pTNI14 program activity among T/NK/ILC cells

Activation of pTNI14
pTNI14 gene program activation in T/NK/ILC cells.
Gene program activation of pTNI14 by different cell subtypes
pTNI14 gene program activation among cells of different subtypes (T/NK/ILC cells).

pTNI14 top program genes

Top genes of pTNI14 program
Bar plots showing the top genes for each gene program, ranked by (left) unique weights, (right) raw weights.

pTNI14 gene-set enrichment of top program genes (using g-profiler)

GSEA wth gene profiler of pTNI14
pTNI14 gene-set enrichment summary using g-profiler webtool.
Source termID Name Padj Tsize Qsize Overlap Precision Recall
GO:BP GO:0002727 regulation of natural killer cell cytokine production 2.41478535252382e-05 9 13 3 0.230769230769231 0.333333333333333
GO:BP GO:0002370 natural killer cell cytokine production 2.41478535252382e-05 9 13 3 0.230769230769231 0.333333333333333
GO:BP GO:0002715 regulation of natural killer cell mediated immunity 0.000104025673754932 45 20 4 0.2 0.0888888888888889
GO:BP GO:0002228 natural killer cell mediated immunity 0.000349541778234843 65 20 4 0.2 0.0615384615384615
GO:BP GO:0002440 production of molecular mediator of immune response 0.00048332789818523 189 19 5 0.263157894736842 0.0264550264550265
GO:BP GO:0035589 G protein-coupled purinergic nucleotide receptor signaling pathway 0.000638844033616346 15 31 3 0.0967741935483871 0.2
GO:BP GO:0035590 purinergic nucleotide receptor signaling pathway 0.000638844033616346 34 50 4 0.08 0.117647058823529
GO:BP GO:0002717 positive regulation of natural killer cell mediated immunity 0.00114400277062893 30 20 3 0.15 0.1
GO:BP GO:0032814 regulation of natural killer cell activation 0.00163353829319831 35 20 3 0.15 0.0857142857142857
GO:BP GO:0042269 regulation of natural killer cell mediated cytotoxicity 0.00198702598975774 41 20 3 0.15 0.0731707317073171
GO:BP GO:0002700 regulation of production of molecular mediator of immune response 0.00214898258589442 148 19 4 0.210526315789474 0.027027027027027
GO:BP GO:0045088 regulation of innate immune response 0.00233712636258481 309 20 5 0.25 0.0161812297734628
GO:BP GO:0002706 regulation of lymphocyte mediated immunity 0.00252744915813892 151 20 4 0.2 0.0264900662251656
GO:BP GO:0014074 response to purine-containing compound 0.00296751110314654 152 70 6 0.0857142857142857 0.0394736842105263
GO:BP GO:0002718 regulation of cytokine production involved in immune response 0.00296751110314654 85 13 3 0.230769230769231 0.0352941176470588
GO:BP GO:0071318 cellular response to ATP 0.00296751110314654 18 61 3 0.0491803278688525 0.166666666666667
GO:BP GO:0002367 cytokine production involved in immune response 0.00354288990392554 94 13 3 0.230769230769231 0.0319148936170213
GO:BP GO:0042267 natural killer cell mediated cytotoxicity 0.00378883474591207 62 20 3 0.15 0.0483870967741935
GO:BP GO:0002831 regulation of response to biotic stimulus 0.00531361679397263 415 20 5 0.25 0.0120481927710843
GO:BP GO:0002703 regulation of leukocyte mediated immunity 0.00531361679397263 205 20 4 0.2 0.0195121951219512
GO:BP GO:0001910 regulation of leukocyte mediated cytotoxicity 0.00531361679397263 74 20 3 0.15 0.0405405405405405
GO:BP GO:0002699 positive regulation of immune effector process 0.00574104962295087 215 20 4 0.2 0.0186046511627907
