pEpiTp24 program activity among Epithelial cells

Activation of pEpiTp24
pEpiTp24 gene program activation in Epithelial cells. (Top) Activation of program across all epithelial cells. (Bottom) Activation in MMRd (left), MMRp (middle), and normal epithelial cells (right).

Epithelial cell compositional overview

Overview of epithelial cell composition
Overview of epithelial cell composition. A tSNE plot colored by: (left) MMR status of epithelial cells, (middle) specimen, (righ) epithelial cell sub-type.
Gene program activation of pEpiTp24 by different cell subtypes
pEpiTp24 gene program activation among cells of different subtypes (Epithelial cells).

pEpiTp24 top program genes

Top genes of pEpiTp24 program
Bar plots showing the top genes for each gene program, ranked by (left) unique weights, (right) raw weights.

pEpiTp24 gene-set enrichment of top program genes (using g-profiler)

GSEA wth gene profiler of pEpiTp24
pEpiTp24 gene-set enrichment summary using g-profiler webtool.
Source termID Name Padj Tsize Qsize Overlap Precision Recall
GO:BP GO:0001501 skeletal system development 0.0104796293778256 504 135 15 0.111111111111111 0.0297619047619048
GO:BP GO:0035265 organ growth 0.0104796293778256 182 131 9 0.0687022900763359 0.0494505494505494
GO:BP GO:0043009 chordate embryonic development 0.0433135957824131 616 59 9 0.152542372881356 0.0146103896103896
GO:BP GO:0090596 sensory organ morphogenesis 0.0433135957824131 262 126 9 0.0714285714285714 0.0343511450381679
GO:BP GO:0060419 heart growth 0.0433135957824131 111 125 6 0.048 0.0540540540540541
GO:BP GO:0048592 eye morphogenesis 0.0433135957824131 155 27 4 0.148148148148148 0.0258064516129032
GO:BP GO:0021510 spinal cord development 0.0433135957824131 104 48 4 0.0833333333333333 0.0384615384615385
GO:BP GO:0021915 neural tube development 0.0433135957824131 158 59 5 0.0847457627118644 0.0316455696202532
GO:BP GO:0021953 central nervous system neuron differentiation 0.0433135957824131 188 27 4 0.148148148148148 0.0212765957446809
GO:BP GO:0045324 late endosome to vacuole transport 0.0433135957824131 24 83 3 0.036144578313253 0.125
GO:BP GO:0009792 embryo development ending in birth or egg hatching 0.0451835796582736 635 59 9 0.152542372881356 0.0141732283464567
GO:BP GO:0048736 appendage development 0.0474522038364121 176 32 4 0.125 0.0227272727272727
GO:BP GO:0060173 limb development 0.0474522038364121 176 32 4 0.125 0.0227272727272727
GO:BP GO:0003002 regionalization 0.0474522038364121 338 48 6 0.125 0.0177514792899408
GO:CC GO:0043230 extracellular organelle 0.000807417949522316 2263 27 13 0.481481481481481 0.00574458683163942
GO:CC GO:1903561 extracellular vesicle 0.000807417949522316 2261 27 13 0.481481481481481 0.00574966828836798
GO:CC GO:0070062 extracellular exosome 0.0014695168473169 2176 26 12 0.461538461538462 0.00551470588235294
GO:CC GO:0031982 vesicle 0.00222939909623676 4055 27 16 0.592592592592593 0.00394574599260173
