Construct: ORF TRCN0000468031

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF002810.2_s317c1
Derived from:
ccsbBroadEn_14563
DNA Barcode:
AGCATTTCATGGTGGACCTTTAGT
Epitope Tag:
V5 (not translated due to frame shift)
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
CAMK2B (816)

Vector Information:

Vector Backbone:
pLX_317
Pol II Cassette 1:
SV40-PuroR
Pol II Cassette 2:
EF1a-TRCN0000468031
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 816 CAMK2B calcium/calmodulin dependen... NM_172081.3 96% 96% (many diffs)
2 human 816 CAMK2B calcium/calmodulin dependen... XM_017012664.1 95.8% 95.8% (many diffs)
3 human 816 CAMK2B calcium/calmodulin dependen... NM_172079.2 93.2% 93.2% (many diffs)
4 human 816 CAMK2B calcium/calmodulin dependen... NM_172080.3 93% 93% (many diffs)
5 human 816 CAMK2B calcium/calmodulin dependen... XM_005249864.3 92.7% 91.8% (many diffs)
6 human 816 CAMK2B calcium/calmodulin dependen... XM_017012665.1 92.5% 91.6% (many diffs)
7 human 816 CAMK2B calcium/calmodulin dependen... XM_005249862.3 91.5% 91.4% (many diffs)
8 human 816 CAMK2B calcium/calmodulin dependen... NM_172083.3 91.2% 91.2% (many diffs)
9 human 816 CAMK2B calcium/calmodulin dependen... NM_001293170.2 89% 88.9% (many diffs)
10 human 816 CAMK2B calcium/calmodulin dependen... NM_172078.3 89% 88.9% (many diffs)
11 human 816 CAMK2B calcium/calmodulin dependen... XM_006715784.2 88.4% 87.5% (many diffs)
12 human 816 CAMK2B calcium/calmodulin dependen... NM_172084.3 85.2% 85.2% (many diffs)
13 human 816 CAMK2B calcium/calmodulin dependen... NM_172082.3 83.5% 82.5% (many diffs)
14 human 816 CAMK2B calcium/calmodulin dependen... XM_017012662.1 79.8% 79.8% (many diffs)
15 human 816 CAMK2B calcium/calmodulin dependen... XM_006715781.2 76.8% 76.8% (many diffs)
16 human 816 CAMK2B calcium/calmodulin dependen... XM_024446945.1 76.8% 76.7% (many diffs)
17 human 818 CAMK2G calcium/calmodulin dependen... NM_172173.3 76.5% 84.4% (many diffs)
18 human 818 CAMK2G calcium/calmodulin dependen... NM_001367530.1 75.8% 83.6% (many diffs)
19 human 818 CAMK2G calcium/calmodulin dependen... NM_001222.4 75.2% 84.5% (many diffs)
20 human 818 CAMK2G calcium/calmodulin dependen... XM_017016730.1 75.1% 82.2% (many diffs)
21 human 818 CAMK2G calcium/calmodulin dependen... NM_001367517.1 75% 82.8% (many diffs)
22 human 818 CAMK2G calcium/calmodulin dependen... NM_172170.5 75% 82.8% (many diffs)
23 human 818 CAMK2G calcium/calmodulin dependen... NM_001367516.1 74.7% 82.3% (many diffs)
24 human 818 CAMK2G calcium/calmodulin dependen... NM_001367532.1 73.7% 81.3% (many diffs)
25 human 818 CAMK2G calcium/calmodulin dependen... NM_001367531.1 73.7% 80.7% (many diffs)
26 human 816 CAMK2B calcium/calmodulin dependen... XM_017012661.1 73.5% 73.4% (many diffs)
27 human 818 CAMK2G calcium/calmodulin dependen... NM_001367545.1 72.9% 78.8% (many diffs)
28 human 818 CAMK2G calcium/calmodulin dependen... NM_172169.3 72.8% 80.3% (many diffs)
