Construct: ORF ccsbBroadEn_15487

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF013755.1_s300c1, BRDN0000393422
DNA Barcode:
None
Epitope Tag:
None
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
KRAS (3845)

Vector Information:

Vector Backbone:
pDONR223
Pol II Cassette 1:
n/a
Pol II Cassette 2:
n/a
Selection Marker:
n/a
Visible Reporter:
n/a
Epitope Tag:
n/a

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 8091 HMGA2 high mobility group AT-hook 2 NM_001300919.1 36.7% 31.9% (many diffs)
2 human 124801 LSM12 LSM12 homolog NM_001369484.1 20.4% 5.2% (many diffs)
3 human 100129940 HMGA2-AS1 HMGA2 antisense RNA 1 NR_158985.1 13.5% (many diffs)
4 human 100652758 LOC100652758 uncharacterized LOC100652758 NR_132346.1 13.5% (many diffs)
5 human 104472718 LINC00508 long intergenic non-protein... NR_126452.2 11.1% (many diffs)
6 human 100652758 LOC100652758 uncharacterized LOC100652758 NR_132345.1 10.9% (many diffs)
7 human 107984334 LOC107984334 uncharacterized LOC107984334 XR_001748245.1 10.8% (many diffs)
8 human 135398 C6orf141 chromosome 6 open reading f... NR_146855.2 9.5% (many diffs)
9 human 101928809 LOC101928809 uncharacterized LOC101928809 NR_125906.1 9.4% (many diffs)
10 human 100885795 LARS2-AS1 LARS2 antisense RNA 1 NR_048543.1 8.7% (many diffs)
11 human 643770 LINC02453 long intergenic non-protein... NR_038383.1 8.6% (many diffs)
12 human 105376412 LOC105376412 uncharacterized LOC105376412 XR_930661.2 8.4% (many diffs)
13 human 101928847 LOC101928847 uncharacterized LOC101928847 NR_120563.1 8.4% (many diffs)
14 human 101927257 MRTFA-AS1 MRTFA antisense RNA 1 NR_109965.1 8.3% (many diffs)
15 human 101927245 LOC101927245 uncharacterized LOC101927245 NR_160672.1 8.2% (many diffs)
16 human 102659353 THRIL TNF and HNRNPL related immu... NR_110375.1 8.1% (many diffs)
17 human 9326 ZNHIT3 zinc finger HIT-type contai... NR_104011.2 8.1% (many diffs)
18 human 5820 PVT1 Pvt1 oncogene NR_003367.3 8% (many diffs)
19 human 285972 LINC00996 long intergenic non-protein... NR_034033.1 7.9% (many diffs)
20 human 11092 SPACA9 sperm acrosome associated 9 NR_133631.2 7.9% (many diffs)
21 human 105376412 LOC105376412 uncharacterized LOC105376412 XR_002957063.1 7.8% (many diffs)
22 human 126661 CCDC163 coiled-coil domain containi... NR_152413.2 7.7% (many diffs)
23 human 128989 TANGO2 transport and golgi organiz... XR_002958662.1 7.5% (many diffs)
24 human 54108 CHRAC1 chromatin accessibility com... NR_040712.2 7.4% (many diffs)
25 human 100506334 LINC00649 long intergenic non-protein... NR_134558.1 7.4% (many diffs)
26 human 128989 TANGO2 transport and golgi organiz... XR_001755162.1 7.3% (many diffs)
27 human 126661 CCDC163 coiled-coil domain containi... NR_152412.2 7.2% (many diffs)
28 human 128989 TANGO2 transport and golgi organiz... XR_001755161.1 7.2% (many diffs)
29 human 102724015 LOC102724015 uncharacterized LOC102724015 XR_001750808.2 7.2% (many diffs)
30 human 128989 TANGO2 transport and golgi organiz... XR_001755164.1 7.2% (many diffs)
31 human 112268295 LOC112268295 uncharacterized LOC112268295 XR_002958751.1 7.1% (many diffs)
