pEpiTd21 program activity among Epithelial cells

Activation of pEpiTd21
pEpiTd21 gene program activation in Epithelial cells. (Top) Activation of program across all epithelial cells. (Bottom) Activation in MMRd (left), MMRp (middle), and normal epithelial cells (right).

Epithelial cell compositional overview

Overview of epithelial cell composition
Overview of epithelial cell composition. A tSNE plot colored by: (left) MMR status of epithelial cells, (middle) specimen, (righ) epithelial cell sub-type.
Gene program activation of pEpiTd21 by different cell subtypes
pEpiTd21 gene program activation among cells of different subtypes (Epithelial cells).

pEpiTd21 top program genes

Top genes of pEpiTd21 program
Bar plots showing the top genes for each gene program, ranked by (left) unique weights, (right) raw weights.

pEpiTd21 gene-set enrichment of top program genes (using g-profiler)

GSEA wth gene profiler of pEpiTd21
pEpiTd21 gene-set enrichment summary using g-profiler webtool.
Source termID Name Padj Tsize Qsize Overlap Precision Recall
GO:BP GO:0030277 maintenance of gastrointestinal epithelium 2.69781490716241e-05 21 5 3 0.6 0.142857142857143
GO:BP GO:0010669 epithelial structure maintenance 3.70348933052736e-05 29 5 3 0.6 0.103448275862069
GO:BP GO:0001894 tissue homeostasis 0.000474364187074315 270 6 4 0.666666666666667 0.0148148148148148
GO:BP GO:0022600 digestive system process 0.000864870549035123 102 5 3 0.6 0.0294117647058824
GO:BP GO:0007586 digestion 0.00183684471533182 141 5 3 0.6 0.0212765957446809
GO:BP GO:0060249 anatomical structure homeostasis 0.00238685166337595 482 6 4 0.666666666666667 0.00829875518672199
GO:BP GO:0016266 O-glycan processing 0.00252084380093978 58 41 4 0.0975609756097561 0.0689655172413793
GO:BP GO:0048871 multicellular organismal homeostasis 0.00260863008418592 530 6 4 0.666666666666667 0.00754716981132075
GO:BP GO:0006493 protein O-linked glycosylation 0.0182770982051589 102 41 4 0.0975609756097561 0.0392156862745098
GO:CC GO:0005576 extracellular region 1.41581861051962e-05 4567 40 26 0.65 0.00569301510838625
GO:CC GO:0005615 extracellular space 1.59147968561526e-05 3594 18 14 0.777777777777778 0.00389538119087368
GO:CC GO:0043230 extracellular organelle 5.03314764995808e-05 2263 21 12 0.571428571428571 0.00530269553689792
GO:CC GO:1903561 extracellular vesicle 5.03314764995808e-05 2261 21 12 0.571428571428571 0.00530738611233967
GO:CC GO:0070062 extracellular exosome 5.03314764995808e-05 2176 21 12 0.571428571428571 0.00551470588235294
GO:CC GO:0019814 immunoglobulin complex 0.00186814240471218 167 104 7 0.0673076923076923 0.0419161676646707
GO:CC GO:0031982 vesicle 0.0110402417863043 4055 18 11 0.611111111111111 0.00271270036991369
GO:CC GO:0005902 microvillus 0.0110402417863043 91 66 4 0.0606060606060606 0.043956043956044
GO:MF GO:0004896 cytokine receptor activity 0.0341736875732192 99 92 4 0.0434782608695652 0.0404040404040404
GO:MF GO:0016491 oxidoreductase activity 0.0341736875732192 771 22 5 0.227272727272727 0.00648508430609598
GO:MF GO:0030246 carbohydrate binding 0.0341736875732192 275 33 4 0.121212121212121 0.0145454545454545
GO:MF GO:0008146 sulfotransferase activity 0.0414118337656584 56 93 3 0.032258064516129 0.0535714285714286
GO:MF GO:0140375 immune receptor activity 0.0440753413860695 133 92 4 0.0434782608695652 0.0300751879699248
GO:MF GO:0016782 transferase activity, transferring sulfur-containing groups 0.0487359299212589 74 93 3 0.032258064516129 0.0405405405405405
GO:MF GO:0005102 signaling receptor binding 0.0487359299212589 1672 5 3 0.6 0.00179425837320574
REAC REAC:R-HSA-913709 O-linked glycosylation of mucins 0.00518531060405489 61 41 4 0.0975609756097561 0.0655737704918033
REAC REAC:R-HSA-5173105 O-linked glycosylation 0.0225371390404893 107 41 4 0.0975609756097561 0.0373831775700935
WP WP:WP2291 Deregulation of Rab and Rab Effector Genes in Bladder Cancer 0.0046475195804322 16 37 3 0.0810810810810811 0.1875

pEpiTd21 transcription factor target enrichment of top program genes

TF enrichment of pEpiTd21
Possible regulators of activation of pEpiTd21 in epithelial cells. (Left) pearson correlation of TF expression and gene expression program activatity in Epithelial cells, (right) negative log p-value for enrichment of TF binding sites among top uniqueness weighted genes for the gene program. Blue bars indicate significant TFs meeting the following criteria: FDR<0.1, overlap > 2, Corr. R > 0, permutation based p-value < 0.05.