Construct: ORF TRCN0000489543

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF021904.1_s317c1
DNA Barcode:
ACACCATGAACCACCTGATCTCCA
Epitope Tag:
V5 (not translated due to prior stop codon)
Notes:
Early stop codon detected

Originally Annotated References:

Gene:
RSRC2 (65117)

Vector Information:

Vector Backbone:
pLX_317
Pol II Cassette 1:
SV40-PuroR
Pol II Cassette 2:
EF1a-TRCN0000489543
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 65117 RSRC2 arginine and serine rich co... NM_023012.6 93% 0_1ins96;671C>G
2 human 65117 RSRC2 arginine and serine rich co... XM_005253601.2 92.8% 0_1ins96;671C>G;802_804delGCA
3 human 65117 RSRC2 arginine and serine rich co... XM_017019830.2 86.6% 0_1ins96;671C>G;1034_1035ins90
4 human 65117 RSRC2 arginine and serine rich co... XM_011538688.2 86.4% (many diffs)
5 human 65117 RSRC2 arginine and serine rich co... XM_005253602.2 79.7% (many diffs)
6 human 65117 RSRC2 arginine and serine rich co... XM_017019831.1 79.7% (many diffs)
7 human 65117 RSRC2 arginine and serine rich co... XM_005253604.2 78.8% (many diffs)
8 human 65117 RSRC2 arginine and serine rich co... XM_011538689.1 78.8% (many diffs)
9 human 65117 RSRC2 arginine and serine rich co... XR_001748848.2 58.4% (many diffs)
10 human 65117 RSRC2 arginine and serine rich co... XR_002957370.1 58.3% (many diffs)
11 human 65117 RSRC2 arginine and serine rich co... XR_001748845.2 56.2% (many diffs)
12 human 65117 RSRC2 arginine and serine rich co... XR_001748844.2 56.1% (many diffs)
13 human 65117 RSRC2 arginine and serine rich co... XR_001748847.2 55.8% (many diffs)
14 human 65117 RSRC2 arginine and serine rich co... XR_001748846.2 55.7% (many diffs)
15 human 65117 RSRC2 arginine and serine rich co... XM_017019833.2 43.3% 0_1ins792
16 human 65117 RSRC2 arginine and serine rich co... XM_017019832.1 43.2% 0_1ins792;106_108delGCA
17 human 65117 RSRC2 arginine and serine rich co... NR_036436.2 38.1% (many diffs)
18 human 65117 RSRC2 arginine and serine rich co... NR_036434.2 37.5% (many diffs)
19 human 65117 RSRC2 arginine and serine rich co... NR_036435.2 37.3% (many diffs)
20 mouse 208606 Rsrc2 arginine/serine-rich coiled... XM_006530240.3 83.7% (many diffs)
21 mouse 208606 Rsrc2 arginine/serine-rich coiled... NM_001005525.2 83.5% (many diffs)
22 mouse 208606 Rsrc2 arginine/serine-rich coiled... XM_006530244.3 72% (many diffs)
23 mouse 208606 Rsrc2 arginine/serine-rich coiled... XM_006530241.3 71.8% (many diffs)
24 mouse 208606 Rsrc2 arginine/serine-rich coiled... XM_006530242.3 71.8% (many diffs)
25 mouse 208606 Rsrc2 arginine/serine-rich coiled... XM_006530243.3 71.8% (many diffs)
26 mouse 208606 Rsrc2 arginine/serine-rich coiled... XM_011248190.2 71.2% (many diffs)
27 mouse 208606 Rsrc2 arginine/serine-rich coiled... XM_017320778.1 71.2% (many diffs)
28 mouse 208606 Rsrc2 arginine/serine-rich coiled... XM_017320779.1 71.2% (many diffs)
29 mouse 208606 Rsrc2 arginine/serine-rich coiled... NM_025438.2 71% (many diffs)
30 mouse 208606 Rsrc2 arginine/serine-rich coiled... XM_006530246.3 71% (many diffs)
31 mouse 208606 Rsrc2 arginine/serine-rich coiled... XM_006530247.3 71% (many diffs)
32 mouse 208606 Rsrc2 arginine/serine-rich coiled... XR_001784647.1 53.9% (many diffs)
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Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
65
ORF end:
77
ORF length:
12
Sequence:
1tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaccca
61aataatgatt ttattttgac tgatagtgac ctgttcgttg caacaaattg atgagcaatg
121cttttttata atgccaactt tgtacaaaaa agcaggcacc atggcggcta gtgatacaga
181gcgagatgga ctagccccag aaaagacatc accagataga gataagaaaa aagagcagtc
241agaagtatct gtttctccta gagcttcaaa acatcattat tcaagatcac gatcaaggtc
301aagagaaaga aaacgaaagt cagataatga aggaagaaaa cacaggagcc ggagcagaag
361caaagaggga agaagacatg aatccaaaga taaatcctct aagaaacata agtctgagga
421acataatgac aaagaacatt cttctgataa aggaagagag cgactaaatt catctgaaaa
481tggtgaggac aggcacaaac gcaaagaaag aaagtcatca agaggcagaa gtcactcaag
541atctaggtct cgtgaaagac gccatcgtag tagaagcagg gagcggaaga agtctcgatc
601caggagtagg gagcggaaga aatcgagatc cagaagcaga gagaggaaga aatcgagatc
661cagaagcagg gaaagaaaac ggcggatcag gtctcgttcc cgctcaagat caagacacag
721gcataggact agaagcagga gtaggacaag gagtaggagt cgagatagaa agaagagaat
781tgaaaagccg agaagattta gcagaagttt aagccggact ccaagtccac gtcccttcag
841aggcagaaac acagcaatgg atgcacagga agctttagct agaaggttgg aaagggcaaa
901gaaattacaa gaacagcgag aaaaggaaat ggttgaaaaa caaaaacaac aagaaatagc
961tgcagctgca gctactggag gttctgttct caatgttgct gccctgttgg catcaggaac
1021acaagtaaca cctcagatag ccatggcagc tcagatggca gccctGCAAG CTAAAGCTTT
1081GGCAGAGACA GGAATAGCTG TTCCTAGCTA CTATAACCCA GCCGCTGTTA ATCCAATGAA
1141ATTTGCTGAA CAAGAGAAAA AAAGGAAAAT GCTTTGGCAG GGCAAGAAAG AAGGGGACAA
1201ATCCCAATCT GCTGAAATAT GGGAAAAATT GAATTTTGGA AACAAGGACC AAAATGTCAA
1261ATTTAGGAAA TTGATGGGTA TTAAGAGTGA AGATGAAGCT GGATGTAGCT CAGTTGATGA
1321AGAAAGTTAC AAGACTCTGA AGCAGCAGGA AGAAGTATTT CGAAATTTAG ATGCTCAGTA
1381TGAAATGGCA AGATCACAAA CCCACACACA AAGAGGAATG GGTTTGGGTT TCACATCTTC
1441AATGCGAGGA ATGGATGCAG TTTAGAACCC AGCTTTCTTG TACAAAGTGG TTGATATCGG
1501TAAGCCTATC CCTAACCCTC TCCTCGGTCT CGATTCTACG TAGTAATGAA CTAGTCCGTA
1561ACTTGAAAGT ATTTCGATTT CTTGGCTTTA TATATCTTGT GGAAAGGACG AACACCATGA
1621ACCACCTGAT CTCCAACGCG TTAAGTCgac aatcaacctc tggattacaa aatttgtgaa
1681agatt

Download FASTA (ORF) (Full)