Construct: ORF ccsbBroad304_07469
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF011988.1_s304c1, BRDN0000403790
- Derived from:
- ccsbBroadEn_07469
- DNA Barcode:
- None
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- PUM1 (9698)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_07469
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 9698 | PUM1 | pumilio RNA binding family ... | NM_014676.3 | 99.9% | 100% | 2337T>C |
| 2 | human | 9698 | PUM1 | pumilio RNA binding family ... | NM_001020658.2 | 99.8% | 99.8% | 2337T>C;2851_2856delGTAATT |
| 3 | mouse | 80912 | Pum1 | pumilio RNA-binding family ... | NM_001159603.1 | 93.9% | 98.9% | (many diffs) |
| 4 | mouse | 80912 | Pum1 | pumilio RNA-binding family ... | XM_017320459.1 | 93.8% | 98.9% | (many diffs) |
| 5 | mouse | 80912 | Pum1 | pumilio RNA-binding family ... | NM_001159604.1 | 93.7% | 98.8% | (many diffs) |
| 6 | mouse | 80912 | Pum1 | pumilio RNA-binding family ... | NM_030722.2 | 93.6% | 98.7% | (many diffs) |
| 7 | mouse | 80912 | Pum1 | pumilio RNA-binding family ... | XM_006539294.3 | 93.5% | 98.5% | (many diffs) |
| 8 | mouse | 80912 | Pum1 | pumilio RNA-binding family ... | XM_006539291.3 | 93.3% | 98.4% | (many diffs) |
| 9 | mouse | 80912 | Pum1 | pumilio RNA-binding family ... | XM_006539292.2 | 93.3% | 98.4% | (many diffs) |
| 10 | mouse | 80912 | Pum1 | pumilio RNA-binding family ... | XM_006539293.3 | 93.2% | 98.3% | (many diffs) |
| 11 | mouse | 80912 | Pum1 | pumilio RNA-binding family ... | XM_006539295.3 | 90.9% | 95.9% | (many diffs) |
| 12 | mouse | 80912 | Pum1 | pumilio RNA-binding family ... | XM_017320463.1 | 87.3% | 92.6% | (many diffs) |
| 13 | mouse | 80912 | Pum1 | pumilio RNA-binding family ... | XM_006539298.1 | 87.3% | 92.5% | (many diffs) |
| 14 | mouse | 80912 | Pum1 | pumilio RNA-binding family ... | XM_017320462.1 | 87.3% | 92.5% | (many diffs) |
| 15 | mouse | 80912 | Pum1 | pumilio RNA-binding family ... | XM_017320461.1 | 87.2% | 92.5% | (many diffs) |
| 16 | mouse | 80912 | Pum1 | pumilio RNA-binding family ... | XM_017320460.1 | 87.1% | 92.4% | (many diffs) |
| 17 | mouse | 80912 | Pum1 | pumilio RNA-binding family ... | XM_006539297.3 | 87% | 92.2% | (many diffs) |
| 18 | mouse | 80912 | Pum1 | pumilio RNA-binding family ... | XM_006539296.3 | 86.8% | 92.1% | (many diffs) |
| 19 | mouse | 80912 | Pum1 | pumilio RNA-binding family ... | XM_017320464.1 | 85.8% | 90.8% | (many diffs) |
| 20 | mouse | 80912 | Pum1 | pumilio RNA-binding family ... | NM_001159605.1 | 85.7% | 90.7% | (many diffs) |
| 21 | mouse | 80912 | Pum1 | pumilio RNA-binding family ... | XM_006539299.3 | 85.3% | 90.3% | (many diffs) |
| 22 | mouse | 80912 | Pum1 | pumilio RNA-binding family ... | XM_006539300.3 | 83.5% | 88.3% | (many diffs) |
| 23 | mouse | 80912 | Pum1 | pumilio RNA-binding family ... | XM_006539301.3 | 83.4% | 88.2% | (many diffs) |
| 24 | mouse | 80912 | Pum1 | pumilio RNA-binding family ... | XM_011250358.2 | 83.1% | 86.9% | (many diffs) |