32 human 9616 RNF7 ring finger protein 7 NR_037703.1 7.1% (many diffs)
33 human 9616 RNF7 ring finger protein 7 NR_037702.1 7.1% (many diffs)
34 human 126661 CCDC163 coiled-coil domain containi... NR_152411.2 7% (many diffs)
35 human 126661 CCDC163 coiled-coil domain containi... NR_033297.3 7% (many diffs)
36 human 135398 C6orf141 chromosome 6 open reading f... NR_146858.2 7% (many diffs)
37 human 284865 LOC284865 uncharacterized LOC284865 NR_038460.2 6.9% (many diffs)
38 human 54108 CHRAC1 chromatin accessibility com... NR_023360.3 6.9% (many diffs)
39 human 100996251 LRIG2-DT LRIG2 divergent transcript NR_103777.1 6.8% (many diffs)
40 human 126661 CCDC163 coiled-coil domain containi... XR_002959324.1 6.8% (many diffs)
41 human 128989 TANGO2 transport and golgi organiz... XR_001755163.1 6.8% (many diffs)
42 human 101929823 LOC101929823 uncharacterized LOC101929823 XR_942199.3 6.8% (many diffs)
43 human 101929823 LOC101929823 uncharacterized LOC101929823 XR_952122.3 6.8% (many diffs)
44 human 101929823 LOC101929823 uncharacterized LOC101929823 XR_942198.3 6.8% (many diffs)
45 human 101929823 LOC101929823 uncharacterized LOC101929823 XR_952121.3 6.8% (many diffs)
46 human 126661 CCDC163 coiled-coil domain containi... NR_152414.2 6.8% (many diffs)
47 human 126661 CCDC163 coiled-coil domain containi... NR_033298.3 6.7% (many diffs)
48 human 135398 C6orf141 chromosome 6 open reading f... NR_146853.2 6.7% (many diffs)
49 human 9753 ZSCAN12 zinc finger and SCAN domain... NR_160527.1 6.4% (many diffs)
50 human 283089 WDR11-AS1 WDR11 antisense RNA 1 NR_033850.1 6.4% (many diffs)
51 human 147276 C18orf15 chromosome 18 open reading ... NR_146617.1 6.3% (many diffs)
52 human 93349 SP140L SP140 nuclear body protein ... NR_148198.1 6.2% (many diffs)
53 human 9753 ZSCAN12 zinc finger and SCAN domain... NR_136513.2 6.2% (many diffs)
54 human 135398 C6orf141 chromosome 6 open reading f... NR_146863.2 6.2% (many diffs)
55 human 399886 LINC02687 long intergenic non-protein... NR_122071.1 6.1% (many diffs)
56 human 9753 ZSCAN12 zinc finger and SCAN domain... NR_133668.1 6.1% (many diffs)
57 human 101928104 LINC01482 long intergenic non-protein... NR_110825.1 6.1% (many diffs)
58 human 105369347 RELA-DT RELA divergent transcript XR_002957252.1 6% (many diffs)
59 human 9753 ZSCAN12 zinc finger and SCAN domain... NR_133669.1 5.9% (many diffs)
60 human 440600 RBM15-AS1 RBM15 antisense RNA 1 NR_036595.1 5.9% (many diffs)
61 human 112268202 LOC112268202 uncharacterized LOC112268202 XR_002958144.1 5.9% (many diffs)
62 human 51379 CRLF3 cytokine receptor like fact... XR_001752525.2 5.9% (many diffs)
63 human 51379 CRLF3 cytokine receptor like fact... NR_073118.1 5.8% (many diffs)
64 human 100506334 LINC00649 long intergenic non-protein... NR_038883.2 5.8% (many diffs)
65 human 105373328 LOC105373328 uncharacterized LOC105373328 XR_938573.3 5.8% (many diffs)
66 human 107986847 LOC107986847 uncharacterized LOC107986847 XR_001745359.2 5.7% (many diffs)
67 human 93349 SP140L SP140 nuclear body protein ... XR_001739075.1 5.7% (many diffs)