GO:BP GO:0030101 natural killer cell activation 0.0068925439991099 84 20 3 0.15 0.0357142857142857
GO:BP GO:0002697 regulation of immune effector process 0.00759898068732017 461 20 5 0.25 0.0108459869848156
GO:BP GO:0002702 positive regulation of production of molecular mediator of immune response 0.00870925397726988 100 19 3 0.157894736842105 0.03
GO:BP GO:0031341 regulation of cell killing 0.00870925397726988 93 20 3 0.15 0.032258064516129
GO:BP GO:0050777 negative regulation of immune response 0.00977491336992732 157 13 3 0.230769230769231 0.0191082802547771
GO:BP GO:0032649 regulation of interferon-gamma production 0.0100669774884877 110 19 3 0.157894736842105 0.0272727272727273
GO:BP GO:0046683 response to organophosphorus 0.0100669774884877 137 70 5 0.0714285714285714 0.0364963503649635
GO:BP GO:0002708 positive regulation of lymphocyte mediated immunity 0.0100669774884877 104 20 3 0.15 0.0288461538461538
GO:BP GO:0033198 response to ATP 0.0100726309069489 34 61 3 0.0491803278688525 0.0882352941176471
GO:BP GO:0001909 leukocyte mediated cytotoxicity 0.0103369770068311 107 20 3 0.15 0.0280373831775701
GO:BP GO:0032609 interferon-gamma production 0.0104110502267168 115 19 3 0.157894736842105 0.0260869565217391
GO:BP GO:0002376 immune system process 0.0104110502267168 3278 20 11 0.55 0.00335570469798658
GO:BP GO:0002683 negative regulation of immune system process 0.0107256764295076 420 25 5 0.2 0.0119047619047619
GO:BP GO:0002705 positive regulation of leukocyte mediated immunity 0.0122312149248396 122 20 3 0.15 0.0245901639344262
GO:BP GO:0050896 response to stimulus 0.0142690900723851 9407 61 45 0.737704918032787 0.00478367173381524
GO:BP GO:0002449 lymphocyte mediated immunity 0.0183441437713155 354 20 4 0.2 0.0112994350282486
GO:BP GO:0051251 positive regulation of lymphocyte activation 0.0195026500603568 365 20 4 0.2 0.010958904109589
GO:BP GO:0051241 negative regulation of multicellular organismal process 0.0195026500603568 1160 55 11 0.2 0.00948275862068966
GO:BP GO:0051093 negative regulation of developmental process 0.0195026500603568 977 14 5 0.357142857142857 0.00511770726714432
GO:BP GO:0007169 transmembrane receptor protein tyrosine kinase signaling pathway 0.0196873499759299 760 5 3 0.6 0.00394736842105263
GO:BP GO:0048585 negative regulation of response to stimulus 0.0199857467932955 1733 13 6 0.461538461538462 0.00346220427005193
GO:BP GO:0007167 enzyme linked receptor protein signaling pathway 0.0211749023079752 1103 8 4 0.5 0.00362647325475975
GO:BP GO:0031347 regulation of defense response 0.0213144433965926 704 20 5 0.25 0.00710227272727273
GO:BP GO:0007166 cell surface receptor signaling pathway 0.0213144433965926 3210 33 14 0.424242424242424 0.0043613707165109
GO:BP GO:0001906 cell killing 0.0217938823292896 175 20 3 0.15 0.0171428571428571
GO:BP GO:0002696 positive regulation of leukocyte activation 0.0230134177738179 412 20 4 0.2 0.00970873786407767
GO:BP GO:0002682 regulation of immune system process 0.0236677204917672 1617 25 8 0.32 0.00494743351886209
GO:BP GO:0001819 positive regulation of cytokine production 0.0236677204917672 452 19 4 0.210526315789474 0.00884955752212389
GO:BP GO:0050867 positive regulation of cell activation 0.0236677204917672 424 20 4 0.2 0.00943396226415094
GO:BP GO:0001817 regulation of cytokine production 0.0244636210706913 806 19 5 0.263157894736842 0.00620347394540943
