Construct: ORF TRCN0000471554
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF014009.1_s317c1
- Derived from:
- ccsbBroadEn_11149
- DNA Barcode:
- TAGAGCTACCAGGTATCCCGCGCG
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- SOX5 (6660)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000471554
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
---|---|---|---|---|---|---|---|---|
1 | human | 6660 | SOX5 | SRY-box transcription factor 5 | NM_001261414.3 | 100% | 100% | |
2 | human | 6660 | SOX5 | SRY-box transcription factor 5 | NM_152989.5 | 85.6% | 85.6% | 1123_1446del |
3 | human | 6660 | SOX5 | SRY-box transcription factor 5 | XM_017019896.1 | 85.6% | 85.6% | 1123_1446del |
4 | human | 6660 | SOX5 | SRY-box transcription factor 5 | XM_024449160.1 | 85.6% | 85.6% | 1123_1446del |
5 | human | 6660 | SOX5 | SRY-box transcription factor 5 | XM_024449161.1 | 85.6% | 85.6% | 1123_1446del |
6 | human | 6660 | SOX5 | SRY-box transcription factor 5 | XM_011520834.2 | 85.4% | 85.4% | 769_771delCAG;1126_1449del |
7 | human | 6660 | SOX5 | SRY-box transcription factor 5 | XM_011520835.2 | 85.4% | 85.4% | 769_771delCAG;1126_1449del |
8 | human | 6660 | SOX5 | SRY-box transcription factor 5 | XM_011520837.2 | 85.4% | 85.4% | 769_771delCAG;1126_1449del |
9 | human | 6660 | SOX5 | SRY-box transcription factor 5 | XM_017019890.1 | 85.4% | 85.4% | 769_771delCAG;1126_1449del |
10 | human | 6660 | SOX5 | SRY-box transcription factor 5 | XM_017019891.1 | 85.4% | 85.4% | 769_771delCAG;1126_1449del |
11 | human | 6660 | SOX5 | SRY-box transcription factor 5 | XM_017019892.1 | 85.4% | 85.4% | 769_771delCAG;1126_1449del |
12 | human | 6660 | SOX5 | SRY-box transcription factor 5 | XM_017019893.1 | 85.4% | 85.4% | 769_771delCAG;1126_1449del |
13 | human | 6660 | SOX5 | SRY-box transcription factor 5 | XM_017019894.1 | 85.4% | 85.4% | 769_771delCAG;1126_1449del |
14 | human | 6660 | SOX5 | SRY-box transcription factor 5 | XM_017019895.1 | 85.4% | 85.4% | 769_771delCAG;1126_1449del |
15 | human | 6660 | SOX5 | SRY-box transcription factor 5 | XM_024449150.1 | 85.4% | 85.4% | 769_771delCAG;1126_1449del |
16 | human | 6660 | SOX5 | SRY-box transcription factor 5 | XM_024449151.1 | 85.4% | 85.4% | 769_771delCAG;1126_1449del |
17 | human | 6660 | SOX5 | SRY-box transcription factor 5 | XM_024449152.1 | 85.4% | 85.4% | 769_771delCAG;1126_1449del |
18 | human | 6660 | SOX5 | SRY-box transcription factor 5 | XM_024449153.1 | 85.4% | 85.4% | 769_771delCAG;1126_1449del |
19 | human | 6660 | SOX5 | SRY-box transcription factor 5 | XM_024449154.1 | 85.4% | 85.4% | 769_771delCAG;1126_1449del |
20 | human | 6660 | SOX5 | SRY-box transcription factor 5 | XM_024449155.1 | 85.4% | 85.4% | 769_771delCAG;1126_1449del |
21 | human | 6660 | SOX5 | SRY-box transcription factor 5 | XM_024449157.1 | 85.4% | 85.4% | 769_771delCAG;1126_1449del |
