Construct: ORF TRCN0000488486

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF020372.1_s317c1
DNA Barcode:
GACCTCCTCAAGAGGCAACGCTGC
Epitope Tag:
V5 (not translated due to prior stop codon)
Notes:
Has stop codon in insert

Originally Annotated References:

Gene:
CAMK2B (816)

Vector Information:

Vector Backbone:
pLX_317
Pol II Cassette 1:
SV40-PuroR
Pol II Cassette 2:
EF1a-TRCN0000488486
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 816 CAMK2B calcium/calmodulin dependen... NM_001293170.2 100% 100%
2 human 816 CAMK2B calcium/calmodulin dependen... NM_172078.3 100% 100%
3 human 816 CAMK2B calcium/calmodulin dependen... XM_005249862.3 97.2% 97.2% 1130_1131ins45
4 human 816 CAMK2B calcium/calmodulin dependen... XM_006715784.2 95.5% 95.5% 1058_1059ins72
5 human 816 CAMK2B calcium/calmodulin dependen... NM_172079.2 95.5% 95.3% 947_947delCins73
6 human 816 CAMK2B calcium/calmodulin dependen... NM_172080.3 95.3% 95.3% 944_945ins75
7 human 816 CAMK2B calcium/calmodulin dependen... NM_172081.3 92.7% 92.6% 947_947delCins73;1058_1059ins45
8 human 816 CAMK2B calcium/calmodulin dependen... XM_017012664.1 92.6% 92.6% 944_945ins75;1055_1056ins45
9 human 816 CAMK2B calcium/calmodulin dependen... XM_005249864.3 91% 90.9% 947_947delCins73;986_987ins72
10 human 816 CAMK2B calcium/calmodulin dependen... XM_017012665.1 90.9% 90.9% 944_945ins75;983_984ins72
11 human 816 CAMK2B calcium/calmodulin dependen... NM_172082.3 90.7% 90.7% 1058_1059ins72;1229_1230ins78
12 human 816 CAMK2B calcium/calmodulin dependen... NM_172083.3 88.3% 88.1% 947_947delCins73;984_985ins117
13 human 816 CAMK2B calcium/calmodulin dependen... XM_024446945.1 86.3% 86.3% 1226_1483del
14 human 816 CAMK2B calcium/calmodulin dependen... NM_172084.3 82.8% 82.8% 944_945ins279
15 human 816 CAMK2B calcium/calmodulin dependen... XM_017012662.1 82.3% 82.3% 944_945ins75;1151_1408del
16 human 816 CAMK2B calcium/calmodulin dependen... XM_017012661.1 81.7% 78.5% (many diffs)
17 human 816 CAMK2B calcium/calmodulin dependen... NM_001220.5 81.3% 81.3% 1226_1597del
18 human 816 CAMK2B calcium/calmodulin dependen... XM_011515555.1 81.3% 81.3% 1224_1595del
19 human 816 CAMK2B calcium/calmodulin dependen... XM_011515553.1 80.7% 80.7% 1224_1610del
20 human 816 CAMK2B calcium/calmodulin dependen... XM_011515558.2 79.3% 67.5% (many diffs)
21 human 818 CAMK2G calcium/calmodulin dependen... NM_001204492.1 78.5% 85.4% (many diffs)
22 human 816 CAMK2B calcium/calmodulin dependen... XM_006715781.2 78.5% 75.3% (many diffs)
23 human 818 CAMK2G calcium/calmodulin dependen... NM_172169.3 77.4% 84.3% (many diffs)
24 human 816 CAMK2B calcium/calmodulin dependen... XM_011515549.1 77.2% 74.3% (many diffs)
25 human 818 CAMK2G calcium/calmodulin dependen... NM_001367543.1 77.2% 83.3% (many diffs)
26 human 818 CAMK2G calcium/calmodulin dependen... XM_006717998.1 77.2% 83.3% (many diffs)
27 human 816 CAMK2B calcium/calmodulin dependen... XM_011515547.1 76.4% 76.4% 1224_1724del