GO:CC GO:0005737 cytoplasm 0.00260124934418395 11911 138 109 0.789855072463768 0.0091512047687012
GO:CC GO:0043227 membrane-bounded organelle 0.00461390320908676 12858 138 114 0.826086956521739 0.00886607559496034
GO:CC GO:0012505 endomembrane system 0.0049000417274051 4640 138 54 0.391304347826087 0.0116379310344828
GO:CC GO:0043229 intracellular organelle 0.0049000417274051 12491 132 107 0.810606060606061 0.00856616764070131
GO:CC GO:0043226 organelle 0.00654272056759548 14008 138 120 0.869565217391304 0.0085665334094803
GO:CC GO:0031984 organelle subcompartment 0.0135606749264575 1773 107 22 0.205607476635514 0.0124083474337281
GO:CC GO:0043231 intracellular membrane-bounded organelle 0.0140157819194478 11296 131 97 0.740458015267176 0.0085871104815864
GO:CC GO:0031300 intrinsic component of organelle membrane 0.0195359717048756 413 107 9 0.0841121495327103 0.0217917675544794
GO:CC GO:0098827 endoplasmic reticulum subcompartment 0.0218442035372306 1122 100 15 0.15 0.0133689839572193
GO:CC GO:0010008 endosome membrane 0.0218442035372306 505 131 11 0.083969465648855 0.0217821782178218
GO:CC GO:0005789 endoplasmic reticulum membrane 0.0218442035372306 1117 100 15 0.15 0.0134288272157565
GO:CC GO:0005768 endosome 0.0221596425623875 987 138 17 0.123188405797101 0.0172239108409321
GO:CC GO:0042175 nuclear outer membrane-endoplasmic reticulum membrane network 0.0224646208016402 1139 100 15 0.15 0.0131694468832309
GO:CC GO:0098563 intrinsic component of synaptic vesicle membrane 0.0252418937828555 47 84 3 0.0357142857142857 0.0638297872340425
GO:CC GO:0005622 intracellular anatomical structure 0.0252418937828555 14790 132 117 0.886363636363636 0.00791075050709939
GO:CC GO:0099501 exocytic vesicle membrane 0.0264631746412045 111 84 4 0.0476190476190476 0.036036036036036
GO:CC GO:0005615 extracellular space 0.0264631746412045 3594 26 12 0.461538461538462 0.00333889816360601
GO:CC GO:0030672 synaptic vesicle membrane 0.0264631746412045 111 84 4 0.0476190476190476 0.036036036036036
GO:CC GO:0030658 transport vesicle membrane 0.0371589635534487 209 84 5 0.0595238095238095 0.0239234449760766
GO:CC GO:0005829 cytosol 0.0371589635534487 5303 113 46 0.407079646017699 0.0086743352819159
GO:CC GO:0097708 intracellular vesicle 0.0371589635534487 2428 138 30 0.217391304347826 0.0123558484349259
GO:CC GO:0005783 endoplasmic reticulum 0.0415521931847192 1982 100 20 0.2 0.0100908173562059
GO:CC GO:0005770 late endosome 0.0415521931847192 275 131 7 0.0534351145038168 0.0254545454545455
GO:CC GO:0031902 late endosome membrane 0.0415521931847192 140 131 5 0.0381679389312977 0.0357142857142857
TF TF:M03878_1 Factor: MIBP1; motif: WNWCCCCAGCTR; match class: 1 0.00139700177040795 286 5 4 0.8 0.013986013986014
TF TF:M10529 Factor: Sp1; motif: RGGGMGGRGSNGGGG 0.00149445084733159 6891 50 35 0.7 0.00507908866637643