22 | human | 6660 | SOX5 | SRY-box transcription factor 5 | XM_024449158.1 | 85.4% | 85.4% | 769_771delCAG;1126_1449del |
23 | human | 6660 | SOX5 | SRY-box transcription factor 5 | XM_024449159.1 | 85.4% | 85.4% | 769_771delCAG;1126_1449del |
24 | human | 6660 | SOX5 | SRY-box transcription factor 5 | NM_001261415.2 | 85.2% | 85.2% | 1_9delATGTCTGTG;1132_1455del |
25 | human | 6660 | SOX5 | SRY-box transcription factor 5 | XM_011520833.2 | 85.1% | 85.1% | 1_9delATGTCTGTG;778_780delCAG;1135_1458del |
26 | human | 6660 | SOX5 | SRY-box transcription factor 5 | NM_006940.6 | 84.1% | 84.1% | 1_39del;1162_1485del |
27 | human | 6660 | SOX5 | SRY-box transcription factor 5 | XM_011520832.2 | 84% | 84% | 1_39del;808_810delCAG;1165_1488del |
28 | human | 6660 | SOX5 | SRY-box transcription factor 5 | XM_017019898.1 | 80.9% | 80.9% | 126_127ins105;1018_1341del |
29 | human | 6660 | SOX5 | SRY-box transcription factor 5 | XM_017019899.1 | 80.9% | 80.9% | 126_127ins105;1018_1341del |
30 | human | 6660 | SOX5 | SRY-box transcription factor 5 | XM_017019900.1 | 80.9% | 80.9% | 126_127ins105;1018_1341del |
31 | human | 6660 | SOX5 | SRY-box transcription factor 5 | XM_017019901.1 | 80.9% | 80.9% | 126_127ins105;1018_1341del |
32 | human | 6660 | SOX5 | SRY-box transcription factor 5 | XM_017019897.1 | 80.8% | 80.8% | 126_127ins105;664_666delCAG;1021_1344del |
33 | human | 6660 | SOX5 | SRY-box transcription factor 5 | XM_024449163.1 | 80.8% | 80.8% | 126_127ins105;664_666delCAG;1021_1344del |
34 | human | 6660 | SOX5 | SRY-box transcription factor 5 | XM_024449164.1 | 80.8% | 80.8% | 126_127ins105;664_666delCAG;1021_1344del |
35 | human | 6660 | SOX5 | SRY-box transcription factor 5 | XM_024449165.1 | 80.8% | 80.8% | 126_127ins105;664_666delCAG;1021_1344del |
36 | human | 6660 | SOX5 | SRY-box transcription factor 5 | NM_001330785.2 | 79.5% | 79.5% | 1_39del;165_166ins105;1057_1380del |
37 | human | 6660 | SOX5 | SRY-box transcription factor 5 | XM_011520838.2 | 79.4% | 79.4% | (many diffs) |
38 | human | 6660 | SOX5 | SRY-box transcription factor 5 | XM_017019889.1 | 73.3% | 73.3% | 1_231del;357_358ins105;1249_1572del |
39 | human | 6660 | SOX5 | SRY-box transcription factor 5 | XM_017019888.1 | 73.3% | 73.3% | (many diffs) |
40 | human | 6660 | SOX5 | SRY-box transcription factor 5 | XM_011520842.2 | 57.5% | 40.9% | (many diffs) |
41 | human | 6660 | SOX5 | SRY-box transcription factor 5 | XM_017019903.1 | 57.5% | 57.2% | (many diffs) |
42 | human | 6660 | SOX5 | SRY-box transcription factor 5 | XM_017019902.1 | 57.4% | 57.1% | (many diffs) |
43 | human | 6660 | SOX5 | SRY-box transcription factor 5 | NM_178010.4 | 52.1% | 41.6% | (many diffs) |
44 | mouse | 20678 | Sox5 | SRY (sex determining region... | XM_011241582.1 | 81.2% | 83.8% | (many diffs) |
