcaggat ttctacgtgc gcctcattga ctccatgaca aaacaagcca
481tagtgtatga aggccaagac aagaacccag aaatgtgccg agtcttgctc acacatgaga
541tcatgtgcag ccgctgttgt gacaagaaaa gctgtggcaa ccgaaatgag actccctcag
601atccagtgat aattgacagg ttcttcttga aatttttcct caaatgtaac caaaattgcc
661taaagaatgc gggaaaccca cgtgacatgc ggagattcca ggtcgtggtg tctacgacag
721tcaatgtgga tggccatgtc ctggcagtct ctgataacat gtttgtccat aataattcca
781agcatgggcg gagggctcgg aggcttgacc cctcggaagg tacgccctct tatctggaac
841atgctactcc ctgtatcaaa gccatcagcc cgagtgaagg atggacgacg ggaggtgcga
901ctgtgatcat cataggggac aatttctttg atgggttaca ggtcatattc ggtaccatgc
961tggtctggag tgagttgatc actcctcatg ccatccgtgt gcagacccct cctcggcaca
1021tccctggtgt tgtggaagtc acactgtcct acaaatctaa gcagttctgc aaaggaacac
1081caggcagatt catttataca gcgctcaacg aacccaccat cgattatggt ttccagaggt
1141tacagaaggt cattcctcgg caccctggtg accctGAGCG TTGCCAAAGG AAGTAATACT
1201CAAAAGGGCT GCGGATCTGG TAGAAGCACT GTATGGGATG CCACACAACA ACCAGGAAAT
1261CATTCTGAAG AGAGCGGCCG ACATTGCCGA GGCCCTGTAC AGTGTTCCCC GCAACCACAA
1321CCAACTCCCG GCCCTTGCTA ACACCTCGGT CCACGCAGGG ATGATGGGCG TGAATTCGTT
1381CAGTGGACAA CTGGCCGTGA ATGTCTCCGA GGCATCACAA GCCACCAATC AGGGTTTCAC
1441CCGCAACTCA AGCAGCGTAT CACCACACGG GTACGTGCCG AGCACCACTC CCCAGCAGAC
1501CAACTATAAC TCCGTCACCA CGAGCATGAA CGGATACGGC TCTGCCGCAA TGTCCAATTT
1561GGGCGGCTCC CCCACCTTCC TCAACGGCTC AGCTGCCAAC TCCCCCTATG CCATAGTGCC
1621ATCCAGCCCC ACCATGGCCT CCTCCACAAG CCTCCCCTCC AACTGCAGCA GCTCCTCGGG
1681CATCTTCTCC TTCTCACCAG CCAACATGGT CTCAGCCGTG AAACAGAAGA GTGCTTTCGC
1741ACCAGTCGTC AGACCCCAGA CCTCCCCACC TCCCACCTGC ACCAGCACCA ACGGGAACAG
1801CCTGCAAGCG ATATCTGGCA TGATTGTTCC TCCTATGTGC CCAACTTTCT TGTACaaagt
1861ggttggtaag cctatcccta accctctcct cggtctcgat tctacgtagt aatgagctag
1921cgctaaccgg tggcgcgtta agtcgacaat caacctctgg atta

Download FASTA (ORF) (Full)