pEpiTd15 program activity among Epithelial cells

Activation of pEpiTd15
pEpiTd15 gene program activation in Epithelial cells. (Top) Activation of program across all epithelial cells. (Bottom) Activation in MMRd (left), MMRp (middle), and normal epithelial cells (right).

Epithelial cell compositional overview

Overview of epithelial cell composition
Overview of epithelial cell composition. A tSNE plot colored by: (left) MMR status of epithelial cells, (middle) specimen, (righ) epithelial cell sub-type.
Gene program activation of pEpiTd15 by different cell subtypes
pEpiTd15 gene program activation among cells of different subtypes (Epithelial cells).

pEpiTd15 top program genes

Top genes of pEpiTd15 program
Bar plots showing the top genes for each gene program, ranked by (left) unique weights, (right) raw weights.

pEpiTd15 gene-set enrichment of top program genes (using g-profiler)

GSEA wth gene profiler of pEpiTd15
pEpiTd15 gene-set enrichment summary using g-profiler webtool.
Source termID Name Padj Tsize Qsize Overlap Precision Recall
GO:CC GO:0043195 terminal bouton 0.00308112995446502 57 57 4 0.0701754385964912 0.0701754385964912
GO:CC GO:0005615 extracellular space 0.00308112995446502 3594 76 30 0.394736842105263 0.00834724540901502
GO:CC GO:0005576 extracellular region 0.00308112995446502 4567 73 34 0.465753424657534 0.00744471206481279
GO:CC GO:0099503 secretory vesicle 0.0153866845077745 1020 57 11 0.192982456140351 0.0107843137254902
GO:CC GO:0012505 endomembrane system 0.0153866845077745 4640 113 45 0.398230088495575 0.00969827586206897
GO:CC GO:0030141 secretory granule 0.0162288003798527 851 49 9 0.183673469387755 0.0105757931844888
GO:CC GO:0016528 sarcoplasm 0.0179225598244493 79 88 4 0.0454545454545455 0.0506329113924051
GO:CC GO:0030054 cell junction 0.0179225598244493 2105 70 18 0.257142857142857 0.00855106888361045
GO:CC GO:0071944 cell periphery 0.0179225598244493 6169 71 37 0.52112676056338 0.005997730588426
GO:CC GO:0043679 axon terminus 0.0184876544418409 127 57 4 0.0701754385964912 0.031496062992126
GO:CC GO:0070382 exocytic vesicle 0.0184876544418409 229 57 5 0.087719298245614 0.0218340611353712
GO:CC GO:0031982 vesicle 0.021684075753874 4055 70 27 0.385714285714286 0.00665844636251541
GO:CC GO:0044306 neuron projection terminus 0.022389956606804 144 57 4 0.0701754385964912 0.0277777777777778
GO:CC GO:0070062 extracellular exosome 0.0250499338758002 2176 61 16 0.262295081967213 0.00735294117647059
GO:CC GO:0062023 collagen-containing extracellular matrix 0.0320034353230836 421 121 9 0.0743801652892562 0.0213776722090261
GO:CC GO:0043230 extracellular organelle 0.0320034353230836 2263 61 16 0.262295081967213 0.0070702607158639
GO:CC GO:1903561 extracellular vesicle 0.0320034353230836 2261 61 16 0.262295081967213 0.0070765148164529
GO:CC GO:0098793 presynapse 0.0327316000993562 521 64 7 0.109375 0.0134357005758157
GO:CC GO:0030133 transport vesicle 0.0364265973685813 414 105 8 0.0761904761904762 0.0193236714975845
GO:CC GO:0005886 plasma membrane 0.0382838347606116 5681 71 33 0.464788732394366 0.00580883647245203
GO:CC GO:0097708 intracellular vesicle 0.0382838347606116 2428 70 18 0.257142857142857 0.00741350906095552
GO:CC GO:0031410 cytoplasmic vesicle 0.0382838347606116 2424 70 18 0.257142857142857 0.00742574257425743