| 25 | mouse | 80912 | Pum1 | pumilio RNA-binding family ... | XM_006539302.3 | 81.2% | 86.1% | (many diffs) |
| 26 | mouse | 80912 | Pum1 | pumilio RNA-binding family ... | XM_006539303.3 | 77.9% | 79.3% | (many diffs) |
| 27 | mouse | 80912 | Pum1 | pumilio RNA-binding family ... | XM_006539304.3 | 77.9% | 79.3% | (many diffs) |
| 28 | mouse | 80912 | Pum1 | pumilio RNA-binding family ... | XM_006539305.3 | 77.8% | 79% | (many diffs) |
| 29 | mouse | 80912 | Pum1 | pumilio RNA-binding family ... | XM_006539306.3 | 77.8% | 79% | (many diffs) |
| 30 | mouse | 80912 | Pum1 | pumilio RNA-binding family ... | XM_011250359.2 | 77.1% | 78.8% | (many diffs) |
| 31 | mouse | 80912 | Pum1 | pumilio RNA-binding family ... | XM_011250360.2 | 75.5% | 80.3% | (many diffs) |
| 32 | mouse | 80912 | Pum1 | pumilio RNA-binding family ... | NM_001159606.1 | 73.6% | 78.5% | (many diffs) |
| 33 | mouse | 80912 | Pum1 | pumilio RNA-binding family ... | XM_006539310.3 | 73.5% | 78.4% | (many diffs) |
| 34 | mouse | 80912 | Pum1 | pumilio RNA-binding family ... | XM_006539311.3 | 73.5% | 78.4% | (many diffs) |
| 35 | mouse | 80912 | Pum1 | pumilio RNA-binding family ... | XM_006539309.3 | 73.5% | 78.3% | (many diffs) |
| 36 | mouse | 80912 | Pum1 | pumilio RNA-binding family ... | XM_006539308.3 | 73.2% | 78.1% | (many diffs) |
| 37 | mouse | 80912 | Pum1 | pumilio RNA-binding family ... | XM_006539312.3 | 69.7% | 73.1% | (many diffs) |
| 38 | mouse | 80912 | Pum1 | pumilio RNA-binding family ... | XM_017320466.1 | 65.5% | 70% | (many diffs) |
| 39 | mouse | 80912 | Pum1 | pumilio RNA-binding family ... | XM_006539314.3 | 62.9% | 66.7% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 3624
- ORF length:
- 3558
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcatgag cgttgcatgt gtcttgaaga gaaaagcagt gctttggcag gactctttca 121 gcccccacct gaaacatcac cctcaagaac cagctaatcc caacatgcct gttgttttga 181 catctggaac agggtcgcaa gcgcagccac aaccagctgc aaatcaggct cttgcagctg 241 ggactcactc cagccctgtc ccaggatcta taggagttgc aggccgttcc caggacgacg 301 ctatggtgga ctacttcttt cagaggcagc atggtgagca gcttggggga ggaggaagtg 361 gaggaggcgg ctataataat agcaaacatc gatggcctac tggggataac attcatgcag 421 aacatcaggt gcgttccatg gatgaactga atcatgattt tcaagcactt gctctggagg 481 gaagagcgat gggagagcag ctcttgccag gtaaaaagtt ttgggaaaca gatgaatcca 541 gcaaagatgg accaaaagga atattcctgg gtgatcaatg gcgagacagt gcctggggaa 601 catcagatca ttcagtttcc cagccaatca tggtgcagag aagacctggt cagagtttcc 661 atgtgaacag tgaggtcaat tctgtactgt ccccacgatc ggagagtggg ggactaggcg 721 ttagcatggt ggagtatgtg ttgagctcat ccccgggcga ttcctgtcta agaaaaggag 781 gatttggccc aagggatgca gacagtgatg aaaacgacaa aggtgaaaag aagaacaagg 841 gtacgtttga tggagataag ctaggagatt tgaaggagga gggtgatgtg atggacaaga 901 ccaatggttt accagtgcag aatgggattg atgcagacgt caaagatttt agccgtaccc 961 ctggtaattg ccagaactct gctaatgaag tggatcttct gggtccaaac cagaatggtt 1021 ctgagggctt agcccagctg accagcacca atggtgccaa gcctgtggag gatttctcca 1081 acatggagtc ccagagtgtc cccttggacc ccatggaaca tgtgggcatg gagcctcttc 1141 agtttgatta ttcaggcacg caggtacctg tggactcagc