45 | mouse | 20678 | Sox5 | SRY (sex determining region... | NM_001113559.2 | 79.6% | 82.2% | (many diffs) |
46 | mouse | 20678 | Sox5 | SRY (sex determining region... | XM_006506936.3 | 77.3% | 82.9% | (many diffs) |
47 | mouse | 20678 | Sox5 | SRY (sex determining region... | XM_006506937.3 | 77.3% | 82.9% | (many diffs) |
48 | mouse | 20678 | Sox5 | SRY (sex determining region... | XM_011241577.1 | 77.3% | 82.9% | (many diffs) |
49 | mouse | 20678 | Sox5 | SRY (sex determining region... | XM_011241578.1 | 77.3% | 82.9% | (many diffs) |
50 | mouse | 20678 | Sox5 | SRY (sex determining region... | XM_011241579.1 | 77.3% | 82.9% | (many diffs) |
51 | mouse | 20678 | Sox5 | SRY (sex determining region... | XM_006506938.3 | 76.6% | 79.1% | (many diffs) |
52 | mouse | 20678 | Sox5 | SRY (sex determining region... | XM_017321476.1 | 76.6% | 79.1% | (many diffs) |
53 | mouse | 20678 | Sox5 | SRY (sex determining region... | NM_011444.3 | 76.1% | 81.6% | (many diffs) |
54 | mouse | 20678 | Sox5 | SRY (sex determining region... | XM_006506933.3 | 76% | 81.5% | (many diffs) |
55 | mouse | 20678 | Sox5 | SRY (sex determining region... | NM_001243163.1 | 75.2% | 77.7% | (many diffs) |
56 | mouse | 20678 | Sox5 | SRY (sex determining region... | XM_017321475.1 | 75.1% | 77.6% | (many diffs) |
57 | mouse | 20678 | Sox5 | SRY (sex determining region... | XM_006506932.3 | 73.4% | 78.7% | (many diffs) |
58 | mouse | 20678 | Sox5 | SRY (sex determining region... | XM_006506931.3 | 73.3% | 78.6% | (many diffs) |
59 | mouse | 20678 | Sox5 | SRY (sex determining region... | XM_006506941.3 | 73.1% | 78.6% | (many diffs) |
60 | mouse | 20678 | Sox5 | SRY (sex determining region... | XM_017321474.1 | 73.1% | 78.6% | (many diffs) |
61 | mouse | 20678 | Sox5 | SRY (sex determining region... | XM_006506940.3 | 73% | 78.5% | (many diffs) |
62 | mouse | 20678 | Sox5 | SRY (sex determining region... | XM_011241581.1 | 73% | 78.5% | (many diffs) |
63 | mouse | 20678 | Sox5 | SRY (sex determining region... | XM_006506942.3 | 72.4% | 74.7% | (many diffs) |
64 | mouse | 20678 | Sox5 | SRY (sex determining region... | XM_011241580.2 | 71.8% | 77.3% | (many diffs) |
65 | mouse | 20678 | Sox5 | SRY (sex determining region... | XM_006506939.3 | 71.7% | 77.2% | (many diffs) |
66 | mouse | 20678 | Sox5 | SRY (sex determining region... | XM_006506935.3 | 69.3% | 74.5% | (many diffs) |
67 | mouse | 20678 | Sox5 | SRY (sex determining region... | XM_006506934.3 | 69.2% | 74.4% | (many diffs) |
68 | mouse | 20678 | Sox5 | SRY (sex determining region... | NM_001347506.1 | 47.2% | 41.2% | (many diffs) |
69 | mouse | 20678 | Sox5 | SRY (sex determining region... | XM_006506944.3 | 43.3% | 45.2% | (many diffs) |