GO:CC GO:0030312 external encapsulating structure 0.0451454204255146 563 121 10 0.0826446280991736 0.0177619893428064
GO:CC GO:0031012 extracellular matrix 0.0451454204255146 562 121 10 0.0826446280991736 0.0177935943060498
GO:CC GO:0008021 synaptic vesicle 0.0477422001124378 209 57 4 0.0701754385964912 0.0191387559808612
GO:CC GO:0150034 distal axon 0.0489536873301801 308 64 5 0.078125 0.0162337662337662
GO:CC GO:0030672 synaptic vesicle membrane 0.0494078411510804 111 57 3 0.0526315789473684 0.027027027027027
GO:CC GO:0099501 exocytic vesicle membrane 0.0494078411510804 111 57 3 0.0526315789473684 0.027027027027027
GO:CC GO:0016529 sarcoplasmic reticulum 0.0494078411510804 70 88 3 0.0340909090909091 0.0428571428571429
GO:MF GO:0005229 intracellular calcium activated chloride channel activity 0.00264490584721354 16 52 3 0.0576923076923077 0.1875
GO:MF GO:0061778 intracellular chloride channel activity 0.00264490584721354 16 52 3 0.0576923076923077 0.1875
GO:MF GO:0022839 ion gated channel activity 0.00748752798770591 43 87 4 0.0459770114942529 0.0930232558139535
GO:MF GO:0008509 anion transmembrane transporter activity 0.0157398310820222 464 127 11 0.0866141732283465 0.0237068965517241
GO:MF GO:0015108 chloride transmembrane transporter activity 0.0157398310820222 99 57 4 0.0701754385964912 0.0404040404040404
GO:MF GO:0004713 protein tyrosine kinase activity 0.0239916541388995 144 96 5 0.0520833333333333 0.0347222222222222
GO:MF GO:0004252 serine-type endopeptidase activity 0.0239916541388995 171 123 6 0.0487804878048781 0.0350877192982456
GO:MF GO:0015175 neutral amino acid transmembrane transporter activity 0.0239916541388995 34 102 3 0.0294117647058824 0.0882352941176471
GO:MF GO:0015103 inorganic anion transmembrane transporter activity 0.0239916541388995 142 57 4 0.0701754385964912 0.028169014084507
GO:MF GO:0015026 coreceptor activity 0.0239916541388995 47 68 3 0.0441176470588235 0.0638297872340425
GO:MF GO:0005509 calcium ion binding 0.0239916541388995 721 128 13 0.1015625 0.0180305131761442
GO:MF GO:0005254 chloride channel activity 0.0239916541388995 73 52 3 0.0576923076923077 0.0410958904109589
GO:MF GO:0005253 anion channel activity 0.025407507891247 85 52 3 0.0576923076923077 0.0352941176470588
GO:MF GO:0008236 serine-type peptidase activity 0.025407507891247 189 123 6 0.0487804878048781 0.0317460317460317
GO:MF GO:0015318 inorganic molecular entity transmembrane transporter activity 0.025407507891247 831 127 14 0.110236220472441 0.0168471720818291
GO:MF GO:0017171 serine hydrolase activity 0.025407507891247 193 123 6 0.0487804878048781 0.0310880829015544
GO:MF GO:0022857 transmembrane transporter activity 0.0265591863285892 1075 102 14 0.137254901960784 0.0130232558139535
GO:MF GO:0015267 channel activity 0.0265591863285892 484 52 6 0.115384615384615 0.012396694214876
GO:MF GO:0022803 passive transmembrane transporter activity 0.0265591863285892 485 52 6 0.115384615384615 0.0123711340206186
GO:MF GO:0015075 ion transmembrane transporter activity 0.0265591863285892 956 102 13 0.127450980392157 0.0135983263598326
GO:MF GO:0140096 catalytic activity, acting on a protein 0.0265591863285892 2332 71 18 0.253521126760563 0.00771869639794168
GO:MF GO:0001784 phosphotyrosine residue binding 0.0277108071912661 44 114 3 0.0263157894736842 0.0681818181818182
GO:MF GO:0022836 gated channel activity 0.0280701371755331 341 52 5 0.0961538461538462 0.0146627565982405