68 human 105371800 LOC105371800 uncharacterized LOC105371800 XR_001756634.2 5.7% (many diffs)
69 human 135398 C6orf141 chromosome 6 open reading f... NR_146861.2 5.7% (many diffs)
70 human 105371800 LOC105371800 uncharacterized LOC105371800 XR_001756631.2 5.6% (many diffs)
71 human 105371800 LOC105371800 uncharacterized LOC105371800 XR_001756632.2 5.6% (many diffs)
72 human 105371800 LOC105371800 uncharacterized LOC105371800 XR_001756633.2 5.6% (many diffs)
73 human 105371800 LOC105371800 uncharacterized LOC105371800 XR_001756630.2 5.6% (many diffs)
74 human 105373328 LOC105373328 uncharacterized LOC105373328 XR_938572.3 5.5% (many diffs)
75 human 112268177 LOC112268177 uncharacterized LOC112268177 XR_002957914.1 5.4% (many diffs)
76 human 93349 SP140L SP140 nuclear body protein ... XR_002959365.1 5.4% (many diffs)
77 human 119504 ANAPC16 anaphase promoting complex ... NR_038391.2 5.4% (many diffs)
78 human 100874243 PRICKLE2-AS3 PRICKLE2 antisense RNA 3 NR_046702.1 5.3% (many diffs)
79 human 101927476 LOC101927476 uncharacterized LOC101927476 NR_110386.1 5.3% (many diffs)
80 human 10695 CNPY3 canopy FGF signaling regula... NR_134881.1 5.3% (many diffs)
81 human 119504 ANAPC16 anaphase promoting complex ... NR_038392.2 5.2% (many diffs)
82 human 8847 DLEU2 deleted in lymphocytic leuk... NR_152566.1 5.2% (many diffs)
83 human 128989 TANGO2 transport and golgi organiz... NR_104274.2 5.1% (many diffs)
84 human 112268131 LOC112268131 uncharacterized LOC112268131 XR_002957599.1 5.1% (many diffs)
85 human 9601 PDIA4 protein disulfide isomerase... NR_163905.1 5.1% (many diffs)
86 human 9753 ZSCAN12 zinc finger and SCAN domain... NR_160526.1 5% (many diffs)
87 human 9753 ZSCAN12 zinc finger and SCAN domain... NR_136511.2 5% (many diffs)
88 human 9129 PRPF3 pre-mRNA processing factor 3 XR_001737540.2 5% (many diffs)
89 human 6301 SARS1 seryl-tRNA synthetase 1 NR_034072.1 5% (many diffs)
90 human 101930307 LOC101930307 uncharacterized LOC101930307 XM_024447738.1 4.9% 3.4% (many diffs)
91 human 105376289 LOC105376289 uncharacterized LOC105376289 XR_930386.2 4.9% (many diffs)
92 human 57212 TP73-AS1 TP73 antisense RNA 1 NR_033712.1 4.9% (many diffs)
93 human 112268131 LOC112268131 uncharacterized LOC112268131 XR_002957600.1 4.9% (many diffs)
94 human 128989 TANGO2 transport and golgi organiz... NR_136206.1 4.8% (many diffs)
95 human 101930307 LOC101930307 uncharacterized LOC101930307 XM_017015425.1 4.8% 3.3% (many diffs)
96 human 128989 TANGO2 transport and golgi organiz... NR_104275.2 4.8% (many diffs)
97 human 128989 TANGO2 transport and golgi organiz... NR_136212.1 4.7% (many diffs)
98 human 112268131 LOC112268131 uncharacterized LOC112268131 XR_002957598.1 4.7% (many diffs)
99 human 107985485 LOC107985485 uncharacterized LOC107985485 XR_001755092.1 4.6% (many diffs)
100 human 128989 TANGO2 transport and golgi organiz... NR_136211.1 4.6% (many diffs)
101 human 112268131 LOC112268131 uncharacterized LOC112268131 XR_002957596.1 4.6% (many diffs)
102 human 84928 TMEM209 transmembrane protein 209 NR_156699.2 4.6% (many diffs)
