pEpiTd07 program activity among Epithelial cells

Activation of pEpiTd07
pEpiTd07 gene program activation in Epithelial cells. (Top) Activation of program across all epithelial cells. (Bottom) Activation in MMRd (left), MMRp (middle), and normal epithelial cells (right).

Epithelial cell compositional overview

Overview of epithelial cell composition
Overview of epithelial cell composition. A tSNE plot colored by: (left) MMR status of epithelial cells, (middle) specimen, (righ) epithelial cell sub-type.
Gene program activation of pEpiTd07 by different cell subtypes
pEpiTd07 gene program activation among cells of different subtypes (Epithelial cells).

pEpiTd07 top program genes

Top genes of pEpiTd07 program
Bar plots showing the top genes for each gene program, ranked by (left) unique weights, (right) raw weights.

pEpiTd07 gene-set enrichment of top program genes (using g-profiler)

GSEA wth gene profiler of pEpiTd07
pEpiTd07 gene-set enrichment summary using g-profiler webtool.
Source termID Name Padj Tsize Qsize Overlap Precision Recall
GO:BP GO:0006959 humoral immune response 1.7469407391308e-09 384 18 10 0.555555555555556 0.0260416666666667
GO:BP GO:0044278 cell wall disruption in other organism 5.75226878917038e-09 4 3 3 1 0.75
GO:BP GO:0019730 antimicrobial humoral response 5.1067504754438e-08 145 18 7 0.388888888888889 0.0482758620689655
GO:BP GO:0061844 antimicrobial humoral immune response mediated by antimicrobial peptide 9.1936865487869e-06 81 18 5 0.277777777777778 0.0617283950617284
GO:BP GO:0035821 modulation of process of other organism 7.38344688717799e-05 128 18 5 0.277777777777778 0.0390625
GO:BP GO:0002526 acute inflammatory response 0.000193768803011602 114 109 8 0.073394495412844 0.0701754385964912
GO:BP GO:0051707 response to other organism 0.000193768803011602 1592 18 10 0.555555555555556 0.00628140703517588
GO:BP GO:0043207 response to external biotic stimulus 0.000193768803011602 1594 18 10 0.555555555555556 0.00627352572145546
GO:BP GO:0006953 acute-phase response 0.000193768803011602 50 109 6 0.055045871559633 0.12
GO:BP GO:0009607 response to biotic stimulus 0.000209920602228183 1626 18 10 0.555555555555556 0.00615006150061501
GO:BP GO:0050830 defense response to Gram-positive bacterium 0.000679318182624963 101 18 4 0.222222222222222 0.0396039603960396
GO:BP GO:0002376 immune system process 0.00328643980275413 3278 19 12 0.631578947368421 0.0036607687614399
GO:BP GO:0006955 immune response 0.00328643980275413 2311 18 10 0.555555555555556 0.0043271311120727
GO:BP GO:0042742 defense response to bacterium 0.00328643980275413 349 18 5 0.277777777777778 0.0143266475644699
GO:BP GO:0044419 biological process involved in interspecies interaction between organisms 0.00328643980275413 2255 18 10 0.555555555555556 0.00443458980044346
GO:BP GO:0043434 response to peptide hormone 0.00328643980275413 462 3 3 1 0.00649350649350649
GO:BP GO:1901652 response to peptide 0.00469132625514894 551 3 3 1 0.00544464609800363
GO:BP GO:0019731 antibacterial humoral response 0.00475366337174542 62 18 3 0.166666666666667 0.0483870967741935
GO:BP GO:0010033 response to organic substance 0.00493829493141793 3412 22 13 0.590909090909091 0.00381008206330598
GO:BP GO:0009617 response to bacterium 0.00521030842241595 743 18 6 0.333333333333333 0.00807537012113055
GO:BP GO:0050829 defense response to Gram-negative bacterium 0.00521030842241595 88 84 5 0.0595238095238095 0.0568181818181818
GO:BP GO:0009605 response to external stimulus 0.00521030842241595 2944 19 11 0.578947368421053 0.00373641304347826
GO:BP GO:0050896 response to stimulus 0.0058937412211612 9407 82 60 0.731707317073171 0.00637822897842033
GO:BP GO:0098542 defense response to other organism 0.00842288124704723 1218 18 7 0.388888888888889 0.00574712643678161
GO:BP GO:0009725 response to hormone 0.0103685587657736 921 3 3 1 0.00325732899022801
GO:BP GO:0030449 regulation of complement activation 0.0104393723083538 110 17 3 0.176470588235294 0.0272727272727273