TF TF:M08487 Factor: GCMa:Erg; motif: ATGCGGGCGGAARKG 0.00420654387667176 13942 54 52 0.962962962962963 0.00372973748386171
TF TF:M07277 Factor: BTEB2; motif: RGGGNGKGGN 0.0277577209276982 8492 137 82 0.598540145985402 0.00965614696184644
TF TF:M00144_1 Factor: Pax-5; motif: RRMSWGANWYCTNRAGCGKRACSRYNSM; match class: 1 0.0277577209276982 7128 83 49 0.590361445783133 0.00687429854096521
TF TF:M09973_1 Factor: CPBP; motif: GNNRGGGHGGGGNNGGGRN; match class: 1 0.0277577209276982 6607 57 34 0.596491228070175 0.00514605721204783
TF TF:M09969 Factor: EKLF; motif: NGGGYGKGGCNNGG 0.0277577209276982 4589 58 28 0.482758620689655 0.00610154717803443
TF TF:M07226 Factor: SP1; motif: NCCCCKCCCCC 0.0277577209276982 8292 137 81 0.591240875912409 0.0097684515195369
TF TF:M09972 Factor: BTEB2; motif: WGGGTGKGGCNGGN 0.0277577209276982 9217 49 37 0.755102040816326 0.00401432136269936
TF TF:M08487_1 Factor: GCMa:Erg; motif: ATGCGGGCGGAARKG; match class: 1 0.0277577209276982 8790 50 37 0.74 0.00420932878270762
TF TF:M07617 Factor: Sp4; motif: SCCCCKCCCCCSN 0.0277577209276982 3595 136 44 0.323529411764706 0.0122392211404729
TF TF:M10096_1 Factor: TCF-4; motif: NCCTTTGATSTNN; match class: 1 0.0277577209276982 69 51 4 0.0784313725490196 0.0579710144927536
TF TF:M00333_1 Factor: ZF5; motif: NRNGNGCGCGCWN; match class: 1 0.0277577209276982 12401 42 38 0.904761904761905 0.00306426901056366
TF TF:M09973 Factor: CPBP; motif: GNNRGGGHGGGGNNGGGRN 0.0300213857034929 10853 52 42 0.807692307692308 0.00386989772413158
TF TF:M03847 Factor: Sox-15; motif: ACAAWGGG 0.0300213857034929 1583 54 14 0.259259259259259 0.00884396715097915
TF TF:M10072 Factor: sp4; motif: NNGNARGRGGCGGRGCNNRR 0.0300213857034929 10494 137 95 0.693430656934307 0.00905279207166
TF TF:M03920 Factor: SP1; motif: RCCMCRCCCMC 0.0300213857034929 7283 58 36 0.620689655172414 0.00494301798709323
TF TF:M09834_1 Factor: ZNF148; motif: NNNNNNCCNNCCCCTCCCCCACCCN; match class: 1 0.0300213857034929 2869 57 20 0.350877192982456 0.0069710700592541
TF TF:M00932_1 Factor: Sp1; motif: NNGGGGCGGGGNN; match class: 1 0.0300213857034929 6047 136 63 0.463235294117647 0.0104183892839425
TF TF:M07277_1 Factor: BTEB2; motif: RGGGNGKGGN; match class: 1 0.0300213857034929 4149 132 47 0.356060606060606 0.0113280308508074
TF TF:M10112_1 Factor: Miz-1; motif: NNRGGWGGGGGAGGGGMRR; match class: 1 0.0317789422119948 4075 52 23 0.442307692307692 0.0056441717791411
TF TF:M10432_1 Factor: MAZ; motif: GGGMGGGGS; match class: 1 0.0317789422119948 4365 55 25 0.454545454545455 0.00572737686139748
TF TF:M01303 Factor: SP1; motif: GGGGYGGGGNS 0.0330207076580926 7898 55 36 0.654545454545455 0.00455811597872879
TF TF:M01175 Factor: CKROX; motif: SCCCTCCCC 0.0330207076580926 7292 132 70 0.53030303030303 0.00959956116291827
TF TF:M07409 Factor: BTEB2; motif: GCCCCRCCCH 0.033071418866679 5198 132 54 0.409090909090909 0.0103886110042324
TF TF:M09970 Factor: KLF3; motif: NNNNNNGGGCGGGGCNNGN 0.033071418866679 7728 55 35 0.636363636363636 0.00452898550724638