GO:BP GO:0007204 positive regulation of cytosolic calcium ion concentration 0.025293744745553 325 67 6 0.0895522388059701 0.0184615384615385
GO:BP GO:0046649 lymphocyte activation 0.025293744745553 771 20 5 0.25 0.00648508430609598
GO:BP GO:1904062 regulation of cation transmembrane transport 0.025293744745553 360 41 5 0.121951219512195 0.0138888888888889
GO:BP GO:0042110 T cell activation 0.0269680740024054 489 19 4 0.210526315789474 0.0081799591002045
GO:BP GO:0051239 regulation of multicellular organismal process 0.0269680740024054 2861 55 18 0.327272727272727 0.00629150646627053
GO:BP GO:0001816 cytokine production 0.0269680740024054 844 19 5 0.263157894736842 0.00592417061611374
GO:BP GO:0050870 positive regulation of T cell activation 0.0269680740024054 219 19 3 0.157894736842105 0.0136986301369863
GO:BP GO:0080134 regulation of response to stress 0.027159225046311 1443 29 8 0.275862068965517 0.00554400554400554
GO:BP GO:0045596 negative regulation of cell differentiation 0.0279695765372423 697 14 4 0.285714285714286 0.00573888091822095
GO:BP GO:0001933 negative regulation of protein phosphorylation 0.0279695765372423 373 25 4 0.16 0.0107238605898123
GO:BP GO:0045089 positive regulation of innate immune response 0.0280945069132146 216 20 3 0.15 0.0138888888888889
GO:BP GO:0050793 regulation of developmental process 0.0286221539107057 2605 14 7 0.5 0.00268714011516315
GO:BP GO:0050865 regulation of cell activation 0.029396624068794 655 25 5 0.2 0.00763358778625954
GO:BP GO:0002028 regulation of sodium ion transport 0.029396624068794 87 50 3 0.06 0.0344827586206897
GO:BP GO:0034765 regulation of ion transmembrane transport 0.0305367855349465 583 41 6 0.146341463414634 0.0102915951972556
GO:BP GO:0032101 regulation of response to external stimulus 0.0308292339011256 1051 50 9 0.18 0.00856327307326356
GO:BP GO:0035023 regulation of Rho protein signal transduction 0.0308292339011256 86 54 3 0.0555555555555556 0.0348837209302326
GO:BP GO:0051480 regulation of cytosolic calcium ion concentration 0.0308292339011256 360 67 6 0.0895522388059701 0.0166666666666667
GO:BP GO:1903039 positive regulation of leukocyte cell-cell adhesion 0.0308292339011256 241 19 3 0.157894736842105 0.012448132780083
GO:BP GO:0034762 regulation of transmembrane transport 0.0308292339011256 588 41 6 0.146341463414634 0.0102040816326531
GO:BP GO:0006955 immune response 0.0308292339011256 2311 20 8 0.4 0.00346170488965816
GO:BP GO:0051249 regulation of lymphocyte activation 0.0308292339011256 518 20 4 0.2 0.00772200772200772
GO:BP GO:0002443 leukocyte mediated immunity 0.0308292339011256 877 20 5 0.25 0.00570125427594071
GO:BP GO:0002252 immune effector process 0.030846792772955 1309 20 6 0.3 0.00458365164247517
GO:BP GO:0002833 positive regulation of response to biotic stimulus 0.03144377764928 250 20 3 0.15 0.012
GO:BP GO:0045321 leukocyte activation 0.03144377764928 1332 20 6 0.3 0.0045045045045045
GO:BP GO:0042326 negative regulation of phosphorylation 0.03144377764928 420 25 4 0.16 0.00952380952380952
GO:BP GO:0045638 negative regulation of myeloid cell differentiation 0.0343652510767683 94 54 3 0.0555555555555556 0.0319148936170213
GO:BP GO:0046578 regulation of Ras protein signal transduction 0.035860910931695 199 54 4 0.0740740740740741 0.0201005025125628