agcagcaact gtgggacttt 1201 ttgactacaa ttctcaacaa cagctgttcc aaagacctaa tgcgcttgct gtccagcagt 1261 tgacagctgc tcagcagcag cagtatgcac tggcagctgc tcatcagccg cacatcggtt 1321 tagctcccgc tgcgtttgtc cccaatccat acatcatcag cgctgctccc ccagggacgg 1381 acccctacac agctggattg gctgcagcag cgacactagg cccagctgtg gtccctcacc 1441 agtattatgg agttactccc tggggagtct accctgccag tcttttccag cagcaagctg 1501 ccgctgccgc tgcagcaact aattcagcta atcaacagac caccccacag gctcagcaag 1561 gacagcagca ggttctccgt ggaggagcca gccaacgtcc tttgacccca aaccagaacc 1621 agcagggaca gcaaacggat ccccttgtgg cagctgcagc agtgaattct gcccttgcat 1681 ttggacaagg tctggcagca ggcatgccag gttatccggt gttggctcct gctgcttact 1741 atgaccaaac tggtgccctt gtagtgaatg caggcgcgag aaatggtctt ggagctcctg 1801 ttcgacttgt agctcctgcc ccagtcatca ttagttcctc agctgcacaa gcagctgttg 1861 cagcagccgc agcttcagca aatggagcag ctggtggtct tgctggaaca acaaatggac 1921 catttcgccc tttaggaaca cagcagcctc agccccagcc ccagcagcag cccaataaca 1981 acctggcatc cagttctttc tacggcaaca actctctgaa cagcaattca cagagcagct 2041 ccctcttctc ccagggctct gcccagcctg ccaacacatc cttgggattc ggaagtagca 2101 gttctctcgg cgccaccctg ggatccgccc ttggagggtt tggaacagca gttgcaaact 2161 ccaacactgg cagtggctcc cgccgtgact ccctgactgg cagcagtgac ctttataaga 2221 ggacatcgag cagcttgacc cccattggac acagttttta taacggcctt agcttttcct 2281 cctctcctgg acccgtgggc atgcctctcc ctagtcaggg accaggacat tcacagacac 2341 cacctccttc cctctcttca catggatcct cttcaagctt aaacctggga ggactcacga 2401 acggcagtgg aagatacatc tctgctgctc caggcgctga agccaagtac cgcagtgcaa 2461 gcagcgcctc cagcctcttc agcccgagca gcactctttt ctcttcctct cgtttgcgat 2521 atggaatgtc tgatgtcatg ccttctggca ggagcaggct tttggaagat tttcgaaaca 2581 accggtaccc caatttacaa ctgcgggaga ttgctggaca tataatggaa ttttcccaag 2641 accagcatgg gtccagattc attcagctga aactggagcg tgccacacca gctgagcgcc 2701 agcttgtctt caatgaaatc ctccaggctg cctaccaact catggtggat gtgtttggta 2761 attacgtcat tcagaagttc tttgaatttg gcagtcttga acagaagctg gctttggcag 2821 aacggattcg aggccacgtc ctgtcattgg cactacagat gtatggctgc cgtgttatcc 2881 agaaagctct tgagtttatt ccttcagacc agcagaatga gatggttcgg gaactagatg 2941 gccatgtctt gaagtgtgtg aaagatcaga atggcaatca cgtggttcag aaatgcattg 3001 aatgtgtaca gccccagtct ttgcaattta tcatcgatgc gtttaaggga caggtatttg 3061 ccttatccac acatccttat ggctgccgag tgattcagag aatcctggag cactgtctcc 3121 ctgaccagac actccctatt ttagaggagc ttcaccagca cacagagcag cttgtacagg 3181 atcaatatgg aaattatgta atccaacatg tactggagca cggtcgtcct gaggataaaa 3241 gcaaaattgt agcagaaatc cgaggcaatg tacttgtatt gagtcagcac aaatttgcaa 3301 gcaatgttgt ggagaagtgt gttactcacg cctcacgtac ggagcgcgct gtgctcatcg 3361 atgaggtgtg caccatgaac gacggtcccc acagtgcctt atacaccatg atgaaggacc 3421 agtatgccaa ctacgtggtc cagaagatga ttgacgtggc ggagccaggc cagcggaaga 3481 tcgtcatgca taagatccgg ccccacatcg caactcttcg taagtacacc tatggcaagc 3541 acattctggc caagctggag aagtactaca tgaagaacgg tgttgactta gggcccatct 3601 gtggcccccc taatggtatc atctgcccaa ctttcttgta caaagtggtt ggtaagccta 3661 tccctaaccc tctcctcggt ctcgattcta cgtagtaatg agctagcgct aaccggtggc 3721 gcgttaagtc gacaatcaac ctctggatta