29 human 818 CAMK2G calcium/calmodulin dependen... NM_001367536.1 72.7% 79.2% (many diffs)
30 human 816 CAMK2B calcium/calmodulin dependen... NM_001220.5 72.4% 72.3% (many diffs)
31 human 816 CAMK2B calcium/calmodulin dependen... XM_011515555.1 72.4% 72.3% (many diffs)
32 human 818 CAMK2G calcium/calmodulin dependen... XM_005270205.1 72.2% 81.1% (many diffs)
33 human 818 CAMK2G calcium/calmodulin dependen... XM_006717998.1 72.2% 79% (many diffs)
34 human 816 CAMK2B calcium/calmodulin dependen... XM_011515556.1 72.1% 69% (many diffs)
35 human 818 CAMK2G calcium/calmodulin dependen... NM_001204492.1 72.1% 79.6% (many diffs)
36 human 816 CAMK2B calcium/calmodulin dependen... XM_017012660.1 72% 72% (many diffs)
37 human 816 CAMK2B calcium/calmodulin dependen... XM_011515557.1 72% 68.8% (many diffs)
38 human 816 CAMK2B calcium/calmodulin dependen... XM_011515554.1 71.9% 71.9% (many diffs)
39 human 816 CAMK2B calcium/calmodulin dependen... XM_011515553.1 71.9% 71.8% (many diffs)
40 human 818 CAMK2G calcium/calmodulin dependen... NM_001367518.1 71.6% 78% (many diffs)
41 human 818 CAMK2G calcium/calmodulin dependen... XM_024448218.1 71.6% 78.8% (many diffs)
42 human 818 CAMK2G calcium/calmodulin dependen... NM_001367533.1 71.4% 78.7% (many diffs)
43 human 818 CAMK2G calcium/calmodulin dependen... NM_001367543.1 70.9% 77.6% (many diffs)
44 human 816 CAMK2B calcium/calmodulin dependen... XM_011515558.2 70.8% 63.2% (many diffs)
45 human 816 CAMK2B calcium/calmodulin dependen... XM_011515550.1 70.5% 70.5% (many diffs)
46 human 816 CAMK2B calcium/calmodulin dependen... XM_017012670.2 70.3% 70.3% (many diffs)
47 human 816 CAMK2B calcium/calmodulin dependen... XM_011515552.1 70.3% 70.3% (many diffs)
48 human 818 CAMK2G calcium/calmodulin dependen... NM_001367527.1 70.2% 75.8% (many diffs)
49 human 818 CAMK2G calcium/calmodulin dependen... NM_172171.2 70.1% 77.3% (many diffs)
50 human 816 CAMK2B calcium/calmodulin dependen... XM_011515551.1 69.6% 66.4% (many diffs)
51 human 816 CAMK2B calcium/calmodulin dependen... XM_011515549.1 69.5% 69.4% (many diffs)
52 human 818 CAMK2G calcium/calmodulin dependen... NM_001367547.1 69.2% 75.9% (many diffs)
53 human 818 CAMK2G calcium/calmodulin dependen... NM_001367546.1 69% 75.1% (many diffs)
54 human 818 CAMK2G calcium/calmodulin dependen... XM_006717996.1 69% 75.5% (many diffs)
55 human 818 CAMK2G calcium/calmodulin dependen... NM_001367525.1 68.9% 75.9% (many diffs)
56 human 818 CAMK2G calcium/calmodulin dependen... XM_005270198.1 68.7% 75.8% (many diffs)
57 human 816 CAMK2B calcium/calmodulin dependen... XM_017012668.2 68.3% 68.3% (many diffs)
58 human 816 CAMK2B calcium/calmodulin dependen... XM_024446946.1 68.1% 68.1% (many diffs)
59 human 816 CAMK2B calcium/calmodulin dependen... XM_011515547.1 68% 67.9% (many diffs)
60 human 818 CAMK2G calcium/calmodulin dependen... NM_001367548.1 67.6% 74% (many diffs)