GO:BP GO:0022409 positive regulation of cell-cell adhesion 0.036055242951049 284 19 3 0.157894736842105 0.0105633802816901
GO:BP GO:0032412 regulation of ion transmembrane transporter activity 0.036055242951049 270 41 4 0.0975609756097561 0.0148148148148148
GO:BP GO:0090092 regulation of transmembrane receptor protein serine/threonine kinase signaling pathway 0.036055242951049 257 21 3 0.142857142857143 0.0116731517509728
GO:BP GO:0022407 regulation of cell-cell adhesion 0.036055242951049 445 25 4 0.16 0.00898876404494382
GO:BP GO:0006641 triglyceride metabolic process 0.036055242951049 113 47 3 0.0638297872340425 0.0265486725663717
GO:BP GO:0007155 cell adhesion 0.0365540672413568 1492 25 7 0.28 0.0046916890080429
GO:BP GO:0001775 cell activation 0.0367635187521288 1495 25 7 0.28 0.00468227424749164
GO:BP GO:0022610 biological adhesion 0.0369076244727275 1499 25 7 0.28 0.00466977985323549
GO:BP GO:0007200 phospholipase C-activating G protein-coupled receptor signaling pathway 0.0376723490663571 102 54 3 0.0555555555555556 0.0294117647058824
GO:BP GO:0007165 signal transduction 0.0376723490663571 6309 61 32 0.524590163934426 0.00507211919480108
GO:BP GO:0051050 positive regulation of transport 0.0378945063847279 923 30 6 0.2 0.00650054171180932
GO:BP GO:0022898 regulation of transmembrane transporter activity 0.0386057601135662 281 41 4 0.0975609756097561 0.0142348754448399
GO:BP GO:0050776 regulation of immune response 0.0403189290421689 996 20 5 0.25 0.00502008032128514
GO:BP GO:0045087 innate immune response 0.0404567464977738 998 20 5 0.25 0.00501002004008016
GO:BP GO:0051716 cellular response to stimulus 0.0404812621970238 7781 61 37 0.60655737704918 0.00475517285695926
GO:BP GO:0002694 regulation of leukocyte activation 0.0406396024047909 610 20 4 0.2 0.00655737704918033
GO:BP GO:0032409 regulation of transporter activity 0.0429113312217531 297 41 4 0.0975609756097561 0.0134680134680135
GO:BP GO:0048583 regulation of response to stimulus 0.0454626293913635 4278 30 14 0.466666666666667 0.00327255726975222
GO:BP GO:0006469 negative regulation of protein kinase activity 0.0459172925176955 251 25 3 0.12 0.0119521912350598
GO:BP GO:0009968 negative regulation of signal transduction 0.0459172925176955 1364 29 7 0.241379310344828 0.00513196480938416
GO:BP GO:1903037 regulation of leukocyte cell-cell adhesion 0.0459295076140394 338 19 3 0.157894736842105 0.00887573964497041
GO:BP GO:0035335 peptidyl-tyrosine dephosphorylation 0.0459295076140394 104 61 3 0.0491803278688525 0.0288461538461538
GO:BP GO:0006950 response to stress 0.0459295076140394 4170 34 15 0.441176470588235 0.00359712230215827
GO:BP GO:0002684 positive regulation of immune system process 0.0459295076140394 1065 20 5 0.25 0.00469483568075117
GO:BP GO:0071417 cellular response to organonitrogen compound 0.0459295076140394 666 73 8 0.10958904109589 0.012012012012012
GO:BP GO:0050863 regulation of T cell activation 0.0459295076140394 337 19 3 0.157894736842105 0.00890207715133531
GO:BP GO:0090287 regulation of cellular response to growth factor stimulus 0.0474090885514437 315 21 3 0.142857142857143 0.00952380952380952
GO:BP GO:0001824 blastocyst development 0.0487304473047617 104 63 3 0.0476190476190476 0.0288461538461538
GO:BP GO:1901216 positive regulation of neuron death 0.0488493606780079 98 67 3 0.0447761194029851 0.0306122448979592