TF TF:M10144 Factor: ZNF341; motif: GRGGRRNRGNRGNRGGAASAGC 0.033071418866679 4766 18 12 0.666666666666667 0.00251783466219052
TF TF:M07397_1 Factor: ZBP89; motif: CCCCKCCCCCNN; match class: 1 0.033071418866679 2951 54 19 0.351851851851852 0.00643849542527957
TF TF:M00083 Factor: MZF-1; motif: NGNGGGGA 0.033071418866679 2867 8 6 0.75 0.00209277990931287
TF TF:M11482 Factor: AP-2gamma; motif: NSCCYNNRGSN 0.033071418866679 6696 53 31 0.584905660377358 0.00462962962962963
TF TF:M00931 Factor: Sp1; motif: GGGGCGGGGC 0.033071418866679 10281 55 42 0.763636363636364 0.00408520571928801
TF TF:M09918 Factor: Pet-1; motif: GCNGGAAGYG 0.033071418866679 13610 138 114 0.826086956521739 0.00837619397501837
TF TF:M09967 Factor: AP-2rep; motif: NGGGGCGGGGC 0.033071418866679 4023 132 45 0.340909090909091 0.0111856823266219
TF TF:M04109 Factor: RUNX3; motif: NRACCGCAAACCGCAN 0.033071418866679 9770 44 34 0.772727272727273 0.00348004094165814
TF TF:M00008_1 Factor: Sp1; motif: GGGGCGGGGT; match class: 1 0.033071418866679 4937 132 52 0.393939393939394 0.0105327121733846
TF TF:M07615_1 Factor: Sp3; motif: GGGGCGGGGSNN; match class: 1 0.033071418866679 2885 136 36 0.264705882352941 0.0124783362218371
TF TF:M10529_1 Factor: Sp1; motif: RGGGMGGRGSNGGGG; match class: 1 0.033071418866679 2816 48 17 0.354166666666667 0.00603693181818182
TF TF:M03807 Factor: SP2; motif: GNNGGGGGCGGGGSN 0.033071418866679 8415 113 68 0.601769911504425 0.00808080808080808
TF TF:M00933 Factor: Sp1; motif: CCCCGCCCCN 0.033071418866679 9715 49 37 0.755102040816326 0.00380854348944931
TF TF:M00933_1 Factor: Sp1; motif: CCCCGCCCCN; match class: 1 0.033071418866679 5166 132 54 0.409090909090909 0.0104529616724739
TF TF:M00932 Factor: Sp1; motif: NNGGGGCGGGGNN 0.033071418866679 10419 138 94 0.681159420289855 0.00902197907668682
TF TF:M06948 Factor: Sp2; motif: TGGGCGCGCCCA 0.033071418866679 8864 111 69 0.621621621621622 0.00778429602888087
TF TF:M06948_1 Factor: Sp2; motif: TGGGCGCGCCCA; match class: 1 0.033071418866679 6205 87 44 0.505747126436782 0.00709105560032232
TF TF:M01073 Factor: HIC1; motif: NNNGGKTGCCCSNNNNNN 0.0333705163419218 4088 54 23 0.425925925925926 0.00562622309197652
TF TF:M04076 Factor: POU3F1; motif: WTATGCWAATNN 0.0344021107454763 610 54 8 0.148148148148148 0.0131147540983607
TF TF:M00978_1 Factor: LEF-1,; motif: MCTTTGWTSNY; match class: 1 0.0347416692938701 118 51 4 0.0784313725490196 0.0338983050847458
TF TF:M01705_1 Factor: TCF-4; motif: NCTTTGATS; match class: 1 0.0347803970742102 120 51 4 0.0784313725490196 0.0333333333333333
TF TF:M08205 Factor: E2F-1:Elk-1; motif: SGCGCSNNAMCGGAAGT 0.0347803970742102 14429 55 51 0.927272727272727 0.00353454847875806
TF TF:M01308_1 Factor: Sox-4; motif: AACAAWGR; match class: 1 0.0347803970742102 107 23 3 0.130434782608696 0.0280373831775701
TF TF:M04953_1 Factor: Sp1; motif: GGNDGGRGGCGGGG; match class: 1 0.0362292992004485 4065 55 23 0.418181818181818 0.00565805658056581