28 human 818 CAMK2G calcium/calmodulin dependen... NM_001367517.1 76.3% 83.7% (many diffs)
29 human 818 CAMK2G calcium/calmodulin dependen... NM_172170.5 76.3% 83.7% (many diffs)
30 human 818 CAMK2G calcium/calmodulin dependen... XM_024448218.1 76.2% 82.8% (many diffs)
31 human 816 CAMK2B calcium/calmodulin dependen... XM_017012666.2 76.1% 76.1% 0_1ins387
32 human 818 CAMK2G calcium/calmodulin dependen... XM_005270205.1 76% 84.1% (many diffs)
33 human 818 CAMK2G calcium/calmodulin dependen... NM_001367531.1 75.8% 81.7% (many diffs)
34 human 818 CAMK2G calcium/calmodulin dependen... XM_017016730.1 75.8% 81.7% (many diffs)
35 human 818 CAMK2G calcium/calmodulin dependen... NM_001367530.1 75.1% 82.6% (many diffs)
36 human 818 CAMK2G calcium/calmodulin dependen... NM_172173.3 75.1% 83.7% (many diffs)
37 human 818 CAMK2G calcium/calmodulin dependen... NM_001367532.1 75.1% 82.3% (many diffs)
38 human 818 CAMK2G calcium/calmodulin dependen... NM_001367516.1 74.6% 79% (many diffs)
39 human 818 CAMK2G calcium/calmodulin dependen... NM_001367527.1 74.1% 78.8% (many diffs)
40 human 818 CAMK2G calcium/calmodulin dependen... NM_001222.4 73.7% 82.5% (many diffs)
41 human 816 CAMK2B calcium/calmodulin dependen... XM_017012660.1 73.7% 70.8% (many diffs)
42 human 818 CAMK2G calcium/calmodulin dependen... NM_001367544.1 73.6% 80% (many diffs)
43 human 816 CAMK2B calcium/calmodulin dependen... XM_011515554.1 73.6% 70.8% (many diffs)
44 human 818 CAMK2G calcium/calmodulin dependen... NM_001367545.1 73.5% 77.2% (many diffs)
45 human 816 CAMK2B calcium/calmodulin dependen... XM_017012667.2 73.4% 73.4% 0_1ins387;743_744ins45
46 human 816 CAMK2B calcium/calmodulin dependen... XM_011515551.1 73% 73% 1058_1059ins72;1152_1652del
47 human 816 CAMK2B calcium/calmodulin dependen... XM_011515550.1 73% 72.9% 947_947delCins73;1152_1652del
48 human 816 CAMK2B calcium/calmodulin dependen... XM_011515552.1 72.9% 72.9% 944_945ins75;1149_1649del
49 human 818 CAMK2G calcium/calmodulin dependen... NM_001367546.1 72.6% 79% (many diffs)
50 human 818 CAMK2G calcium/calmodulin dependen... XM_005270198.1 72.5% 80% (many diffs)
51 human 818 CAMK2G calcium/calmodulin dependen... NM_001367534.1 72.5% 78.2% (many diffs)
52 human 818 CAMK2G calcium/calmodulin dependen... NM_001367548.1 72.4% 78.2% (many diffs)
53 human 816 CAMK2B calcium/calmodulin dependen... XM_017012669.2 71.7% 71.7% 0_1ins387;671_672ins72
54 human 816 CAMK2B calcium/calmodulin dependen... XM_017012668.2 71.7% 71.5% 0_1ins387;560_560delCins73
55 human 816 CAMK2B calcium/calmodulin dependen... XM_024446946.1 71.5% 71.5% 0_1ins387;557_558ins75
56 human 818 CAMK2G calcium/calmodulin dependen... NM_172171.2 71.5% 78.4% (many diffs)
57 human 818 CAMK2G calcium/calmodulin dependen... NM_001367547.1 71.4% 77% (many diffs)
58 human 815 CAMK2A calcium/calmodulin dependen... NM_001369025.2 71.2% 75.5% (many diffs)
59 human 815 CAMK2A calcium/calmodulin dependen... NM_001363990.1 71.1% 75.5% (many diffs)