GO:BP GO:0008284 positive regulation of cell population proliferation 0.0116037907638868 982 3 3 1 0.00305498981670061
GO:BP GO:0006952 defense response 0.0122293662257876 1818 18 8 0.444444444444444 0.0044004400440044
GO:BP GO:0010243 response to organonitrogen compound 0.0145377137044276 1085 3 3 1 0.00276497695852535
GO:BP GO:0002251 organ or tissue specific immune response 0.0147989125289759 42 51 3 0.0588235294117647 0.0714285714285714
GO:BP GO:0043616 keratinocyte proliferation 0.0149444487680458 50 109 4 0.036697247706422 0.08
GO:BP GO:0002920 regulation of humoral immune response 0.0154491374197661 133 17 3 0.176470588235294 0.0225563909774436
GO:BP GO:0051094 positive regulation of developmental process 0.0154707160779863 1341 89 17 0.191011235955056 0.0126771066368382
GO:BP GO:1901698 response to nitrogen compound 0.0163768524375443 1172 3 3 1 0.00255972696245734
GO:BP GO:0045087 innate immune response 0.0171073890154191 998 18 6 0.333333333333333 0.00601202404809619
GO:BP GO:0006958 complement activation, classical pathway 0.0172024960583688 142 17 3 0.176470588235294 0.0211267605633803
GO:BP GO:0031099 regeneration 0.0182959599409618 206 81 6 0.0740740740740741 0.029126213592233
GO:BP GO:0002675 positive regulation of acute inflammatory response 0.0195709997626268 29 87 3 0.0344827586206897 0.103448275862069
GO:BP GO:0050793 regulation of developmental process 0.0204406777197172 2605 82 24 0.292682926829268 0.00921305182341651
GO:BP GO:0002455 humoral immune response mediated by circulating immunoglobulin 0.0205687268933328 156 17 3 0.176470588235294 0.0192307692307692
GO:BP GO:0033627 cell adhesion mediated by integrin 0.0210806185413514 73 36 3 0.0833333333333333 0.0410958904109589
GO:BP GO:0006950 response to stress 0.0210806185413514 4170 20 12 0.6 0.00287769784172662
GO:BP GO:0042127 regulation of cell population proliferation 0.0211218230733968 1774 5 4 0.8 0.00225479143179256
GO:BP GO:0050673 epithelial cell proliferation 0.0224768024282264 460 109 10 0.0917431192660551 0.0217391304347826
GO:BP GO:0045995 regulation of embryonic development 0.0231582984003292 63 44 3 0.0681818181818182 0.0476190476190476
GO:BP GO:1901700 response to oxygen-containing compound 0.0239477986952203 1739 122 24 0.19672131147541 0.0138010350776308
GO:BP GO:0006956 complement activation 0.0272100736183509 180 17 3 0.176470588235294 0.0166666666666667
GO:BP GO:0008283 cell population proliferation 0.0309513212019733 2054 5 4 0.8 0.00194741966893866
GO:BP GO:2000027 regulation of animal organ morphogenesis 0.0309513212019733 184 106 6 0.0566037735849057 0.0326086956521739
GO:BP GO:0009719 response to endogenous stimulus 0.0318855477591047 1683 3 3 1 0.0017825311942959
GO:BP GO:0051239 regulation of multicellular organismal process 0.0322289379645814 2861 88 26 0.295454545454545 0.00908773156239077
GO:BP GO:0051241 negative regulation of multicellular organismal process 0.0329071476500291 1160 57 11 0.192982456140351 0.00948275862068966
GO:BP GO:0006954 inflammatory response 0.0351049960711832 794 109 13 0.119266055045872 0.0163727959697733
GO:BP GO:0060429 epithelium development 0.0383797257555415 1325 111 18 0.162162162162162 0.0135849056603774
GO:BP GO:0046849 bone remodeling 0.0383797257555415 93 89 4 0.0449438202247191 0.043010752688172
GO:BP GO:0019724 B cell mediated immunity 0.0383797257555415 226 17 3 0.176470588235294 0.0132743362831858
GO:BP GO:0016064 immunoglobulin mediated immune response 0.0383797257555415 223 17 3 0.176470588235294 0.0134529147982063
GO:BP GO:0045597 positive regulation of cell differentiation 0.0393445321182609 879 89 12 0.134831460674157 0.0136518771331058
GO:BP GO:0042221 response to chemical 0.0393445321182609 4781 22 13 0.590909090909091 0.0027190964233424
GO:BP GO:0032501 multicellular organismal process 0.043537307263252 7946 90 54 0.6 0.00679587213692424
GO:BP GO:0046329 negative regulation of JNK cascade 0.0444427752751463 39 106 3 0.0283018867924528 0.0769230769230769
