pEpi35 program activity among Epithelial cells

Activation of pEpi35
pEpi35 gene program activation in Epithelial cells. (Top) Activation of program across all epithelial cells. (Bottom) Activation in MMRd (left), MMRp (middle), and normal epithelial cells (right).

Epithelial cell compositional overview

Overview of epithelial cell composition
Overview of epithelial cell composition. A tSNE plot colored by: (left) MMR status of epithelial cells, (middle) specimen, (righ) epithelial cell sub-type.
Gene program activation of pEpi35 by different cell subtypes
pEpi35 gene program activation among cells of different subtypes (Epithelial cells).

pEpi35 top program genes

Top genes of pEpi35 program
Bar plots showing the top genes for each gene program, ranked by (left) unique weights, (right) raw weights.

pEpi35 gene-set enrichment of top program genes (using g-profiler)

GSEA wth gene profiler of pEpi35
pEpi35 gene-set enrichment summary using g-profiler webtool.
Source termID Name Padj Tsize Qsize Overlap Precision Recall
GO:BP GO:0006816 calcium ion transport 0.00155097047163632 436 42 8 0.19047619047619 0.018348623853211
GO:BP GO:0006812 cation transport 0.00155097047163632 1197 46 13 0.282608695652174 0.0108604845446951
GO:BP GO:0015698 inorganic anion transport 0.00155097047163632 169 46 6 0.130434782608696 0.0355029585798817
GO:BP GO:0044057 regulation of system process 0.00155097047163632 623 49 10 0.204081632653061 0.0160513643659711
GO:BP GO:0003008 system process 0.00155097047163632 2293 49 19 0.387755102040816 0.00828608809419974
GO:BP GO:0034220 ion transmembrane transport 0.00155097047163632 1397 46 14 0.304347826086957 0.0100214745884037
GO:BP GO:0006821 chloride transport 0.00177177410072372 109 46 5 0.108695652173913 0.0458715596330275
GO:BP GO:0098660 inorganic ion transmembrane transport 0.00184221366216056 883 46 11 0.239130434782609 0.0124575311438279
GO:BP GO:0030001 metal ion transport 0.00341432472254844 514 42 8 0.19047619047619 0.0155642023346304
GO:BP GO:0050926 regulation of positive chemotaxis 0.00405617844430549 27 42 3 0.0714285714285714 0.111111111111111
GO:BP GO:0055085 transmembrane transport 0.00405617844430549 1635 46 14 0.304347826086957 0.00856269113149847
GO:BP GO:0050927 positive regulation of positive chemotaxis 0.00405617844430549 26 42 3 0.0714285714285714 0.115384615384615
GO:BP GO:0098656 anion transmembrane transport 0.00619353132247971 550 46 8 0.173913043478261 0.0145454545454545
GO:BP GO:0003013 circulatory system process 0.00701389570956837 637 42 8 0.19047619047619 0.0125588697017268
GO:BP GO:0090279 regulation of calcium ion import 0.00977816068589924 43 42 3 0.0714285714285714 0.0697674418604651
GO:BP GO:0050920 regulation of chemotaxis 0.010342153854942 226 42 5 0.119047619047619 0.0221238938053097
GO:BP GO:0015701 bicarbonate transport 0.010342153854942 42 46 3 0.0652173913043478 0.0714285714285714
GO:BP GO:0006936 muscle contraction 0.010342153854942 364 41 6 0.146341463414634 0.0164835164835165
GO:BP GO:0008015 blood circulation 0.0138674245011871 553 42 7 0.166666666666667 0.0126582278481013
GO:BP GO:0050921 positive regulation of chemotaxis 0.015675884335652 141 42 4 0.0952380952380952 0.0283687943262411
GO:BP GO:0051924 regulation of calcium ion transport 0.015675884335652 259 42 5 0.119047619047619 0.0193050193050193
GO:BP GO:0071675 regulation of mononuclear cell migration 0.0158708072191678 113 53 4 0.0754716981132075 0.0353982300884956
GO:BP GO:0010959 regulation of metal ion transport 0.0162412276780519 268 42 5 0.119047619047619 0.0186567164179104
GO:BP GO:0032879 regulation of localization 0.0172760248170221 2872 42 16 0.380952380952381 0.00557103064066852
GO:BP GO:1903522 regulation of blood circulation 0.0195358295440492 301 40 5 0.125 0.0166112956810631
GO:BP GO:0051179 localization 0.0195358295440492 6901 42 27 0.642857142857143 0.00391247645268802