103 human 112268131 LOC112268131 uncharacterized LOC112268131 XR_002957597.1 4.5% (many diffs)
104 human 105377375 LOC105377375 uncharacterized LOC105377375 XR_939087.2 4.4% (many diffs)
105 human 112267943 LOC112267943 uncharacterized LOC112267943 XR_002956245.1 4.3% (many diffs)
106 human 105377023 LOC105377023 uncharacterized LOC105377023 XR_940700.3 4.2% (many diffs)
107 human 55501 CHST12 carbohydrate sulfotransfera... XR_002956463.1 4.2% (many diffs)
108 human 105377921 LOC105377921 uncharacterized LOC105377921 XR_942832.2 4.2% (many diffs)
109 human 4750 NEK1 NIMA related kinase 1 NR_164631.1 4.2% (many diffs)
110 human 100289019 SLC25A25-AS1 SLC25A25 antisense RNA 1 NR_033374.1 4.2% (many diffs)
111 human 23639 LRRC6 leucine rich repeat contain... NR_135911.2 4.2% (many diffs)
112 human 50809 HP1BP3 heterochromatin protein 1 b... XR_001737207.1 4.1% (many diffs)
113 human 54581 SCAND2P SCAN domain containing 2 ps... NR_003654.2 4% (many diffs)
114 human 57212 TP73-AS1 TP73 antisense RNA 1 NR_033708.1 4% (many diffs)
115 human 100131564 CCDC18-AS1 CCDC18 antisense RNA 1 NR_034089.1 4% (many diffs)
116 human 23639 LRRC6 leucine rich repeat contain... NR_135913.2 3.9% (many diffs)
117 human 107984092 LOC107984092 uncharacterized LOC107984092 XR_002958934.1 3.7% (many diffs)
118 human 107984092 LOC107984092 uncharacterized LOC107984092 XR_002959009.1 3.7% (many diffs)
119 human 9753 ZSCAN12 zinc finger and SCAN domain... NR_028077.2 3.7% (many diffs)
120 human 347862 GATD1 glutamine amidotransferase ... NR_134867.2 3.6% (many diffs)
121 human 80336 PABPC1L poly(A) binding protein cyt... NR_134987.2 3.6% (many diffs)
122 human 27321 TNRC17 trinucleotide repeat contai... NR_146582.1 3.6% (many diffs)
123 human 23639 LRRC6 leucine rich repeat contain... NR_135912.2 3.6% (many diffs)
124 human 100506834 GAS1RR GAS1 adjacent regulatory RNA NR_049794.1 3.6% (many diffs)
125 human 105371800 LOC105371800 uncharacterized LOC105371800 XR_001756629.2 3.6% (many diffs)
126 human 105371800 LOC105371800 uncharacterized LOC105371800 XR_001752918.2 3.6% (many diffs)
127 human 105372249 LOC105372249 uncharacterized LOC105372249 XR_001753848.2 3.6% (many diffs)
128 human 347862 GATD1 glutamine amidotransferase ... NR_134868.2 3.6% (many diffs)
129 human 347862 GATD1 glutamine amidotransferase ... XR_242802.3 3.6% (many diffs)
130 human 100130027 LOC100130027 uncharacterized LOC100130027 NR_147501.1 3.6% (many diffs)
131 human 8837 CFLAR CASP8 and FADD like apoptos... XR_001739016.2 3.5% (many diffs)
132 human 54581 SCAND2P SCAN domain containing 2 ps... NR_004859.1 3.5% (many diffs)
133 human 9753 ZSCAN12 zinc finger and SCAN domain... NR_136509.2 3.5% (many diffs)
134 human 9753 ZSCAN12 zinc finger and SCAN domain... NR_136512.2 3.5% (many diffs)
135 human 105378589 LOC105378589 uncharacterized LOC105378589 XR_001737843.2 3.5% (many diffs)
136 human 50809 HP1BP3 heterochromatin protein 1 b... XR_001737206.2 3.5% (many diffs)
137 human 57212 TP73-AS1 TP73 antisense RNA 1 NR_033709.1 3.4% (many diffs)
138 human 135293 PM20D2 peptidase M20 domain contai... XR_001743167.1 3.4% (many diffs)