70 | mouse | 20678 | Sox5 | SRY (sex determining region... | XM_017321477.1 | 43.3% | 45.2% | (many diffs) |
71 | mouse | 20678 | Sox5 | SRY (sex determining region... | XM_006506945.3 | 40.1% | 43.2% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1992
- ORF length:
- 1926
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgtc ttccaagcga ccagcctctc cgtatgggga agcagatgga gaggtagcca 121 tggtgacaag cagacagaaa gtggaagaag aggagagtga cgggctccca gcctttcacc 181 ttcccttgca tgtgagtttt cccaacaagc ctcactctga ggaatttcag ccagtttctc 241 tgctgacgca agagacttgt ggccatagga ctcccacttc tcagcacaat acaatggaag 301 ttgatggcaa taaagttatg tcttcatttg ccccacacaa ctcatctacc tcacctcaga 361 aggcagaaga aggtgggcga cagagtggcg agtccttgtc tagtacagcc ctgggaactc 421 ctgaacggcg caagggcagt ttagctgatg ttgttgacac cttgaagcag aggaaaatgg 481 aagagctcat caaaaacgag ccggaagaaa cccccagtat tgaaaaacta ctctcaaagg 541 actggaaaga caagcttctt gcaatgggat cggggaactt tggcgaaata aaagggactc 601 ccgagagctt agctgagaaa gaaaggcaac tcatgggtat gatcaaccag ctgaccagcc 661 tccgagagca gctgttggct gcccacgatg agcagaagaa actagctgcc tctcagattg 721 agaaacagcg tcagcaaatg gagctggcca agcagcaaca agaacaaatt gcaagacagc 781 agcagcagct tctacagcaa caacacaaaa tcaatttgct ccagcaacag atccaggttc 841 aaggtcagct gccgccatta atgattcccg tattccctcc tgatcaacgg acactggctg 901 cagctgccca gcaaggattc ctcctccctc caggcttcag ctataaggct ggatgtagtg 961 acccttaccc tgttcagctg atcccaacta ccatggcagc tgctgccgca gcaacaccag 1021 gcttaggccc actccaactg cagcagttat atgctgccca gctagctgca atgcaggtat 1081 ctccaggagg gaagctgcca ggcatacccc aaggcaacct tggtgctgct gtatctccta 1141 ccagcattca cacagacaag agcacaaaca gcccaccacc caaaagcaag gaaaaaacaa 1201 cactggagag tctgactcag caactggcag ttaaacagaa tgaagaagga aaatttagcc 1261 atgcaatgat ggatttcaat ctgagtggag attctgatgg aagtgctgga gtctcagagt 1321 caagaattta tagggaatcc cgagggcgtg gtagcaatga accccacata aagcgtccaa 1381 tgaatgcctt catggtgtgg gctaaagatg aacggagaaa gatccttcaa gcctttcctg 1441 acatgcacaa ctccaacatc agcaagatat tgggatctcg ctggaaagct atgacaaacc 1501 tagagaaaca gccatattat gaggagcaag cccgtctcag caagcagcac ctggagaagt 1561 accctgacta taagtacaag ccCAGGCCAA AGCGCACCTG CCTGGTGGAT GGCAAAAAGC 1621 TGCGCATTGG TGAATACAAG GCAATCATGC GCAACAGGCG GCAGGAAATG CGGCAGTACT 1681 TCAATGTTGG GCAACAAGCA CAGATCCCCA TTGCCACTGC TGGTGTTGTG TACCCTGGAG 1741 CCATCGCCAT GGCTGGGATG CCCTCCCCTC ACCTGCCCTC GGAGCACTCA AGCGTGTCTA 1801 GCAGCCCAGA GCCTGGGATG CCTGTTATCC AGAGCACTTA CGGTGTGAAA GGAGAGGAGC 1861 CACATATCAA AGAAGAGATA CAGGCCGAGG ACATCAATGG AGAAATTTAT GATGAGTACG 1921 ACGAGGAAGA GGATGATCCA GATGTAGATT ATGGGAGTGA CAGTGAAAAC CATATTGCAG 1981 GACAAGCCAA CTGCCCAACT TTCTTGTACA AAGTGGTTGA TATCGGTAAG CCTATCCCTA 2041 ACCCTCTCCT CGGTCTCGAT TCTACGTAGT AATGAACTAG TCCGTAACTT GAAAGTATTT 2101 CGATTTCTTG GCTTTATATA TCTTGTGGAA AGGACGATAG AGCTACCAGG TATCCCGCGC 2161 GACGCGTTAA GTCgacaatc aacctctgga ttacaaaatt tgtgaaagat t