TF TF:M07039 Factor: ETF; motif: CCCCGCCCCYN 0.0362292992004485 16384 44 44 1 0.002685546875
TF TF:M00806_1 Factor: NF-1; motif: NTGGNNNNNNGCCAANN; match class: 1 0.0362292992004485 562 46 7 0.152173913043478 0.0124555160142349
TF TF:M07208 Factor: EGR1; motif: NCNCCGCCCCCGCN 0.0362292992004485 6183 55 30 0.545454545454545 0.00485201358563804
TF TF:M04083 Factor: POU3F4; motif: TATGCWAAT 0.0362292992004485 473 54 7 0.12962962962963 0.0147991543340381
TF TF:M07602_1 Factor: CP2; motif: NNNNCCAGNCNN; match class: 1 0.0370176299303777 4627 38 19 0.5 0.00410633239680138
TF TF:M09837_1 Factor: ZNF449; motif: NNNAGCCCARCC; match class: 1 0.0370176299303777 764 110 13 0.118181818181818 0.0170157068062827
TF TF:M00196 Factor: Sp1; motif: NGGGGGCGGGGYN 0.0370176299303777 10233 42 33 0.785714285714286 0.00322486074464966
TF TF:M07289 Factor: GKLF; motif: NNNRGGNGNGGSN 0.0370176299303777 14646 50 47 0.94 0.00320906732213574
TF TF:M08884 Factor: GCM; motif: NNNNATGMGGGN 0.0370176299303777 1185 111 17 0.153153153153153 0.0143459915611814
TF TF:M07615 Factor: Sp3; motif: GGGGCGGGGSNN 0.0375863347413219 6807 137 67 0.489051094890511 0.00984280887321875
TF TF:M11882_1 Factor: pax-6; motif: NYACGCNYSANYGMNCN; match class: 1 0.0375863347413219 11423 87 66 0.758620689655172 0.00577781668563425
TF TF:M06293 Factor: ZNF742; motif: NGGGACGAGGSC 0.0375863347413219 234 79 6 0.0759493670886076 0.0256410256410256
TF TF:M11603 Factor: TCF-1; motif: ACATCGRGRCGCTGW 0.0381435423362798 16361 42 42 1 0.00256708025181835
TF TF:M11603_1 Factor: TCF-1; motif: ACATCGRGRCGCTGW; match class: 1 0.0381435423362798 13184 42 38 0.904761904761905 0.00288228155339806
TF TF:M03824 Factor: GCMb; motif: RCCCKCAT 0.0381435423362798 1550 111 20 0.18018018018018 0.0129032258064516
TF TF:M09723_1 Factor: BTEB1; motif: GGGGGCGGGGCNGSGGGNGS; match class: 1 0.0381435423362798 6113 49 27 0.551020408163265 0.00441681662031736
TF TF:M07297_1 Factor: MAZ; motif: CCCTCCCYCYN; match class: 1 0.0381435423362798 685 11 4 0.363636363636364 0.00583941605839416
TF TF:M09862 Factor: ZNF614; motif: NCYCWGCCYYNNN 0.0383003548811673 9361 137 85 0.62043795620438 0.00908022647153082
TF TF:M04932 Factor: Sp2; motif: AAGGGGCGG 0.0383003548811673 2593 138 33 0.239130434782609 0.0127265715387582
TF TF:M10435 Factor: Sp2; motif: GGGGCGGGG 0.0415423988391223 7696 137 73 0.532846715328467 0.00948544698544699
TF TF:M07063 Factor: Sp1; motif: GGGGCGGGGC 0.0415423988391223 7696 137 73 0.532846715328467 0.00948544698544699
TF TF:M00425_1 Factor: E2F; motif: TTTCGCGC; match class: 1 0.0415423988391223 5355 42 22 0.523809523809524 0.00410830999066293
TF TF:M12173_1 Factor: GKLF; motif: NNCCMCRCCCN; match class: 1 0.0415423988391223 4965 59 27 0.457627118644068 0.0054380664652568
TF TF:M09918_1 Factor: Pet-1; motif: GCNGGAAGYG; match class: 1 0.0415423988391223 7159 56 33 0.589285714285714 0.00460958234390278