GO:BP GO:0050918 positive chemotaxis 0.0195358295440492 68 42 3 0.0714285714285714 0.0441176470588235
GO:BP GO:0098662 inorganic cation transmembrane transport 0.020126724682546 794 34 7 0.205882352941176 0.00881612090680101
GO:BP GO:0006937 regulation of muscle contraction 0.020126724682546 170 41 4 0.0975609756097561 0.0235294117647059
GO:BP GO:0003012 muscle system process 0.0211506221823958 471 41 6 0.146341463414634 0.0127388535031847
GO:BP GO:0042330 taxis 0.0216515007430146 656 42 7 0.166666666666667 0.0106707317073171
GO:BP GO:0034765 regulation of ion transmembrane transport 0.0216515007430146 583 34 6 0.176470588235294 0.0102915951972556
GO:BP GO:0034762 regulation of transmembrane transport 0.0216515007430146 588 34 6 0.176470588235294 0.0102040816326531
GO:BP GO:0006935 chemotaxis 0.0216515007430146 653 42 7 0.166666666666667 0.0107197549770291
GO:BP GO:2000401 regulation of lymphocyte migration 0.0244332130698698 63 53 3 0.0566037735849057 0.0476190476190476
GO:BP GO:0050801 ion homeostasis 0.0252855569337246 808 46 8 0.173913043478261 0.0099009900990099
GO:BP GO:0035725 sodium ion transmembrane transport 0.026043571995168 171 46 4 0.0869565217391304 0.0233918128654971
GO:BP GO:0043269 regulation of ion transport 0.0272119300570335 1379 42 10 0.238095238095238 0.00725163161711385
GO:BP GO:0070509 calcium ion import 0.027876463502601 86 42 3 0.0714285714285714 0.0348837209302326
GO:BP GO:0016477 cell migration 0.027876463502601 1649 42 11 0.261904761904762 0.00667070952092177
GO:BP GO:0050807 regulation of synapse organization 0.0295964023980266 212 19 3 0.157894736842105 0.0141509433962264
GO:BP GO:0098655 cation transmembrane transport 0.0295964023980266 914 34 7 0.205882352941176 0.00765864332603939
GO:BP GO:1902476 chloride transmembrane transport 0.0295964023980266 95 40 3 0.075 0.0315789473684211
GO:BP GO:0007626 locomotory behavior 0.0295964023980266 189 46 4 0.0869565217391304 0.0211640211640212
GO:BP GO:0008016 regulation of heart contraction 0.0295964023980266 254 34 4 0.117647058823529 0.015748031496063
GO:BP GO:0070588 calcium ion transmembrane transport 0.0306238343659416 320 28 4 0.142857142857143 0.0125
GO:BP GO:0030003 cellular cation homeostasis 0.0309414690257801 673 46 7 0.152173913043478 0.0104011887072808
GO:BP GO:0050803 regulation of synapse structure or activity 0.0323454166555834 223 19 3 0.157894736842105 0.0134529147982063
GO:BP GO:0040011 locomotion 0.0331427447942783 2028 42 12 0.285714285714286 0.00591715976331361
GO:BP GO:0006873 cellular ion homeostasis 0.0331427447942783 687 46 7 0.152173913043478 0.0101892285298399
GO:BP GO:0006810 transport 0.0375884838683117 5224 46 23 0.5 0.00440275650842266
GO:BP GO:0006939 smooth muscle contraction 0.0375884838683117 110 41 3 0.0731707317073171 0.0272727272727273
GO:BP GO:0060047 heart contraction 0.0376778238841827 288 34 4 0.117647058823529 0.0138888888888889
GO:BP GO:0048870 cell motility 0.0398406238934338 1828 42 11 0.261904761904762 0.00601750547045952
GO:BP GO:0098661 inorganic anion transmembrane transport 0.0398406238934338 118 40 3 0.075 0.0254237288135593
GO:BP GO:0071674 mononuclear cell migration 0.0398406238934338 191 53 4 0.0754716981132075 0.0209424083769634
GO:BP GO:0003015 heart process 0.0398406238934338 298 34 4 0.117647058823529 0.0134228187919463
GO:BP GO:0051674 localization of cell 0.0398406238934338 1828 42 11 0.261904761904762 0.00601750547045952
GO:BP GO:0051049 regulation of transport 0.0398406238934338 1809 42 11 0.261904761904762 0.00608070757324489
GO:BP GO:0055080 cation homeostasis 0.0418968386640017 750 46 7 0.152173913043478 0.00933333333333333
GO:BP GO:0090257 regulation of muscle system process 0.0418968386640017 257 41 4 0.0975609756097561 0.0155642023346304
GO:BP GO:0061337 cardiac conduction 0.0422555441140425 150 34 3 0.0882352941176471 0.02