61 human 818 CAMK2G calcium/calmodulin dependen... NM_001367544.1 67.6% 74.5% (many diffs)
62 human 816 CAMK2B calcium/calmodulin dependen... XM_017012667.2 67.1% 66.9% (many diffs)
63 human 818 CAMK2G calcium/calmodulin dependen... NM_001367534.1 66.5% 72.8% (many diffs)
64 human 816 CAMK2B calcium/calmodulin dependen... XM_017012666.2 65.2% 65.1% (many diffs)
65 human 816 CAMK2B calcium/calmodulin dependen... XM_017012669.2 64.1% 63% (many diffs)
66 human 818 CAMK2G calcium/calmodulin dependen... XM_024448219.1 62.6% 68% (many diffs)
67 human 818 CAMK2G calcium/calmodulin dependen... NM_001367539.1 58.5% 63.5% (many diffs)
68 human 818 CAMK2G calcium/calmodulin dependen... NM_001367537.1 57.6% 59.8% (many diffs)
69 human 816 CAMK2B calcium/calmodulin dependen... XM_017012663.2 50.8% 47.9% (many diffs)
70 human 816 CAMK2B calcium/calmodulin dependen... XM_011515559.2 49.8% 49.7% (many diffs)
71 mouse 12323 Camk2b calcium/calmodulin-dependen... XM_006514479.2 88.4% 95.2% (many diffs)
72 mouse 12323 Camk2b calcium/calmodulin-dependen... XM_006514476.1 85.9% 92.4% (many diffs)
73 mouse 12323 Camk2b calcium/calmodulin-dependen... XM_006514477.1 85.7% 92.2% (many diffs)
74 mouse 12323 Camk2b calcium/calmodulin-dependen... XM_006514475.1 84.3% 90.7% (many diffs)
75 mouse 12323 Camk2b calcium/calmodulin-dependen... XM_017314240.1 84.2% 90.7% (many diffs)
76 mouse 12323 Camk2b calcium/calmodulin-dependen... NM_001174054.1 84.1% 90.5% (many diffs)
77 mouse 12323 Camk2b calcium/calmodulin-dependen... NM_007595.5 82.1% 88.1% (many diffs)
78 mouse 12323 Camk2b calcium/calmodulin-dependen... XM_017314239.1 82% 88.2% (many diffs)
79 mouse 12323 Camk2b calcium/calmodulin-dependen... NM_001174053.1 81.3% 87.5% (many diffs)
80 mouse 12325 Camk2g calcium/calmodulin-dependen... NM_001039139.2 73.7% 84.5% (many diffs)
81 mouse 12325 Camk2g calcium/calmodulin-dependen... NM_001039138.2 73.6% 82.8% (many diffs)
82 mouse 12325 Camk2g calcium/calmodulin-dependen... XM_017315827.1 73% 83.6% (many diffs)
83 mouse 12325 Camk2g calcium/calmodulin-dependen... NM_178597.5 72.3% 80.7% (many diffs)
84 mouse 12323 Camk2b calcium/calmodulin-dependen... XM_006514469.2 70.9% 76.3% (many diffs)
85 mouse 12325 Camk2g calcium/calmodulin-dependen... XM_006518496.3 70.8% 79.6% (many diffs)
86 mouse 12323 Camk2b calcium/calmodulin-dependen... XM_006514470.3 70.8% 76.2% (many diffs)
87 mouse 12325 Camk2g calcium/calmodulin-dependen... XM_017315826.1 70.8% 81.1% (many diffs)
88 mouse 12325 Camk2g calcium/calmodulin-dependen... XM_006518498.2 70.2% 80.3% (many diffs)
89 mouse 12325 Camk2g calcium/calmodulin-dependen... XM_006518494.2 69.6% 77.6% (many diffs)
90 mouse 12323 Camk2b calcium/calmodulin-dependen... XM_006514467.2 69.3% 74.6% (many diffs)
91 mouse 12323 Camk2b calcium/calmodulin-dependen... XM_017314238.1 69.2% 74.4% (many diffs)
92 mouse 12325 Camk2g calcium/calmodulin-dependen... XM_006518492.2 69% 77.3% (many diffs)