GO:MF GO:0042887 amide transmembrane transporter activity 0.029830147870947 58 90 3 0.0333333333333333 0.0517241379310345
GO:MF GO:0015179 L-amino acid transmembrane transporter activity 0.0341433057056373 56 102 3 0.0294117647058824 0.0535714285714286
GO:MF GO:0004175 endopeptidase activity 0.0349174103406732 450 63 6 0.0952380952380952 0.0133333333333333
GO:MF GO:0045309 protein phosphorylated amino acid binding 0.0381340935320641 56 114 3 0.0263157894736842 0.0535714285714286
GO:MF GO:1901618 organic hydroxy compound transmembrane transporter activity 0.0381340935320641 50 127 3 0.0236220472440945 0.06
GO:MF GO:0005215 transporter activity 0.0385771505750855 1185 102 14 0.137254901960784 0.0118143459915612
GO:MF GO:0005201 extracellular matrix structural constituent 0.038826536346787 171 121 5 0.0413223140495868 0.0292397660818713
GO:MF GO:0003713 transcription coactivator activity 0.0395237164026719 280 47 4 0.0851063829787234 0.0142857142857143
GO:MF GO:0008233 peptidase activity 0.0397152188564532 646 63 7 0.111111111111111 0.0108359133126935
GO:MF GO:0005216 ion channel activity 0.044394332989933 435 52 5 0.0961538461538462 0.0114942528735632
GO:MF GO:0098772 molecular function regulator 0.0452144053462171 2363 24 8 0.333333333333333 0.00338552687261955
GO:MF GO:0019838 growth factor binding 0.0487390304748893 138 107 4 0.0373831775700935 0.0289855072463768
GO:MF GO:0019199 transmembrane receptor protein kinase activity 0.0496830833541279 82 96 3 0.03125 0.0365853658536585
GO:MF GO:0015171 amino acid transmembrane transporter activity 0.0496830833541279 78 102 3 0.0294117647058824 0.0384615384615385
REAC REAC:R-HSA-2672351 Stimuli-sensing channels 0.0271568490940423 105 71 6 0.0845070422535211 0.0571428571428571
TF TF:M00176 Factor: AP-4; motif: CWCAGCTGGN 0.0429909572628257 2528 70 21 0.3 0.00830696202531645
TF TF:M10097_1 Factor: AP-4; motif: YCAGCTGNKN; match class: 1 0.0429909572628257 1554 62 15 0.241935483870968 0.00965250965250965
TF TF:M04797 Factor: Egr-1; motif: CCGCCCMCG 0.0429909572628257 5116 120 50 0.416666666666667 0.00977326035965598
TF TF:M12096_1 Factor: ZNF704; motif: NRCCGGCCGGYN; match class: 1 0.0429909572628257 80 111 5 0.045045045045045 0.0625
TF TF:M12096 Factor: ZNF704; motif: NRCCGGCCGGYN 0.0429909572628257 80 111 5 0.045045045045045 0.0625
TF TF:M09727_1 Factor: GEMIN3; motif: NCWGGRARRGRGNGNG; match class: 1 0.0429909572628257 3210 128 38 0.296875 0.0118380062305296
TF TF:M06089 Factor: ZNF26; motif: NAMTCAAGSGTA 0.0429909572628257 30 61 3 0.0491803278688525 0.1
TF TF:M11728 Factor: NF-1C; motif: NTTGGCNNNNTGCCARN 0.0429909572628257 2634 16 9 0.5625 0.00341685649202733
TF TF:M04152 Factor: TFAP2C; motif: NGCCCNNRGGCA 0.0429909572628257 7738 128 74 0.578125 0.00956319462393383
TF TF:M09973_1 Factor: CPBP; motif: GNNRGGGHGGGGNNGGGRN; match class: 1 0.0429909572628257 6607 129 64 0.496124031007752 0.00968669592856062
TF TF:M04811_1 Factor: RelA-p65; motif: BCWGGGRANNK; match class: 1 0.0429909572628257 1834 10 6 0.6 0.00327153762268266
TF TF:M01196_1 Factor: CTF1; motif: TGGCASCNNGCCAA; match class: 1 0.0429909572628257 1572 16 7 0.4375 0.0044529262086514
TF TF:M07044 Factor: HNF-1alpha; motif: NGNNAAWNATTAACNN 0.0429909572628257 1678 11 6 0.545454545454545 0.00357568533969011