GO:BP GO:0060326 cell chemotaxis 0.0488610637191969 310 67 5 0.0746268656716418 0.0161290322580645
GO:BP GO:0006639 acylglycerol metabolic process 0.0488610637191969 141 47 3 0.0638297872340425 0.0212765957446809
GO:BP GO:1903532 positive regulation of secretion by cell 0.0488610637191969 285 73 5 0.0684931506849315 0.0175438596491228
GO:BP GO:0002761 regulation of myeloid leukocyte differentiation 0.0489832793047248 123 54 3 0.0555555555555556 0.024390243902439
GO:BP GO:0006638 neutral lipid metabolic process 0.0492339629083194 142 47 3 0.0638297872340425 0.0211267605633803
GO:CC GO:0071944 cell periphery 0.00310090429459964 6169 70 40 0.571428571428571 0.00648403306856865
GO:CC GO:0031226 intrinsic component of plasma membrane 0.00310090429459964 1726 56 16 0.285714285714286 0.00926998841251448
GO:CC GO:0005887 integral component of plasma membrane 0.00480961663271583 1645 56 15 0.267857142857143 0.00911854103343465
GO:CC GO:0031224 intrinsic component of membrane 0.00540353711455453 5868 13 11 0.846153846153846 0.00187457396046353
GO:CC GO:0005886 plasma membrane 0.00540353711455453 5681 67 35 0.522388059701492 0.00616088716775216
GO:CC GO:0016021 integral component of membrane 0.00740252405139304 5711 12 10 0.833333333333333 0.00175100682892663
GO:MF GO:0032394 MHC class Ib receptor activity 3.68118895364636e-06 5 20 3 0.15 0.6
GO:MF GO:0001614 purinergic nucleotide receptor activity 3.75398360862e-05 22 50 4 0.08 0.181818181818182
GO:MF GO:0016502 nucleotide receptor activity 3.75398360862e-05 22 50 4 0.08 0.181818181818182
GO:MF GO:0045028 G protein-coupled purinergic nucleotide receptor activity 0.000163115186263715 15 31 3 0.0967741935483871 0.2
GO:MF GO:0038023 signaling receptor activity 0.00109103908934362 1458 57 15 0.263157894736842 0.0102880658436214
GO:MF GO:0060089 molecular transducer activity 0.00109103908934362 1458 57 15 0.263157894736842 0.0102880658436214
GO:MF GO:0004888 transmembrane signaling receptor activity 0.00354259675808178 1269 57 13 0.228070175438596 0.0102442868400315
GO:MF GO:0140375 immune receptor activity 0.00354259675808178 133 33 4 0.121212121212121 0.0300751879699248
GO:MF GO:0019899 enzyme binding 0.0197833414120017 2074 30 10 0.333333333333333 0.00482160077145612
GO:MF GO:0004930 G protein-coupled receptor activity 0.0338884975882387 839 54 8 0.148148148148148 0.00953516090584029
GO:MF GO:0030246 carbohydrate binding 0.0338884975882387 275 69 5 0.072463768115942 0.0181818181818182
GO:MF GO:0004725 protein tyrosine phosphatase activity 0.0370943802306698 103 61 3 0.0491803278688525 0.029126213592233
GO:MF GO:0031625 ubiquitin protein ligase binding 0.0446847970513664 296 25 3 0.12 0.0101351351351351
GO:MF GO:0044389 ubiquitin-like protein ligase binding 0.0489681205204491 314 25 3 0.12 0.00955414012738853

pTNI14 transcription factor target enrichment of top program genes

TF enrichment of pTNI14
TFs corrosponding to activation of pTNI14. (Left) pearson correlation of TF expression and gene expression program activatity in T/NK/ILC cells, (right) negative log p-value for enrichment of TF binding sites among top uniqueness weighted genes for the gene program. Blue bars indicate significant TFs meeting the following criteria: FDR<0.1, overlap > 2, Corr. R > 0, permutation based p-value < 0.05.