93 mouse 12323 Camk2b calcium/calmodulin-dependen... XM_017314237.1 68.2% 73.4% (many diffs)
94 mouse 12325 Camk2g calcium/calmodulin-dependen... XM_006518490.3 67.9% 75.5% (many diffs)
95 mouse 12323 Camk2b calcium/calmodulin-dependen... XM_006514465.2 66.8% 71.7% (many diffs)
96 mouse 12323 Camk2b calcium/calmodulin-dependen... XM_006514464.2 66.7% 71.8% (many diffs)
97 mouse 12325 Camk2g calcium/calmodulin-dependen... XM_006518489.2 66.5% 74.5% (many diffs)
98 mouse 12325 Camk2g calcium/calmodulin-dependen... XM_006518488.2 65.5% 72.8% (many diffs)
99 mouse 12325 Camk2g calcium/calmodulin-dependen... XM_006518500.3 54.8% 59.5% (many diffs)
100 mouse 108058 Camk2d calcium/calmodulin-dependen... XM_006500836.3 50.2% 56.2% (many diffs)
101 mouse 12323 Camk2b calcium/calmodulin-dependen... XM_017314241.1 43.4% 42.4% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
66
ORF end:
1518
ORF length:
1452
Sequence:
1tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag
61ttggcatggc caccacggtg acctgcaccc gcttcaccga cgagtaccag ctctacgagg
121atattggcaa gggggctttc tctgtggtcc gacgctgtgt caagctctgc accggccatg
181agtatgcagc caagatcatc aacaccaaga agctgtcagc cagagatcac cagaagctgg
241agagagaggc tcggatctgc cgccttctga agcattccaa catcgtgcgt ctccacgaca
301gcatctccga ggagggcttc cactacctgg tcttcgatct ggtcactggt ggggagctct
361ttgaagacat tgtggcgaga gagtactaca gcgaggctga tgccagtcac tgtatccagc
421agatcctgga ggccgttctc cattgtcacc aaatgggggt cgtccacaga gacctcaagc
481cggagaacct gcttctggcc agcaagtgca aaggggctgc agtgaagctg gcagacttcg
541gcctagctat cgaggtgcag ggggaccagc aggcatggtt tggtttcgct ggcacaccag
601gctacctgtc ccctgaggtc cttcgcaaag aggcgtatgg caagcctgtg gacatctggg
661catgtggggt gatcctgtac atcctgctcg tgggctaccc acccttctgg gacgaggacc
721agcacaagct gtaccagcag atcaaggctg gtgcctatga cttcccgtcc cctgagtggg
781acaccgtcac tcctgaagcc aaaaacctca tcaaccagat gctgaccatc aaccctgcca
841agcgcatcac agcccatgag gccctgaagc acccgtgggt ctgccaacgc tccacggtag
901catccatgat gcacagacag gagactgtgg agtgtctgaa aaagttcaat gccaggagaa
961agctcaaggg agccatcctc accaccatgc tggccacacg gaatttctca gcagccaaga
1021gtttactcaa caagaaagca gatggagtca agccccagac gaatagcacc aaaaacagtg
1081cagccgccac cagccccaaa gGGACGCTTC CTCCTGCCGC CCTGGAGTCT TCTGACAGTG
1141CCAATACCAC CATAGAGGAT GAAGACGCTA AAGCCCGGAA GCAGGAGATC ATTAAGACCA
1201CGGAGCAGCT CATCGAGGCC GTCAACAACG GTGACTTTGA GGCCTACGCG AAAATCTGTG
1261ACCCAGGGCT GACCTCGTTT GAGCCTGAAG CACTGGGCAA CCTGGTTGAA GGGATGGACT
1321TCCACAGATT CTACTTCGAG AACCTGCTGG CCAAGAACAG CAAGCCGATC CACACGACCA
1381TCCTGAACCC ACACGTGCAC GTCATTGGAG AGGATGCCGC CTGCATCGCT TACATCCGGC
1441TCACGCAGTA CATTGACGGG CAGGGCCGGC CCCGCACCAG CCAGTCTGAG GAGACCCGCG
1501TGTGGCCCCG CTGCAGTGCC CAACTTTCTT GTACAAAGTG GTTGATATCG GTAAGCCTAT
1561CCCTAACCCT CTCCTCGGTC TCGATTCTAC GTAGTAATGA ACTAGTCCGT AACTTGAAAG
1621TATTTCGATT TCTTGGCTTT ATATATCTTG TGGAAAGGAC GAAGCATTTC ATGGTGGACC
1681TTTAGTACGC GTTAAGTCga caatcaacct ctggattaca aaatttgtga aagatt

Download FASTA (ORF) (Full)