60 human 815 CAMK2A calcium/calmodulin dependen... NM_171825.2 71.1% 75.5% (many diffs)
61 human 818 CAMK2G calcium/calmodulin dependen... XM_006717996.1 71.1% 76.6% (many diffs)
62 human 818 CAMK2G calcium/calmodulin dependen... NM_001367518.1 70.4% 77.4% (many diffs)
63 human 818 CAMK2G calcium/calmodulin dependen... NM_001367533.1 70.4% 78.4% (many diffs)
64 human 818 CAMK2G calcium/calmodulin dependen... NM_001367525.1 70.4% 77.1% (many diffs)
65 human 816 CAMK2B calcium/calmodulin dependen... XM_011515556.1 69.6% 69.5% 947_947delCins73;986_987ins72;1080_1580del
66 human 816 CAMK2B calcium/calmodulin dependen... XM_011515557.1 69.5% 69.5% 944_945ins75;983_984ins72;1077_1577del
67 human 816 CAMK2B calcium/calmodulin dependen... XM_017012670.2 68.9% 68.8% 0_1ins387;560_560delCins73;671_672ins45
68 human 818 CAMK2G calcium/calmodulin dependen... XM_024448219.1 64.4% 69.6% (many diffs)
69 human 818 CAMK2G calcium/calmodulin dependen... NM_001367539.1 60.4% 65.2% (many diffs)
70 human 816 CAMK2B calcium/calmodulin dependen... XM_011515559.2 58.2% 58.2% 0_1ins387;837_1337del
71 human 816 CAMK2B calcium/calmodulin dependen... XM_017012663.2 54.8% 54.8% 0_1ins387;671_672ins72;765_1265del
72 mouse 12323 Camk2b calcium/calmodulin-dependen... NM_007595.5 92.6% 99.2% (many diffs)
73 mouse 12323 Camk2b calcium/calmodulin-dependen... XM_017314239.1 92.4% 99% (many diffs)
74 mouse 12323 Camk2b calcium/calmodulin-dependen... XM_006514475.1 89.9% 96.4% (many diffs)
75 mouse 12323 Camk2b calcium/calmodulin-dependen... XM_017314240.1 89.7% 96.3% (many diffs)
76 mouse 12323 Camk2b calcium/calmodulin-dependen... XM_006514476.1 88.1% 94.6% (many diffs)
77 mouse 12323 Camk2b calcium/calmodulin-dependen... XM_006514477.1 88% 94.6% (many diffs)
78 mouse 12323 Camk2b calcium/calmodulin-dependen... NM_001174054.1 86.3% 92.7% (many diffs)
79 mouse 12323 Camk2b calcium/calmodulin-dependen... XM_006514479.2 85.4% 91.8% (many diffs)
80 mouse 12323 Camk2b calcium/calmodulin-dependen... NM_001174053.1 79% 85.1% (many diffs)
81 mouse 12323 Camk2b calcium/calmodulin-dependen... XM_017314237.1 76.1% 78.4% (many diffs)
82 mouse 12323 Camk2b calcium/calmodulin-dependen... XM_006514465.2 75.3% 80.7% (many diffs)
83 mouse 12323 Camk2b calcium/calmodulin-dependen... XM_006514464.2 75.2% 80.6% (many diffs)
84 mouse 12325 Camk2g calcium/calmodulin-dependen... NM_001039138.2 74.9% 83.7% (many diffs)
85 mouse 12325 Camk2g calcium/calmodulin-dependen... XM_017315826.1 74.7% 84.1% (many diffs)
86 mouse 12323 Camk2b calcium/calmodulin-dependen... XM_006514469.2 72.5% 74.7% (many diffs)
87 mouse 12325 Camk2g calcium/calmodulin-dependen... NM_001039139.2 72.4% 82.5% (many diffs)
88 mouse 12323 Camk2b calcium/calmodulin-dependen... XM_006514470.3 72.4% 74.7% (many diffs)
89 mouse 12323 Camk2b calcium/calmodulin-dependen... XM_006514467.2 71.7% 77% (many diffs)