139 human 9753 ZSCAN12 zinc finger and SCAN domain... NR_136510.2 3.4% (many diffs)
140 human 105378589 LOC105378589 uncharacterized LOC105378589 XR_001737844.2 3.3% (many diffs)
141 human 7297 TYK2 tyrosine kinase 2 XR_001753751.1 3.3% (many diffs)
142 human 105371800 LOC105371800 uncharacterized LOC105371800 XR_001752916.2 3.3% (many diffs)
143 human 4808 NHLH2 nescient helix-loop-helix 2 XR_946659.3 3.3% (many diffs)
144 human 11184 MAP4K1 mitogen-activated protein k... NM_007181.6 3.2% 1.9% (many diffs)
145 human 79876 UBA5 ubiquitin like modifier act... XR_001740272.1 3.2% (many diffs)
146 human 107986584 LOC107986584 uncharacterized LOC107986584 XR_001744071.1 3.2% (many diffs)
147 human 11184 MAP4K1 mitogen-activated protein k... XM_017026231.1 3.1% 1.8% (many diffs)
148 human 11184 MAP4K1 mitogen-activated protein k... XM_011526403.1 3.1% 1.8% (many diffs)
149 human 107986871 LOC107986871 ubiquitin-conjugating enzym... XR_002956669.1 2.9% (many diffs)
150 human 9392 TGFBRAP1 transforming growth factor ... NR_137328.1 2.8% (many diffs)
151 human 51182 HSPA14 heat shock protein family A... NR_103464.2 2.8% (many diffs)
152 human 57212 TP73-AS1 TP73 antisense RNA 1 NR_033710.1 2.7% (many diffs)
153 human 29063 ZCCHC4 zinc finger CCHC-type conta... XR_925324.3 2.7% (many diffs)
154 human 84284 NTPCR nucleoside-triphosphatase, ... NR_138028.2 2.7% (many diffs)
155 human 84284 NTPCR nucleoside-triphosphatase, ... NR_138025.2 2.6% (many diffs)
156 human 94056 SYAP1 synapse associated protein 1 NR_033181.2 2.6% (many diffs)
157 human 54896 SLC66A1 solute carrier family 66 me... XR_001737254.1 2.6% (many diffs)
158 human 4771 NF2 neurofibromin 2 NR_156186.2 2.6% (many diffs)
159 human 84284 NTPCR nucleoside-triphosphatase, ... NR_138026.2 2.6% (many diffs)
160 human 84284 NTPCR nucleoside-triphosphatase, ... NR_138024.2 2.6% (many diffs)
161 human 8739 HRK harakiri, BCL2 interacting ... NR_073189.3 2.6% (many diffs)
162 human 26505 CNNM3 cyclin and CBS domain dival... XR_001738702.2 2.6% (many diffs)
163 human 63967 CLSPN claspin XR_001737361.2 2.5% (many diffs)
164 human 57212 TP73-AS1 TP73 antisense RNA 1 NR_033711.1 2.5% (many diffs)
165 human 23241 PACS2 phosphofurin acidic cluster... XR_001750200.2 2.5% (many diffs)
166 human 3066 HDAC2 histone deacetylase 2 NR_073443.1 2.4% (many diffs)
167 human 6867 TACC1 transforming acidic coiled-... NR_148048.2 2.2% (many diffs)
168 human 6867 TACC1 transforming acidic coiled-... NR_148047.2 2.2% (many diffs)
169 human 6867 TACC1 transforming acidic coiled-... NR_148053.2 2.2% (many diffs)
170 human 105372579 LOC105372579 uncharacterized LOC105372579 XR_001754564.2 2.2% (many diffs)
171 human 8837 CFLAR CASP8 and FADD like apoptos... NR_147242.2 2.1% (many diffs)
172 human 331 XIAP X-linked inhibitor of apopt... NR_037916.1 2.1% (many diffs)
173 human 80975 TMPRSS5 transmembrane serine protea... XR_001747992.1 2% (many diffs)
174 human 80975 TMPRSS5 transmembrane serine protea... XR_001747991.1 2% (many diffs)
175 human 6867 TACC1 transforming acidic coiled-... NR_148052.2 2% (many diffs)