GO:BP GO:1901617 organic hydroxy compound biosynthetic process 0.0454660282886512 254 121 7 0.0578512396694215 0.0275590551181102
GO:BP GO:0010837 regulation of keratinocyte proliferation 0.046221982595725 39 109 3 0.0275229357798165 0.0769230769230769
GO:BP GO:0015718 monocarboxylic acid transport 0.046221982595725 189 82 5 0.0609756097560976 0.0264550264550265
GO:BP GO:0015849 organic acid transport 0.0482231101761569 331 122 8 0.0655737704918033 0.0241691842900302
GO:BP GO:0002673 regulation of acute inflammatory response 0.0482231101761569 50 87 3 0.0344827586206897 0.06
GO:BP GO:0046942 carboxylic acid transport 0.0487785466817079 294 82 6 0.0731707317073171 0.0204081632653061
GO:BP GO:0045616 regulation of keratinocyte differentiation 0.0487785466817079 43 109 3 0.0275229357798165 0.0697674418604651
GO:BP GO:0030155 regulation of cell adhesion 0.0487785466817079 748 97 11 0.11340206185567 0.0147058823529412
GO:BP GO:0001738 morphogenesis of a polarized epithelium 0.0487785466817079 152 106 5 0.0471698113207547 0.0328947368421053
GO:BP GO:0007399 nervous system development 0.0493229359327963 2449 114 27 0.236842105263158 0.0110249081257656
GO:CC GO:0005615 extracellular space 9.27055814531836e-06 3594 19 15 0.789473684210526 0.00417362270450751
GO:CC GO:1903561 extracellular vesicle 3.3260187517566e-05 2261 14 10 0.714285714285714 0.00442282176028306
GO:CC GO:0070062 extracellular exosome 3.3260187517566e-05 2176 14 10 0.714285714285714 0.00459558823529412
GO:CC GO:0043230 extracellular organelle 3.3260187517566e-05 2263 14 10 0.714285714285714 0.00441891294741494
GO:CC GO:0005576 extracellular region 5.19230830831883e-05 4567 21 16 0.761904761904762 0.00350339391285308
GO:CC GO:0031982 vesicle 0.00126203237829676 4055 15 11 0.733333333333333 0.00271270036991369
GO:CC GO:0031225 anchored component of membrane 0.0123556807986152 174 97 6 0.0618556701030928 0.0344827586206897
GO:CC GO:0031012 extracellular matrix 0.0431962025316458 562 89 9 0.101123595505618 0.0160142348754448
GO:CC GO:0030312 external encapsulating structure 0.0431962025316458 563 89 9 0.101123595505618 0.0159857904085258
GO:MF GO:0070492 oligosaccharide binding 1.90186681258493e-07 15 3 3 1 0.2
GO:MF GO:0042834 peptidoglycan binding 1.90186681258493e-07 18 3 3 1 0.166666666666667
GO:MF GO:0005539 glycosaminoglycan binding 0.000344734491634493 238 3 3 1 0.0126050420168067
GO:MF GO:0030246 carbohydrate binding 0.000399534787189738 275 3 3 1 0.0109090909090909
GO:MF GO:0017147 Wnt-protein binding 0.0229253505115581 30 89 3 0.0337078651685393 0.1
GO:MF GO:0046625 sphingolipid binding 0.0229253505115581 27 101 3 0.0297029702970297 0.111111111111111
GO:MF GO:0060089 molecular transducer activity 0.0229253505115581 1458 3 3 1 0.00205761316872428
GO:MF GO:0038023 signaling receptor activity 0.0229253505115581 1458 3 3 1 0.00205761316872428
GO:MF GO:0097367 carbohydrate derivative binding 0.0374315957274293 2285 8 5 0.625 0.00218818380743982
REAC REAC:R-HSA-6803157 Antimicrobial peptides 0.00277602751670245 80 18 4 0.222222222222222 0.05
REAC REAC:R-HSA-168249 Innate Immune System 0.0421064354165665 1073 20 8 0.4 0.00745573159366263
REAC REAC:R-HSA-977606 Regulation of Complement cascade 0.0421064354165665 101 17 3 0.176470588235294 0.0297029702970297
REAC REAC:R-HSA-166658 Complement cascade 0.04439069115286 111 17 3 0.176470588235294 0.027027027027027

pEpiTd07 transcription factor target enrichment of top program genes

TF enrichment of pEpiTd07
Possible regulators of activation of pEpiTd07 in epithelial cells. (Left) pearson correlation of TF expression and gene expression program activatity in Epithelial cells, (right) negative log p-value for enrichment of TF binding sites among top uniqueness weighted genes for the gene program. Blue bars indicate significant TFs meeting the following criteria: FDR<0.1, overlap > 2, Corr. R > 0, permutation based p-value < 0.05.