TF TF:M07611_1 Factor: p54NRB; motif: GRNNNMGGATGRMNNCGGA; match class: 1 0.0429909572628257 1645 38 12 0.315789473684211 0.00729483282674772
TF TF:M11124 Factor: Olig3; motif: ANCAKMTGTT 0.0429909572628257 1103 20 7 0.35 0.00634632819582956
TF TF:M10002 Factor: NF-1C; motif: NYTGGCNNYNNGCCARN 0.0429909572628257 6760 71 41 0.577464788732394 0.00606508875739645
TF TF:M07338 Factor: Sox-10; motif: ACAAWGNN 0.0429909572628257 1695 7 5 0.714285714285714 0.00294985250737463
TF TF:M07354_1 Factor: Egr-1; motif: GCGGGGGCGG; match class: 1 0.0464502696539512 3561 126 40 0.317460317460317 0.011232799775344
TF TF:M04146_1 Factor: TFAP2A; motif: YGCCCNNRGGCN; match class: 1 0.0492557056831396 3800 116 39 0.336206896551724 0.0102631578947368
TF TF:M11728_1 Factor: NF-1C; motif: NTTGGCNNNNTGCCARN; match class: 1 0.0492557056831396 1129 16 6 0.375 0.00531443755535872
TF TF:M09640 Factor: NF-1C; motif: NYYTGGCWNNNNKCCMN 0.0492557056831396 2807 8 6 0.75 0.0021375133594585
TF TF:M03906 Factor: HIC2; motif: RTGCCCRNN 0.0492557056831396 3458 7 6 0.857142857142857 0.00173510699826489
TF TF:M11208_1 Factor: AP-4; motif: AWCAGCTGWT; match class: 1 0.0499010485213191 3864 28 14 0.5 0.0036231884057971
TF TF:M04146 Factor: TFAP2A; motif: YGCCCNNRGGCN 0.0499010485213191 6552 116 57 0.491379310344828 0.0086996336996337
TF TF:M04106_1 Factor: RUNX2; motif: NRACCGCAAACCGCAN; match class: 1 0.0499010485213191 8372 127 74 0.582677165354331 0.00883898709985667
TF TF:M01860 Factor: AP-4; motif: NCAGCTGYNGNCN 0.0499010485213191 5995 110 51 0.463636363636364 0.0085070892410342
TF TF:M08961 Factor: PPARGAMMA; motif: NWNTRGGTYANN 0.0499010485213191 5433 11 9 0.818181818181818 0.00165654334621756
TF TF:M01196 Factor: CTF1; motif: TGGCASCNNGCCAA 0.0499010485213191 4864 16 11 0.6875 0.00226151315789474
TF TF:M00034_1 Factor: p53; motif: GGACATGCCCGGGCATGTCY; match class: 1 0.0499010485213191 2646 6 5 0.833333333333333 0.0018896447467876
TF TF:M11729 Factor: NF-1C; motif: NTTGGCNNNNTGCCARN 0.0499010485213191 1738 16 7 0.4375 0.00402761795166858
TF TF:M10432_1 Factor: MAZ; motif: GGGMGGGGS; match class: 1 0.0499010485213191 4365 128 46 0.359375 0.0105383734249714
TF TF:M11097 Factor: Max; motif: MCATGTGNNNNMCATGTGN 0.0499010485213191 618 5 3 0.6 0.00485436893203883
TF TF:M12160_1 Factor: KLF15; motif: RCCMCRCCCMCN; match class: 1 0.0499010485213191 8031 129 73 0.565891472868217 0.00908977711368447
TF TF:M01837 Factor: FKLF; motif: BGGGNGGVMD 0.0499010485213191 5442 128 54 0.421875 0.00992282249173098
TF TF:M00915_1 Factor: AP-2; motif: SNNNCCNCAGGCN; match class: 1 0.0499010485213191 4551 126 47 0.373015873015873 0.010327400571303
TF TF:M00257 Factor: RREB-1; motif: CCCCAAACMMCCCC 0.0499010485213191 10516 129 89 0.689922480620155 0.00846329402814758
TF TF:M01651 Factor: P53; motif: RGRCATGYCYRGRCATGYYY 0.0499010485213191 2163 7 5 0.714285714285714 0.00231160425335183

pEpiTd15 transcription factor target enrichment of top program genes

TF enrichment of pEpiTd15
Possible regulators of activation of pEpiTd15 in epithelial cells. (Left) pearson correlation of TF expression and gene expression program activatity in Epithelial cells, (right) negative log p-value for enrichment of TF binding sites among top uniqueness weighted genes for the gene program. Blue bars indicate significant TFs meeting the following criteria: FDR<0.1, overlap > 2, Corr. R > 0, permutation based p-value < 0.05.