90 mouse 12323 Camk2b calcium/calmodulin-dependen... XM_017314238.1 71.6% 77% (many diffs)
91 mouse 12322 Camk2a calcium/calmodulin-dependen... XM_017317805.2 70.8% 75.5% (many diffs)
92 mouse 12322 Camk2a calcium/calmodulin-dependen... NM_177407.4 70.6% 75.5% (many diffs)
93 mouse 12325 Camk2g calcium/calmodulin-dependen... XM_006518492.2 70.4% 78.4% (many diffs)
94 mouse 12325 Camk2g calcium/calmodulin-dependen... XM_006518490.3 70% 76.6% (many diffs)
95 mouse 12325 Camk2g calcium/calmodulin-dependen... XM_006518495.2 69.3% 77.4% (many diffs)
96 mouse 12323 Camk2b calcium/calmodulin-dependen... XM_017314241.1 51.8% 51.1% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
72
ORF end:
1698
ORF length:
1626
Sequence:
1tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag
61caggcttcac catggccacc acggtgacct gcacccgctt caccgacgag taccagctct
121acgaggatat tggcaagggg gctttctctg tggtccgacg ctgtgtcaag ctctgcaccg
181gccatgagta tgcagccaag atcatcaaca ccaagaagct gtcagccaga gatcaccaga
241agctggagag agaggctcgg atctgccgcc ttctgaagca ttccaacatc gtgcgtctcc
301acgacagcat ctccgaggag ggcttccact acctggtctt cgatctggtc actggtgggg
361agctctttga agacattgtg gcgagagagt actacagcga ggctgatgcc agtcactgta
421tccagcagat cctggaggcc gttctccatt gtcaccaaat gggggtcgtc cacagagacc
481tcaagccgga gaacctgctt ctggccagca agtgcaaagg ggctgcagtg aagctggcag
541acttcggcct agctatcgag gtgcaggggg accagcaggc atggtttggt ttcgctggca
601caccaggcta cctgtcccct gaggtccttc gcaaagaggc gtatggcaag cctgtggaca
661tctgggcatg tggggtgatc ctgtacatcc tgctcgtggg ctacccaccc ttctgggacg
721aggaccagca caagctgtac cagcagatca aggctggtgc ctatgacttc ccgtcccctg
781agtgggacac cgtcactcct gaagccaaaa acctcatcaa ccagatgctg accatcaacc
841ctgccaagcg catcacagcc catgaggccc tgaagcaccc gtgggtctgc caacgctcca
901cggtagcatc catgatgcac agacaggaga ctgtggagtg tctgaaaaag ttcaatgcca
961ggagaaagct caagggagcc atcctcacca ccatgctggc cacacggaat ttctcagtgg
1021gcagacagac caccgctccg gccacaatgt ccaccgcggc ctccggcacc accatggggc
1081tggtggaaca agccaagagt ttactcaaca agaaagcaga tggagtcaag ccccagacga
1141atagcaccaa aaacagtgca gccgccacca gccccaaagg gacgcttcct cctgccgccc
1201tggagcctca aaccaccgtc atccataacc cagtggacgg gattaaggag tcttctgaca
1261gtgccaatac caccatagag gatgaagacg ctaaagcccg gaagcaggag atcattaaga
1321ccacggagca gctcatcgag gccgtcaaca acggtgactt tgaggcctac gcgaaaatct
1381gtgacccagg gctgacctcg tttgagcctg aagcactggg caacctggtt gaagggatgg
1441acttccacag attctacttc gagaacctgc tggccaagaa cagcaagccg atccacacga
1501ccatcctgaa cccacacgtg cacgtcattG GAGAGGATGC CGCCTGCATC GCTTACATCC
1561GGCTCACGCA GTACATTGAC GGGCAGGGCC GGCCCCGCAC CAGCCAGTCT GAGGAGACCC
1621GCGTGTGGCA CCGCCGCGAC GGCAAGTGGC AGAACGTGCA CTTCCACTGC TCGGGCGCGC
1681CTGTGGCCCC GCTGCAGTAG GACCCAGCTT TCTTGTACAA AGTGGTTGAT ATCGGTAAGC
1741CTATCCCTAA CCCTCTCCTC GGTCTCGATT CTACGTAGTA ATGAACTAGT CCGTAACTTG
1801AAAGTATTTC GATTTCTTGG CTTTATATAT CTTGTGGAAA GGACGAGACC TCCTCAAGAG
1861GCAACGCTGC ACGCGTTAAG TCgacaatca acctctggat tacaaaattt gtgaaagatt

Download FASTA (ORF) (Full)