GO:BP GO:0051234 establishment of localization 0.0422555441140425 5372 46 23 0.5 0.00428145941921072
GO:BP GO:0003014 renal system process 0.0422555441140425 126 41 3 0.0731707317073171 0.0238095238095238
GO:BP GO:0098771 inorganic ion homeostasis 0.0422555441140425 761 46 7 0.152173913043478 0.00919842312746386
GO:BP GO:0006814 sodium ion transport 0.0432232025352228 241 46 4 0.0869565217391304 0.016597510373444
GO:BP GO:0002685 regulation of leukocyte migration 0.0435211429811587 211 53 4 0.0754716981132075 0.018957345971564
GO:BP GO:0030534 adult behavior 0.0438566197560389 131 42 3 0.0714285714285714 0.0229007633587786
GO:BP GO:0040012 regulation of locomotion 0.0438566197560389 1104 42 8 0.19047619047619 0.0072463768115942
GO:BP GO:0006959 humoral immune response 0.0438566197560389 384 30 4 0.133333333333333 0.0104166666666667
GO:BP GO:0050900 leukocyte migration 0.0447531393908989 511 53 6 0.113207547169811 0.0117416829745597
GO:BP GO:0051270 regulation of cellular component movement 0.0486522342866267 1141 42 8 0.19047619047619 0.007011393514461
GO:CC GO:0071944 cell periphery 0.00101246629368815 6169 50 32 0.64 0.00518722645485492
GO:CC GO:0005886 plasma membrane 0.00103257624629024 5681 50 30 0.6 0.00528076042950185
GO:CC GO:0016020 membrane 0.0379437556482839 9838 50 37 0.74 0.00376092701768652
GO:CC GO:0005615 extracellular space 0.0379437556482839 3594 4 4 1 0.00111296605453534
GO:MF GO:0015103 inorganic anion transmembrane transporter activity 0.0122234227643947 142 15 3 0.2 0.0211267605633803
GO:MF GO:0015318 inorganic molecular entity transmembrane transporter activity 0.0122234227643947 831 46 9 0.195652173913043 0.0108303249097473
GO:MF GO:0005216 ion channel activity 0.0176261990400587 435 41 6 0.146341463414634 0.0137931034482759
GO:MF GO:0015075 ion transmembrane transporter activity 0.0176261990400587 956 46 9 0.195652173913043 0.00941422594142259
GO:MF GO:0022803 passive transmembrane transporter activity 0.0183371600550699 485 41 6 0.146341463414634 0.0123711340206186
GO:MF GO:0015267 channel activity 0.0183371600550699 484 41 6 0.146341463414634 0.012396694214876
GO:MF GO:0008509 anion transmembrane transporter activity 0.020441211946784 464 46 6 0.130434782608696 0.0129310344827586
GO:MF GO:0022857 transmembrane transporter activity 0.0209069149853661 1075 46 9 0.195652173913043 0.00837209302325581
GO:MF GO:0005215 transporter activity 0.033162714781272 1185 46 9 0.195652173913043 0.00759493670886076
GO:MF GO:0022890 inorganic cation transmembrane transporter activity 0.0379464189648347 591 46 6 0.130434782608696 0.0101522842639594
GO:MF GO:0044325 ion channel binding 0.0379464189648347 135 40 3 0.075 0.0222222222222222
GO:MF GO:0008324 cation transmembrane transporter activity 0.0457735384010176 639 46 6 0.130434782608696 0.00938967136150235
GO:MF GO:0046873 metal ion transmembrane transporter activity 0.0457735384010176 435 46 5 0.108695652173913 0.0114942528735632
GO:MF GO:0005261 cation channel activity 0.0463292343699831 340 41 4 0.0975609756097561 0.0117647058823529
GO:MF GO:0015081 sodium ion transmembrane transporter activity 0.0463292343699831 151 46 3 0.0652173913043478 0.0198675496688742
GO:MF GO:0015293 symporter activity 0.0463292343699831 143 46 3 0.0652173913043478 0.020979020979021
GO:MF GO:0022836 gated channel activity 0.0463292343699831 341 41 4 0.0975609756097561 0.0117302052785924

pEpi35 transcription factor target enrichment of top program genes

TF enrichment of pEpi35
Possible regulators of activation of pEpi35 in epithelial cells. (Left) pearson correlation of TF expression and gene expression program activatity in Epithelial cells, (right) negative log p-value for enrichment of TF binding sites among top uniqueness weighted genes for the gene program. Blue bars indicate significant TFs meeting the following criteria: FDR<0.1, overlap > 2, Corr. R > 0, permutation based p-value < 0.05.