TF TF:M04934_1 Factor: TR4; motif: ACCCCGS; match class: 1 0.0415423988391223 15478 42 41 0.976190476190476 0.00264892104923117
TF TF:M10067 Factor: Sox-2; motif: NNCCTTTGTNYYN 0.0415571262498128 6131 59 31 0.525423728813559 0.00505627140760072
TF TF:M09723 Factor: BTEB1; motif: GGGGGCGGGGCNGSGGGNGS 0.0416166631639811 10156 49 37 0.755102040816326 0.00364316660102403
TF TF:M09819_1 Factor: SOX10; motif: NNAACAAAGNN; match class: 1 0.0416591875245579 1114 133 18 0.135338345864662 0.0161579892280072
TF TF:M09970_1 Factor: KLF3; motif: NNNNNNGGGCGGGGCNNGN; match class: 1 0.0420631508675434 3783 57 22 0.385964912280702 0.00581549035157283
TF TF:M10071_1 Factor: Sp1; motif: NGGGGGCGGGGCCNGGGGGGGG; match class: 1 0.0430262673346464 4623 57 25 0.43859649122807 0.00540774388924941
TF TF:M01175_1 Factor: CKROX; motif: SCCCTCCCC; match class: 1 0.0439964217536599 2679 136 33 0.242647058823529 0.0123180291153415
TF TF:M09969_1 Factor: EKLF; motif: NGGGYGKGGCNNGG; match class: 1 0.0455993530442261 1489 119 20 0.168067226890756 0.0134318334452653
TF TF:M07339 Factor: Staf; motif: AMNTCCCAGAATGCCT 0.0473327395274283 1178 108 16 0.148148148148148 0.0135823429541596
TF TF:M03922 Factor: SP4; motif: NWRGCCACGCCCMCTYN 0.0473327395274283 6046 58 30 0.517241379310345 0.00496195831955012
TF TF:M12227 Factor: ZIC4; motif: NNCCNCCCRYNGYGN 0.0473327395274283 11063 42 34 0.80952380952381 0.00307330742113351
TF TF:M09835_1 Factor: ZNF189; motif: RSNNGGAACAGMR; match class: 1 0.0473327395274283 358 10 3 0.3 0.00837988826815642
TF TF:M11639_1 Factor: Sox-4; motif: NAACAAAGRN; match class: 1 0.0473327395274283 347 23 4 0.173913043478261 0.0115273775216138
TF TF:M00139 Factor: AhR; motif: CCYCNRRSTNGCGTGASA 0.0473327395274283 3728 55 21 0.381818181818182 0.00563304721030043
TF TF:M09734_1 Factor: ZNF692; motif: SYNGGSCCCASCCNC; match class: 1 0.0473327395274283 6255 54 29 0.537037037037037 0.00463629096722622
TF TF:M00695_1 Factor: ETF; motif: GVGGMGG; match class: 1 0.0473327395274283 7032 49 29 0.591836734693878 0.00412400455062571
TF TF:M11601 Factor: TCF-1; motif: ACATCGRGRCGCTGW 0.0473327395274283 14823 42 40 0.952380952380952 0.00269850907373676
TF TF:M00342 Factor: Oct-1; motif: TATGCAAATN 0.0473327395274283 2966 54 18 0.333333333333333 0.00606877950101146
TF TF:M04826 Factor: p300; motif: ACNTCCG 0.048073944603758 17806 115 113 0.982608695652174 0.00634617544647872

pEpiTp24 transcription factor target enrichment of top program genes

TF enrichment of pEpiTp24
Possible regulators of activation of pEpiTp24 in epithelial cells. (Left) pearson correlation of TF expression and gene expression program activatity in Epithelial cells, (right) negative log p-value for enrichment of TF binding sites among top uniqueness weighted genes for the gene program. Blue bars indicate significant TFs meeting the following criteria: FDR<0.1, overlap > 2, Corr. R > 0, permutation based p-value < 0.05.