176 human 80975 TMPRSS5 transmembrane serine protea... XR_001747990.1 2% (many diffs)
177 human 653857 ACTR3C actin related protein 3C NR_147022.1 2% (many diffs)
178 human 653857 ACTR3C actin related protein 3C NR_147021.1 2% (many diffs)
179 human 653857 ACTR3C actin related protein 3C NR_147020.1 2% (many diffs)
180 human 114004397 CA5BP1-CA5B CA5BP1-CA5B readthrough NR_160544.1 1.9% (many diffs)
181 human 653857 ACTR3C actin related protein 3C NR_147016.1 1.9% (many diffs)
182 human 653857 ACTR3C actin related protein 3C NR_147014.1 1.9% (many diffs)
183 human 653857 ACTR3C actin related protein 3C NR_147018.1 1.9% (many diffs)
184 human 653857 ACTR3C actin related protein 3C NR_147015.1 1.9% (many diffs)
185 human 653857 ACTR3C actin related protein 3C NR_147017.1 1.9% (many diffs)
186 human 105375634 LOC105375634 uncharacterized LOC105375634 XR_928392.3 1.8% (many diffs)
187 human 55346 TCP11L1 t-complex 11 like 1 XR_001747920.2 1.8% (many diffs)
188 human 653857 ACTR3C actin related protein 3C NR_147019.1 1.8% (many diffs)
189 human 105379504 LOC105379504 uncharacterized LOC105379504 XR_001754936.1 1.8% (many diffs)
190 human 105372793 LOC105372793 uncharacterized LOC105372793 XR_001755014.1 1.8% (many diffs)
191 human 653857 ACTR3C actin related protein 3C NR_147012.2 1.8% (many diffs)
192 human 55131 RBM28 RNA binding motif protein 28 XR_001744830.1 1.8% (many diffs)
193 human 526 ATP6V1B2 ATPase H+ transporting V1 s... XR_002956632.1 1.7% (many diffs)
194 human 526 ATP6V1B2 ATPase H+ transporting V1 s... XR_002956633.1 1.7% (many diffs)
195 human 3066 HDAC2 histone deacetylase 2 NR_033441.2 1.6% (many diffs)
196 human 112267989 LOC112267989 uncharacterized LOC112267989 XR_002956527.1 1.6% (many diffs)
197 human 112267989 LOC112267989 uncharacterized LOC112267989 XR_002959037.1 1.6% (many diffs)
198 human 56886 UGGT1 UDP-glucose glycoprotein gl... XR_922969.3 1.5% (many diffs)
199 human 9819 TSC22D2 TSC22 domain family member 2 NR_130136.2 1.4% (many diffs)
200 human 56886 UGGT1 UDP-glucose glycoprotein gl... NR_027671.3 1.4% (many diffs)
201 human 57506 MAVS mitochondrial antiviral sig... NR_037921.2 1.3% (many diffs)
202 human 58155 PTBP2 polypyrimidine tract bindin... NR_125357.2 1.3% (many diffs)
203 human 105375079 LOC105375079 uncharacterized LOC105375079 XR_001743802.2 1.3% (many diffs)
204 human 105375079 LOC105375079 uncharacterized LOC105375079 XR_001743801.2 .8% (many diffs)
205 human 100131257 LOC100131257 zinc finger protein 655 pse... NR_034022.1 .7% (many diffs)
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Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
66
ORF end:
252
ORF length:
186
Sequence:
1gttcgttgca acaaattgat gagcaatgct tttttataat gccaactttg tacaaaaaag
61ttggcatgca gtggcgccat ctcagctcac tgcaacctcc atctcccagg ttcaagcgat
121tctcgtgcct cggcctcctg agtagctggg attacaggcg tgtgccacta cactcaacta
181atttttgtat ttttaggaga gacggggttt caccctgttg gccaggctgg tctcgaactc
241ctgacctcaa gtnnccaact ttcttgtaca aagttggcat tataagaaag cattgcttat
301caatttgttg caacgaac

Download FASTA (ORF) (Full)